PMID- 25916916
OWN - NLM
STAT- MEDLINE
DCOM- 20160301
LR  - 20180409
IS  - 1873-6351 (Electronic)
IS  - 0278-6915 (Linking)
VI  - 81
DP  - 2015 Jul
TI  - Immunomodulatory activity of methanolic extract of Amorphophallus commutatus var. 
      wayanadensis under normal and cyclophosphamide induced immunosuppressive 
      conditions in mice models.
PG  - 151-159
LID - S0278-6915(15)00143-X [pii]
LID - 10.1016/j.fct.2015.04.026 [doi]
AB  - The present study investigates the immunomodulatory activity of methanolic 
      extract of Amorphophallus commutatus var. wayanadensis (MEAC) under normal and 
      cyclophosphamide induced immunosuppressive conditions in Swiss albino mice 
      models. The splenocyte proliferation assay was performed to study in-vitro 
      immunomodulatory activity of MEAC, where sheep RBC (SRBC) was used to induce 
      immune responses in the experimental animals. The in-vivo immunomodulatory 
      activity was evaluated by humoral antibody titer, quantification of plaque 
      forming cells, qualitative hemolysis, delayed type hypersensitivity assay, 
      phagocytic index and neutrophil adhesion assays. The chemoprotective effect of 
      MEAC was determined against cyclophosphamide induced immunosuppression in mice 
      models. MEAC exhibited significant mitogenic and co-mitogenic activity on Con-A, 
      PHA and LPS stimulated splenocytes isolated from mouse spleen in a dose dependent 
      manner. Furthermore, MEAC also elicited significant immunomodulatory activity 
      with enhanced activation of humoral immune response along with a suppressive 
      effect on cell mediated immune response. Hematological and histopathological 
      analysis revealed the protective effect of MEAC against CP induced 
      immunosuppression. The significant immunomodulatory activity of MEAC observed in 
      the current study could be due to the fatty acids and phytosterols present in the 
      extract.
CI  - Copyright (c) 2015 Elsevier Ltd. All rights reserved.
FAU - Raj, Sreena
AU  - Raj S
AD  - School of Bio Sciences and Technology, VIT University, Vellore 632014, India.
FAU - Gothandam, K M
AU  - Gothandam KM
AD  - School of Bio Sciences and Technology, VIT University, Vellore 632014, India. 
      Electronic address: gothandam@yahoo.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150424
PL  - England
TA  - Food Chem Toxicol
JT  - Food and chemical toxicology : an international journal published for the British 
      Industrial Biological Research Association
JID - 8207483
RN  - 0 (Fatty Acids)
RN  - 0 (Immunosuppressive Agents)
RN  - 0 (Phytochemicals)
RN  - 0 (Phytosterols)
RN  - 0 (Plant Extracts)
RN  - 8N3DW7272P (Cyclophosphamide)
RN  - Y4S76JWI15 (Methanol)
SB  - IM
MH  - Amorphophallus/*chemistry
MH  - Animals
MH  - Cell Adhesion/drug effects
MH  - Cell Proliferation/drug effects
MH  - Cyclophosphamide/*toxicity
MH  - Dose-Response Relationship, Drug
MH  - Fatty Acids/analysis/pharmacology
MH  - Gas Chromatography-Mass Spectrometry
MH  - Hypersensitivity, Delayed/chemically induced/drug therapy
MH  - Immunity, Humoral/drug effects
MH  - *Immunomodulation
MH  - Immunosuppressive Agents/*toxicity
MH  - Methanol
MH  - Mice
MH  - Neutrophils/cytology/drug effects
MH  - Phytochemicals/pharmacology
MH  - Phytosterols/analysis/pharmacology
MH  - Plant Extracts/*pharmacology
MH  - Spleen/cytology/drug effects
OTO - NOTNLM
OT  - Amorphophallus commutatus var. wayanadensis
OT  - Fatty acids
OT  - GC-MS
OT  - Immunomodulatory activity
OT  - Immunosuppression
OT  - Phytosterols
EDAT- 2015/04/29 06:00
MHDA- 2016/03/02 06:00
CRDT- 2015/04/29 06:00
PHST- 2014/12/19 00:00 [received]
PHST- 2015/03/26 00:00 [revised]
PHST- 2015/04/20 00:00 [accepted]
PHST- 2015/04/29 06:00 [entrez]
PHST- 2015/04/29 06:00 [pubmed]
PHST- 2016/03/02 06:00 [medline]
AID - S0278-6915(15)00143-X [pii]
AID - 10.1016/j.fct.2015.04.026 [doi]
PST - ppublish
SO  - Food Chem Toxicol. 2015 Jul;81:151-159. doi: 10.1016/j.fct.2015.04.026. Epub 2015 
      Apr 24.