PMID- 25916988
OWN - NLM
STAT- MEDLINE
DCOM- 20150825
LR  - 20181113
IS  - 1098-5522 (Electronic)
IS  - 0019-9567 (Print)
IS  - 0019-9567 (Linking)
VI  - 83
IP  - 7
DP  - 2015 Jul
TI  - Dynamic Virus-Bacterium Interactions in a Porcine Precision-Cut Lung Slice 
      Coinfection Model: Swine Influenza Virus Paves the Way for Streptococcus suis 
      Infection in a Two-Step Process.
PG  - 2806-15
LID - 10.1128/IAI.00171-15 [doi]
AB  - Swine influenza virus (SIV) and Streptococcus suis are common pathogens of the 
      respiratory tract in pigs, with both being associated with pneumonia. The 
      interactions of both pathogens and their contribution to copathogenesis are only 
      poorly understood. In the present study, we established a porcine precision-cut 
      lung slice (PCLS) coinfection model and analyzed the effects of a primary SIV 
      infection on secondary infection by S. suis at different time points. We found 
      that SIV promoted adherence, colonization, and invasion of S. suis in a two-step 
      process. First, in the initial stages, these effects were dependent on bacterial 
      encapsulation, as shown by selective adherence of encapsulated, but not 
      unencapsulated, S. suis to SIV-infected cells. Second, at a later stage of 
      infection, SIV promoted S. suis adherence and invasion of deeper tissues by 
      damaging ciliated epithelial cells. This effect was seen with a highly virulent 
      SIV subtype H3N2 strain but not with a low-virulence subtype H1N1 strain, and it 
      was independent of the bacterial capsule, since an unencapsulated S. suis mutant 
      behaved in a way similar to that of the encapsulated wild-type strain. In 
      conclusion, the PCLS coinfection model established here revealed novel insights 
      into the dynamic interactions between SIV and S. suis during infection of the 
      respiratory tract. It showed that at least two different mechanisms contribute to 
      the beneficial effects of SIV for S. suis, including capsule-mediated bacterial 
      attachment to SIV-infected cells and capsule-independent effects involving 
      virus-mediated damage of ciliated epithelial cells.
CI  - Copyright (c) 2015, American Society for Microbiology. All Rights Reserved.
FAU - Meng, F
AU  - Meng F
AD  - Institute for Virology, University of Veterinary Medicine Hannover, Hannover, 
      Germany.
FAU - Wu, N H
AU  - Wu NH
AD  - Institute for Virology, University of Veterinary Medicine Hannover, Hannover, 
      Germany.
FAU - Nerlich, A
AU  - Nerlich A
AD  - Institute for Microbiology, University of Veterinary Medicine Hannover, Hannover, 
      Germany.
FAU - Herrler, G
AU  - Herrler G
AD  - Institute for Virology, University of Veterinary Medicine Hannover, Hannover, 
      Germany.
FAU - Valentin-Weigand, P
AU  - Valentin-Weigand P
AD  - Institute for Microbiology, University of Veterinary Medicine Hannover, Hannover, 
      Germany P.valentin@tiho-hannover.de.
FAU - Seitz, M
AU  - Seitz M
AD  - Institute for Microbiology, University of Veterinary Medicine Hannover, Hannover, 
      Germany.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150427
PL  - United States
TA  - Infect Immun
JT  - Infection and immunity
JID - 0246127
SB  - IM
MH  - Animals
MH  - *Coinfection
MH  - Disease Models, Animal
MH  - Epithelial Cells/pathology
MH  - Influenza A Virus, H3N2 Subtype/growth & development
MH  - Lung/microbiology/*pathology/virology
MH  - Models, Theoretical
MH  - Orthomyxoviridae Infections/complications/*pathology
MH  - Pneumonia, Bacterial/complications/*pathology
MH  - Pneumonia, Viral/complications/*pathology
MH  - Streptococcal Infections/complications/*pathology
MH  - Streptococcus suis/growth & development
MH  - Swine
MH  - Time Factors
PMC - PMC4468551
EDAT- 2015/04/29 06:00
MHDA- 2015/08/26 06:00
PMCR- 2016/01/01
CRDT- 2015/04/29 06:00
PHST- 2015/02/11 00:00 [received]
PHST- 2015/04/21 00:00 [accepted]
PHST- 2015/04/29 06:00 [entrez]
PHST- 2015/04/29 06:00 [pubmed]
PHST- 2015/08/26 06:00 [medline]
PHST- 2016/01/01 00:00 [pmc-release]
AID - IAI.00171-15 [pii]
AID - 00171-15 [pii]
AID - 10.1128/IAI.00171-15 [doi]
PST - ppublish
SO  - Infect Immun. 2015 Jul;83(7):2806-15. doi: 10.1128/IAI.00171-15. Epub 2015 Apr 
      27.