PMID- 25918597
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20150428
LR  - 20201001
IS  - 2008-076X (Print)
IS  - 2008-0778 (Electronic)
IS  - 2008-0778 (Linking)
VI  - 9
IP  - 1
DP  - 2015 Apr-Jun
TI  - Effect of phosalone on testicular tissue and in vitro fertilizing potential.
PG  - 93-106
AB  - BACKGROUND: The current study aimed to evaluate the effects of phosalone (PLN) as 
      an organophosphate (OP) compound on testicular tissue, hormonal alterations and 
      embryo development in rats. MATERIALS AND METHODS: In this experimental study, we 
      divided 18 mature Wistar rats into three groups-control, control-sham and test 
      (n=6 per group). Animals in the test group received one-fourth the lethal dose 
      (LD50) of PLN (150 mg/kg), orally, once per day for 45 days. DNA laddering and 
      epi-fluorescent analyses were performed to evaluate testicular DNA fragmentation 
      and RNA damage, respectively. Serum levels of testosterone and inhibin-B (IN-B) 
      were evaluated. Testicular levels of total antioxidant capacity (TAC), total 
      thiol molecules (TTM) and glutathione peroxidase (GSH-px) were analyzed. Finally, 
      we estimated sperm parameters and effect of PLN on embryo development. Two-way 
      ANOVA was used for statistical analyses. RESULTS: There was severe DNA 
      fragmentation and RNA damage in testicular tissue of animals that received PLN. 
      PLN remarkably (p<0.05) decreased testicular TAC, TTM and GSH-px levels. Animals 
      that received PLN exhibited significantly (p<0.05) decreased serum levels of 
      testosterone and IN-B. Reduced sperm count, viability, motility, chromatin 
      condensation and elevated sperm DNA damage were observed in the test group rats. 
      PLN resulted in significant (p<0.05) reduction of in vitro fertilizing (IVF) 
      potential and elevated embryonic degeneration. CONCLUSION: PLN reduced 
      fertilization potential and embryo development were attributed to a cascade of 
      impacts on the testicles and sperm. PLN promoted its impact by elevating DNA and 
      RNA damages via down-regulation of testicular endocrine activity and antioxidant 
      status.
FAU - Amniattalab, Amir
AU  - Amniattalab A
AD  - Department of Pathology, Faculty of Veterinary Medicine, Urmia Branch, Islamic 
      Azad University, Urmia, Iran.
FAU - Razi, Mazdak
AU  - Razi M
AD  - Department of Comparative Histology and Embryology, Faculty of Veterinary 
      Medicine, Urmia University, Urmia, Iran.
LA  - eng
PT  - Journal Article
DEP - 20150421
PL  - Iran
TA  - Int J Fertil Steril
JT  - International journal of fertility & sterility
JID - 101487941
PMC - PMC4410043
OTO - NOTNLM
OT  - DNA Fragmentation
OT  - In Vitro Fertilization
OT  - Phosalone
OT  - Sperm
OT  - Testicular Tissue
EDAT- 2015/04/29 06:00
MHDA- 2015/04/29 06:01
PMCR- 2015/04/01
CRDT- 2015/04/29 06:00
PHST- 2013/10/06 00:00 [received]
PHST- 2014/01/28 00:00 [accepted]
PHST- 2015/04/29 06:00 [entrez]
PHST- 2015/04/29 06:00 [pubmed]
PHST- 2015/04/29 06:01 [medline]
PHST- 2015/04/01 00:00 [pmc-release]
AID - 10.22074/ijfs.2015.4213 [doi]
PST - ppublish
SO  - Int J Fertil Steril. 2015 Apr-Jun;9(1):93-106. doi: 10.22074/ijfs.2015.4213. Epub 
      2015 Apr 21.