PMID- 25912449
OWN - NLM
STAT- MEDLINE
DCOM- 20160610
LR  - 20181202
IS  - 1347-4421 (Electronic)
IS  - 1347-4421 (Linking)
VI  - 120
IP  - 5
DP  - 2015 Nov
TI  - Novel glucose dehydrogenase from Mucor prainii: Purification, characterization, 
      molecular cloning and gene expression in Aspergillus sojae.
PG  - 498-503
LID - S1389-1723(15)00138-3 [pii]
LID - 10.1016/j.jbiosc.2015.03.012 [doi]
AB  - Glucose dehydrogenase (GDH) is of interest for its potential applications in the 
      field of glucose sensors. To improve the performance of glucose sensors, GDH is 
      required to have strict substrate specificity. A novel flavin adenine 
      dinucleotide (FAD)-dependent GDH was isolated from Mucor prainii NISL0103 and its 
      enzymatic properties were characterized. This FAD-dependent GDH (MpGDH) exhibited 
      high specificity toward glucose. High specificity for glucose was also observed 
      even in the presence of saccharides such as maltose, galactose and xylose. The 
      molecular masses of the glycoforms of GDH ranged from 90 to 130 kDa. After 
      deglycosylation, a single 80 kDa band was observed. The gene encoding MpGDH was 
      cloned and expressed in Aspergillus sojae. The apparent kcat and Km values of 
      recombinant enzyme for glucose were found to be 749.7 s(-1) and 28.3 mM, 
      respectively. The results indicated that the characteristics of MpGDH were 
      suitable for assaying blood glucose levels.
CI  - Copyright (c) 2015 The Society for Biotechnology, Japan. Published by Elsevier B.V. 
      All rights reserved.
FAU - Satake, Ryoko
AU  - Satake R
AD  - Research and Development Division, Kikkoman Corporation, 399 Noda, Chiba 
      278-0037, Japan. Electronic address: rsatake@mail.kikkoman.co.jp.
FAU - Ichiyanagi, Atsushi
AU  - Ichiyanagi A
AD  - Research and Development Division, Kikkoman Corporation, 399 Noda, Chiba 
      278-0037, Japan.
FAU - Ichikawa, Keiichi
AU  - Ichikawa K
AD  - Research and Development Division, Kikkoman Corporation, 399 Noda, Chiba 
      278-0037, Japan.
FAU - Hirokawa, Kozo
AU  - Hirokawa K
AD  - Research and Development Division, Kikkoman Corporation, 399 Noda, Chiba 
      278-0037, Japan.
FAU - Araki, Yasuko
AU  - Araki Y
AD  - Research and Development Division, Kikkoman Corporation, 399 Noda, Chiba 
      278-0037, Japan.
FAU - Yoshimura, Taro
AU  - Yoshimura T
AD  - Research and Development Division, Kikkoman Corporation, 399 Noda, Chiba 
      278-0037, Japan.
FAU - Gomi, Keiko
AU  - Gomi K
AD  - Research and Development Division, Kikkoman Corporation, 399 Noda, Chiba 
      278-0037, Japan.
LA  - eng
PT  - Journal Article
DEP - 20150423
PL  - Japan
TA  - J Biosci Bioeng
JT  - Journal of bioscience and bioengineering
JID - 100888800
RN  - 146-14-5 (Flavin-Adenine Dinucleotide)
RN  - 69-79-4 (Maltose)
RN  - A1TA934AKO (Xylose)
RN  - EC 1.1.1.47 (Glucose 1-Dehydrogenase)
RN  - IY9XDZ35W2 (Glucose)
RN  - X2RN3Q8DNE (Galactose)
SB  - IM
MH  - Aspergillus/*genetics
MH  - Cloning, Molecular
MH  - Flavin-Adenine Dinucleotide/metabolism
MH  - Galactose/metabolism/pharmacology
MH  - Gene Expression
MH  - Glucose/*metabolism/pharmacology
MH  - Glucose 1-Dehydrogenase/chemistry/genetics/*isolation & purification/*metabolism
MH  - Glycosylation
MH  - Maltose/metabolism/pharmacology
MH  - Molecular Weight
MH  - Mucor/*enzymology
MH  - Substrate Specificity/drug effects
MH  - Xylose/metabolism/pharmacology
OTO - NOTNLM
OT  - Diabetes
OT  - Flavin adenine dinucleotide
OT  - Glucose dehydrogenase
OT  - Glucose sensor
OT  - Substrate specificity
EDAT- 2015/04/29 06:00
MHDA- 2016/06/11 06:00
CRDT- 2015/04/28 06:00
PHST- 2014/12/19 00:00 [received]
PHST- 2015/03/11 00:00 [revised]
PHST- 2015/03/16 00:00 [accepted]
PHST- 2015/04/28 06:00 [entrez]
PHST- 2015/04/29 06:00 [pubmed]
PHST- 2016/06/11 06:00 [medline]
AID - S1389-1723(15)00138-3 [pii]
AID - 10.1016/j.jbiosc.2015.03.012 [doi]
PST - ppublish
SO  - J Biosci Bioeng. 2015 Nov;120(5):498-503. doi: 10.1016/j.jbiosc.2015.03.012. Epub 
      2015 Apr 23.