PMID- 25921798
OWN - NLM
STAT- MEDLINE
DCOM- 20151221
LR  - 20191210
IS  - 1520-6882 (Electronic)
IS  - 0003-2700 (Linking)
VI  - 87
IP  - 10
DP  - 2015 May 19
TI  - Conversion of inhibition biosensing to substrate-like biosensing for quinalphos 
      selective detection.
PG  - 5270-7
LID - 10.1021/acs.analchem.5b00376 [doi]
AB  - Since all of the organophosphorus pesticides (OPP) inhibit the cholinesterases 
      with a common mechanism, it is still challenging to detect OPP selectively with 
      inhibition-based biosensors. This study focuses on the conversion of a typical 
      inhibition biosensing to a selective substrate-like biosensing. The interaction 
      of quinalphos with plant-esterase involves not only a decrease in enzyme activity 
      but also a heterolytic bond cleavage of quinalphos. The leaving group eliminated 
      from quinalphos is an ideal biomarker due to its specificity in most OPP. Thus, 
      using 2-hydroxyquinoxaline (HQO), the leaving group of quinalphos, as the 
      biomarker and meso-tetra (4-sulfonatophenyl) porphine (TPPS4) as an optical 
      probe, quinalphos can be selectively detected. The molecular recognition between 
      TPPS4 and HQO leads to a considerable sensitivity of the detection. The spectral 
      responses of TPPS4 show a linear dependence on quinalphos concentration in the 
      presence of plant-esterase within the 0.01-1 mg kg(-1) range. The detection limit 
      is 0.01 mg kg(-1), well below the maximum residue limits (MRLs) defined by 
      European Union (0.05 mg kg(-1)) and China (0.2 mg kg(-1)).
FAU - Yang, Limin
AU  - Yang L
AD  - State Key Laboratory of Heavy Oil Processing and Center for Bioengineering and 
      Biotechnology, China University of Petroleum (East China), Qingdao, Shandong 
      266555, P. R. China.
FAU - Han, Juan
AU  - Han J
AD  - State Key Laboratory of Heavy Oil Processing and Center for Bioengineering and 
      Biotechnology, China University of Petroleum (East China), Qingdao, Shandong 
      266555, P. R. China.
FAU - Liu, Wei
AU  - Liu W
AD  - State Key Laboratory of Heavy Oil Processing and Center for Bioengineering and 
      Biotechnology, China University of Petroleum (East China), Qingdao, Shandong 
      266555, P. R. China.
FAU - Li, Jiqiang
AU  - Li J
AD  - State Key Laboratory of Heavy Oil Processing and Center for Bioengineering and 
      Biotechnology, China University of Petroleum (East China), Qingdao, Shandong 
      266555, P. R. China.
FAU - Jiang, Lei
AU  - Jiang L
AD  - State Key Laboratory of Heavy Oil Processing and Center for Bioengineering and 
      Biotechnology, China University of Petroleum (East China), Qingdao, Shandong 
      266555, P. R. China.
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150507
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
RN  - 0 (Insecticides)
RN  - 0 (Organothiophosphorus Compounds)
RN  - 0 (Porphyrins)
RN  - 0 (Quinoxalines)
RN  - 1196-57-2 (2-hydroxyquinoxaline)
RN  - 26S837727Y (quinalphos)
RN  - 35218-75-8 (tetraphenylporphine sulfonate)
RN  - EC 3.1.- (Esterases)
SB  - IM
MH  - Biosensing Techniques/*methods
MH  - Esterases/metabolism
MH  - Insecticides/*analysis/metabolism
MH  - Limit of Detection
MH  - Organothiophosphorus Compounds/*analysis/metabolism
MH  - Porphyrins/analysis/metabolism
MH  - Quinoxalines/analysis/metabolism
MH  - Triticum/enzymology
EDAT- 2015/04/30 06:00
MHDA- 2015/12/22 06:00
CRDT- 2015/04/30 06:00
PHST- 2015/04/30 06:00 [entrez]
PHST- 2015/04/30 06:00 [pubmed]
PHST- 2015/12/22 06:00 [medline]
AID - 10.1021/acs.analchem.5b00376 [doi]
PST - ppublish
SO  - Anal Chem. 2015 May 19;87(10):5270-7. doi: 10.1021/acs.analchem.5b00376. Epub 
      2015 May 7.