PMID- 25917455
OWN - NLM
STAT- MEDLINE
DCOM- 20160323
LR  - 20161125
IS  - 1872-8057 (Electronic)
IS  - 0303-7207 (Linking)
VI  - 411
DP  - 2015 Aug 15
TI  - Molecular characterization of a disintegrin and metalloprotease-17 (ADAM17) in 
      granulosa cells of bovine preovulatory follicles.
PG  - 49-57
LID - S0303-7207(15)00196-3 [pii]
LID - 10.1016/j.mce.2015.04.010 [doi]
AB  - A disintegrin and metalloprotease-17 (ADAM17) is thought to play a key role in 
      the release of soluble and active epiregulin (EREG) and amphiregulin (AREG) in 
      ovarian follicles but its transcriptional regulation in follicular cells remains 
      largely unknown. The objectives of this study were to characterize the regulation 
      of ADAM17 transcripts in bovine follicles prior to ovulation and to investigate 
      its transcriptional control in bovine granulosa cells. To study the regulation of 
      ADAM17 transcripts, RT-PCR analyses were performed using total RNA extracted from 
      bovine follicles collected between 0 h and 24 h post-hCG. Results showed that 
      levels of ADAM17 mRNA were low prior to hCG (0 h), markedly and transiently 
      increased 6-12 h post-hCG (P <0.05), and returned to low baseline levels at 24 h 
      post-hCG in granulosa and theca interna cells of preovulatory follicles. To 
      determine the transcriptional control of ADAM17 expression, primary cultures of 
      bovine granulosa cells were used. Forskolin (FSK) stimulation induced a pattern 
      of ADAM17 mRNA up-regulation in vitro similar to that observed by hCG in vivo. 
      5'-Deletion mutagenesis studies identified a minimal region of the bovine ADAM17 
      promoter containing basal and FSK-inducible activities, which were dependent on 
      the presence of a consensus AP1 cis-element. Electrophoretic mobility shift 
      assays revealed an interaction between AP1 and the trans-acting factor Fra2. 
      Chromatin immunoprecipitation assays confirmed an endogenous interaction between 
      Fra2 and the ADAM17 promoter in granulosa cell cultures. FSK-inducible ADAM17 
      promoter activity and mRNA expression were suppressed by PKA and ERK1/2 
      inhibitors but not by a p38MAPK inhibitor, pointing to the importance of PKA and 
      ERK1/2 signaling pathways in the up-regulation of bovine ADAM17 mRNA. 
      Collectively, these findings describe the gonadotropin/FSK-dependent 
      up-regulation of ADAM17 transcripts in bovine preovulatory follicles and unravel 
      for the first time some of the molecular mechanisms involved in ADAM17 gene 
      expression in granulosa cells of a monoovulatory species.
CI  - Copyright (c) 2015 Elsevier Ireland Ltd. All rights reserved.
FAU - Sayasith, Khampoun
AU  - Sayasith K
AD  - Centre de Recherche en Reproduction Animale, Departement de Biomedecine 
      Veterinaire, Faculte de Medecine Veterinaire, Universite de Montreal, 
      Saint-Hyacinthe, Quebec J2S 7C6, Canada. Electronic address: 
      k.sayasith@umontreal.ca.
FAU - Sirois, Jean
AU  - Sirois J
AD  - Centre de Recherche en Reproduction Animale, Departement de Biomedecine 
      Veterinaire, Faculte de Medecine Veterinaire, Universite de Montreal, 
      Saint-Hyacinthe, Quebec J2S 7C6, Canada.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150424
PL  - Ireland
TA  - Mol Cell Endocrinol
JT  - Molecular and cellular endocrinology
JID - 7500844
RN  - 0 (Chorionic Gonadotropin)
RN  - 1F7A44V6OU (Colforsin)
RN  - EC 3.4.24.- (ADAM Proteins)
RN  - EC 3.4.24.86 (ADAM17 Protein)
SB  - IM
MH  - ADAM Proteins/genetics/*metabolism
MH  - ADAM17 Protein
MH  - Animals
MH  - Cattle
MH  - Chorionic Gonadotropin/pharmacology
MH  - Colforsin/pharmacology
MH  - Female
MH  - Gene Expression Regulation/drug effects
MH  - Granulosa Cells/drug effects/*metabolism
MH  - Ovarian Follicle/drug effects/*metabolism
MH  - Ovulation/genetics
MH  - Promoter Regions, Genetic/drug effects
MH  - Up-Regulation/drug effects
OTO - NOTNLM
OT  - ADAM17 up-regulation
OT  - Gonadotropin
OT  - Ovulatory process
OT  - Preovulatory follicles
OT  - Primary granulosa cells
OT  - Promoter activation
EDAT- 2015/04/29 06:00
MHDA- 2016/03/24 06:00
CRDT- 2015/04/29 06:00
PHST- 2015/03/05 00:00 [received]
PHST- 2015/04/09 00:00 [revised]
PHST- 2015/04/13 00:00 [accepted]
PHST- 2015/04/29 06:00 [entrez]
PHST- 2015/04/29 06:00 [pubmed]
PHST- 2016/03/24 06:00 [medline]
AID - S0303-7207(15)00196-3 [pii]
AID - 10.1016/j.mce.2015.04.010 [doi]
PST - ppublish
SO  - Mol Cell Endocrinol. 2015 Aug 15;411:49-57. doi: 10.1016/j.mce.2015.04.010. Epub 
      2015 Apr 24.