PMID- 25911599
OWN - NLM
STAT- MEDLINE
DCOM- 20160411
LR  - 20150606
IS  - 1472-6491 (Electronic)
IS  - 1472-6483 (Linking)
VI  - 30
IP  - 6
DP  - 2015 Jun
TI  - Altered amphiregulin expression induced by diverse luteinizing hormone receptor 
      reactivity in granulosa cells affects IVF outcomes.
PG  - 593-601
LID - S1472-6483(15)00109-1 [pii]
LID - 10.1016/j.rbmo.2015.03.001 [doi]
AB  - The expression of specific genes (LHR, AREG, EREG, EGFR, NPPC and NPR2) involved 
      in peri-ovulatory signalling pathways induced by LH surge in granulosa cells was 
      investigated, and their relationships with IVF outcomes analysed. mRNA levels of 
      the genes of 147 infertile women undergoing IVF and intracytoplasmic sperm 
      injection (ICSI) with embryo transfer were evaluated. Compared with non-pregnant 
      women, amphiregulin (AREG) mRNA levels in mural and cumulus graunulosa cells were 
      significantly higher (P < 0.05) in pregnant women, and were positively correlated 
      with number of oocytes retrieved and good-quality embryos. No significant 
      differences were found between the two groups in the remaining detected genes. To 
      investigate the reason for the differences in AREG expression, mural granulosa 
      cells were cultured and stimulated with human chorionic gonadotrophin (HCG) for 
      2-24 h. At 4 h after HCG stimulation, AREG and epiregulin mRNA expression peaked, 
      with much greater increases in the pregnant group. The fold-change of AREG 
      expression was positively correlated with number of good-quality embryos. No 
      obvious correlation, however, was found between NPPC/Npr2 expression levels in 
      granulosa cells and IVF outcomes. Altered AREG expression induced by diverse 
      luteinizing hormone receptor reactivity in granulosa cells may provide a useful 
      marker for oocyte developmental competency.
CI  - Copyright (c) 2015. Published by Elsevier Ltd.
FAU - Huang, Ying
AU  - Huang Y
AD  - Reproductive Medical Center, Department of Obstetrics and Gynecology, Peking 
      University Third Hospital, Beijing, China; Key Laboratory of Assisted 
      Reproduction, Ministry of Education, Beijing, China; Beijing Key Laboratory of 
      Reproductive Endocrinology and Assisted Reproductive Technology, Beijing, China.
FAU - Zhao, Yue
AU  - Zhao Y
AD  - Reproductive Medical Center, Department of Obstetrics and Gynecology, Peking 
      University Third Hospital, Beijing, China; Key Laboratory of Assisted 
      Reproduction, Ministry of Education, Beijing, China.
FAU - Yu, Yang
AU  - Yu Y
AD  - Reproductive Medical Center, Department of Obstetrics and Gynecology, Peking 
      University Third Hospital, Beijing, China; Key Laboratory of Assisted 
      Reproduction, Ministry of Education, Beijing, China.
FAU - Li, Rong
AU  - Li R
AD  - Reproductive Medical Center, Department of Obstetrics and Gynecology, Peking 
      University Third Hospital, Beijing, China; Beijing Key Laboratory of Reproductive 
      Endocrinology and Assisted Reproductive Technology, Beijing, China.
FAU - Lin, Shengli
AU  - Lin S
AD  - Reproductive Medical Center, Department of Obstetrics and Gynecology, Peking 
      University Third Hospital, Beijing, China; Beijing Key Laboratory of Reproductive 
      Endocrinology and Assisted Reproductive Technology, Beijing, China.
FAU - Zhang, Chunmei
AU  - Zhang C
AD  - Reproductive Medical Center, Department of Obstetrics and Gynecology, Peking 
      University Third Hospital, Beijing, China; Key Laboratory of Assisted 
      Reproduction, Ministry of Education, Beijing, China.
FAU - Liu, Ping
AU  - Liu P
AD  - Reproductive Medical Center, Department of Obstetrics and Gynecology, Peking 
      University Third Hospital, Beijing, China; Beijing Key Laboratory of Reproductive 
      Endocrinology and Assisted Reproductive Technology, Beijing, China.
FAU - Qiao, Jie
AU  - Qiao J
AD  - Reproductive Medical Center, Department of Obstetrics and Gynecology, Peking 
      University Third Hospital, Beijing, China; Key Laboratory of Assisted 
      Reproduction, Ministry of Education, Beijing, China; Beijing Key Laboratory of 
      Reproductive Endocrinology and Assisted Reproductive Technology, Beijing, China. 
      Electronic address: jie.qiao@263.net.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150314
PL  - Netherlands
TA  - Reprod Biomed Online
JT  - Reproductive biomedicine online
JID - 101122473
RN  - 0 (Amphiregulin)
RN  - 0 (Receptors, LH)
SB  - IM
MH  - Adult
MH  - Amphiregulin/*metabolism
MH  - Case-Control Studies
MH  - Female
MH  - *Fertilization in Vitro
MH  - Granulosa Cells/*physiology
MH  - Humans
MH  - Pregnancy
MH  - Receptors, LH/*physiology
OTO - NOTNLM
OT  - IVF outcome
OT  - LH receptor
OT  - LH surge
OT  - amphiregulin
OT  - granulosa cell
EDAT- 2015/04/26 06:00
MHDA- 2016/04/12 06:00
CRDT- 2015/04/26 06:00
PHST- 2014/09/15 00:00 [received]
PHST- 2015/03/02 00:00 [revised]
PHST- 2015/03/03 00:00 [accepted]
PHST- 2015/04/26 06:00 [entrez]
PHST- 2015/04/26 06:00 [pubmed]
PHST- 2016/04/12 06:00 [medline]
AID - S1472-6483(15)00109-1 [pii]
AID - 10.1016/j.rbmo.2015.03.001 [doi]
PST - ppublish
SO  - Reprod Biomed Online. 2015 Jun;30(6):593-601. doi: 10.1016/j.rbmo.2015.03.001. 
      Epub 2015 Mar 14.