PMID- 25920255
OWN - NLM
STAT- MEDLINE
DCOM- 20150616
LR  - 20150429
IS  - 1934-578X (Print)
IS  - 1555-9475 (Linking)
VI  - 10
IP  - 2
DP  - 2015 Feb
TI  - Proanthocyanidin from grape seed extract inhibits airway inflammation and 
      remodeling in a murine model of chronic asthma.
PG  - 257-62
AB  - Asthma is characterized by airway inflammation and airway remodeling. Our 
      previous study revealed that grape seed proanthocyanidin extract (GSPE) could 
      inhibit asthmatic airway inflammation and airway hyper-responsiveness by 
      down-regulation of inducible nitric oxide synthase in a murine model of acute 
      asthma. The present study aimed to evaluate GSPE's effects on airway inflammation 
      and airway remodeling in a chronic asthmatic model. BALB/c mice were sensitized 
      with ovalbumin (OVA) and then were challenged three times a week for 8 weeks. 
      Airway responsiveness was measured at 24 h after the last OVA challenge. HE 
      staining, PAS staining, and Masson staining were used to observe any airway 
      inflammation in the lung tissue, airway mucus secretion, and subepithelial 
      fibrosis, respectively. The cytokines levels in the lavage fluid (BALF) in 
      addition to the total serum immunoglobulin E (IgE) levels were detected by ELISA. 
      Furthermore, lung collagen contents, alpha-smooth muscle actin (alpha-SMA), and 
      transforming growth factor-beta1 (TGF-beta1) expression in the airway were assessed by 
      hydroxyproline assay, immunohistochemistry, and Western blot analysis, 
      respectively. GSPE administration significantly suppressed airway resistance as 
      well as reduced the amount of inflammatory cells, especially the eosinophil 
      count, in BALF. Additionally, the GSPE treatment markedly decreased interleukin 
      (IL)-4, IL-13, and vascular endothelial growth factor (VEGF) levels in BALF in 
      addition to the total serum IgE levels. A histological examination demonstrated 
      that GSPE significantly ameliorated allergen-induced lung eosinophilic 
      inflammation and decreased PAS-positive epithelial cells in the airway. The 
      elevated hydroxyproline contents, lung alpha-SMA contents, and TGF-beta1 protein 
      expression that were observed in the OVA mice were also inhibited by GSPE. In 
      conclusion, GSPE could inhibit airway inflammation and airway remodeling in a 
      murine model of chronic asthma, thus providing a potential treatment for asthma.
FAU - Zhou, Dan-Yang
AU  - Zhou DY
FAU - Fang, Su-Rong
AU  - Fang SR
FAU - Zou, Chun-Fang
AU  - Zou CF
FAU - Zhang, Qian
AU  - Zhang Q
FAU - Gu, Wei
AU  - Gu W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Nat Prod Commun
JT  - Natural product communications
JID - 101477873
RN  - 0 (Actins)
RN  - 0 (Grape Seed Extract)
RN  - 0 (Proanthocyanidins)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 0 (alpha-smooth muscle actin, mouse)
RN  - 18206-61-6 (proanthocyanidin)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 9006-59-1 (Ovalbumin)
RN  - RMB44WO89X (Hydroxyproline)
SB  - IM
MH  - Actins/genetics/metabolism
MH  - Airway Remodeling/*drug effects
MH  - Animals
MH  - Asthma/*complications/drug therapy
MH  - Bronchoalveolar Lavage Fluid/chemistry
MH  - Female
MH  - Grape Seed Extract/*chemistry
MH  - Hydroxyproline/metabolism
MH  - Immunoglobulin E/metabolism
MH  - Inflammation/*drug therapy/etiology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Ovalbumin
MH  - Proanthocyanidins/chemistry/*therapeutic use
MH  - Transforming Growth Factor beta1/genetics/metabolism
EDAT- 2015/04/30 06:00
MHDA- 2015/06/17 06:00
CRDT- 2015/04/30 06:00
PHST- 2015/04/30 06:00 [entrez]
PHST- 2015/04/30 06:00 [pubmed]
PHST- 2015/06/17 06:00 [medline]
PST - ppublish
SO  - Nat Prod Commun. 2015 Feb;10(2):257-62.