PMID- 25917748 OWN - NLM STAT- MEDLINE DCOM- 20160623 LR - 20150916 IS - 1365-2230 (Electronic) IS - 0307-6938 (Linking) VI - 40 IP - 7 DP - 2015 Oct TI - Differential expression of mRNA for T-bet and GATA-3 transcription factors in peripheral blood mononuclear cells of patients with vitiligo. PG - 735-40 LID - 10.1111/ced.12661 [doi] AB - BACKGROUND: There is accumulating evidence that aberrant T helper (Th)1 and Th2 cell responses play critical roles in the pathogenesis of autoimmune diseases. However, their importance in the pathobiology of vitiligo have yet to be elucidated. AIM: To evaluate the expression profile of two transcription factors, namely, T-bet, a Th1-specific T box transcription factor and GATA binding protein 3 (GATA-3), a Th2-specific transcription factor, and to measure expression levels of interferon (IFN)-gamma and interleukin (IL)-4 mRNAs as the signature cytokines of Th1 and Th2 cells, respectively. METHODS: Gene expression analysis of peripheral blood mononuclear cells (PBMCs) was performed using real-time reverse transcriptase PCR. RESULTS: In patients with vitiligo compared with controls, mRNA expression was significantly higher for T-bet and IFN-gamma, and significantly lower for GATA-3 and IL-4 mRNAs (P