PMID- 25920331
OWN - NLM
STAT- MEDLINE
DCOM- 20160419
LR  - 20150714
IS  - 1559-0291 (Electronic)
IS  - 0273-2289 (Linking)
VI  - 176
IP  - 3
DP  - 2015 Jun
TI  - Effects of Surfactants and Microwave-assisted Pretreatment of Orange Peel on 
      Extracellular Enzymes Production by Aspergillus japonicus PJ01.
PG  - 758-71
LID - 10.1007/s12010-015-1609-z [doi]
AB  - The effects of surfactants and microwave pretreatment of orange peel powder (OPP) 
      on the production of pectinase, cellulase, and xylanase by Aspergillus japonicus 
      PJ01 in submerged fermentation were investigated. The results showed that when 
      OPP was pretreated only by microwave (630 W, 9 min, and liquid/solid ratio 5), 
      the activities of exo-pectinase, carboxymethyl cellulase (CMCase), xylanase, and 
      filter paper cellulase (FPase) were increased by 11.8, 20.6, 16.2, and 24.0 %, 
      respectively, and when OPP was pretreated by microwave at the same conditions 
      cited above plus PEG 4000 at the concentration of 3 g/L, the activities of the 
      above four enzymes were enhanced by 40.2, 30.3, 40.4, and 40.0 % after 84-h 
      cultivation, respectively. It is suggested a synergistic effect between microwave 
      and surfactant treatment in enhancing the multiextracellular enzymes production 
      by OPP fermentation of A. japonicus PJ01. Chemical composition and Fourier 
      transform infrared spectroscopy (FTIR) analysis displayed that the microwave 
      pretreatment of OPP led to the decrease of hemicellulose and essential oil 
      contents. Scanning electron microscope (SEM) showed that OPP surface after 
      microwave pretreatment became porous and more susceptible to be invaded by A. 
      japonicus. The results demonstrated that pretreatment of OPP by surfactant PEG 
      4000 and microwave irradiation as environment-friendly way was cost-effective in 
      enhancing the multienzyme production from agricultural waste orange peel.
FAU - Li, Pei-jun
AU  - Li PJ
AD  - School of Minerals Processing and Bioengineering, Central South University, 
      Changsha, 410083, China.
FAU - Xia, Jin-lan
AU  - Xia JL
FAU - Shan, Yang
AU  - Shan Y
FAU - Nie, Zhen-yuan
AU  - Nie ZY
FAU - Wang, Fei-ran
AU  - Wang FR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150429
PL  - United States
TA  - Appl Biochem Biotechnol
JT  - Applied biochemistry and biotechnology
JID - 8208561
RN  - 0 (Plant Extracts)
RN  - 0 (Surface-Active Agents)
RN  - EC 3.2.1.- (Glycoside Hydrolases)
SB  - IM
MH  - Aspergillus/*cytology/drug effects/growth & development/*metabolism
MH  - Citrus sinensis/*chemistry
MH  - Extracellular Space/*drug effects/metabolism
MH  - Fermentation/drug effects
MH  - Glycoside Hydrolases/*biosynthesis
MH  - *Microwaves
MH  - Plant Extracts/pharmacology
MH  - Surface-Active Agents/*pharmacology
EDAT- 2015/04/30 06:00
MHDA- 2016/04/20 06:00
CRDT- 2015/04/30 06:00
PHST- 2014/09/15 00:00 [received]
PHST- 2015/04/06 00:00 [accepted]
PHST- 2015/04/30 06:00 [entrez]
PHST- 2015/04/30 06:00 [pubmed]
PHST- 2016/04/20 06:00 [medline]
AID - 10.1007/s12010-015-1609-z [doi]
PST - ppublish
SO  - Appl Biochem Biotechnol. 2015 Jun;176(3):758-71. doi: 10.1007/s12010-015-1609-z. 
      Epub 2015 Apr 29.