PMID- 25923080 OWN - NLM STAT- MEDLINE DCOM- 20160222 LR - 20181113 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 16 IP - 5 DP - 2015 Apr 27 TI - An Exploratory Pilot Study of Genetic Marker for IgE-Mediated Allergic Diseases with Expressions of FcepsilonR1alpha and Cepsilon. PG - 9504-19 LID - 10.3390/ijms16059504 [doi] AB - The high affinity immunoglobulin E (IgE) receptor-FcepsilonR1 is mainly expressed on the surface of effector cells. Cross-linking of IgE Abs bound to FcepsilonR1 by multi-valent antigens can induce the activation of these cells and the secretion of inflammatory mediators. Since FcepsilonR1 plays a central role in the induction and maintenance of allergic responses, this study aimed to investigate the association of FcepsilonR1 with the allergic phenotype of Cepsilon expression and cytokine and histamine release from peripheral leukocytes. Peripheral leukocytes from 67 allergic and 50 non-allergic subjects were used for genotyping analysis. Peripheral mononuclear cells (PBMCs) were used for Cepsilon expression and ELISpot analysis, while polymorphonuclear cells (PMNs) were used for histamine release. The association between genotype polymorphism of the FcepsilonR1alpha promoter region (rs2427827 and rs2251746) and allergic features of Cepsilon expression and histamine were analyzed, and their effects on leukocytes function were compared with wild type. The genotype polymorphisms of FcepsilonR1alpha promoter region with CT and TT in rs2427827 and TC in rs2251746 were significantly higher in allergic patients than in non-allergic controls. Patients with single nucleotide polymorphism (SNP) of FcepsilonR1alpha promoter region had high levels of total IgE, mite-specific Der p 2 (Group 2 allergen of Dermatophagoides pteronyssinus)-specific IgE and IgE secretion B cells. The mRNA expression of FcepsilonR1alpha was significantly increased after Der p2 stimulation in PBMCs with SNPs of the FcepsilonR1alpha promoter region. Despite the increased Cepsilon mRNA expression in PBMCs and histamine release from PMNs and the up-regulated mRNA expression of interleukin (IL)-6 and IL-8 secretions after Der p2 stimulation, there was no statistically significant difference between SNPs of the FcepsilonR1alpha promoter region and the wild type. SNPs of FcepsilonR1alpha promoter region were associated with IgE expression, IgE producing B cells, and increased Der p2-induced FcepsilonR1alpha mRNA expression. These SNPs may be used as a disease marker for IgE-mediated allergic inflammation caused by Dermatophagoides pteronyssinus. FAU - Liao, En-Chih AU - Liao EC AD - Center for Translational Medicine, Department of Medical Research, Taichung Veterans General Hospital, Taichung 40705, Taiwan. en65@vghtc.gov.tw. AD - Department of Bio-Industry Technology, Da Yeh University, Changhua 51591, Taiwan. en65@vghtc.gov.tw. AD - Department of Medical Technology, Jen Ten College of Medicine, Nursing and Management, Miaoli 35664, Taiwan. en65@vghtc.gov.tw. FAU - Chang, Ching-Yun AU - Chang CY AD - Division of Allergy, Immunology & Rheumatology, Department of Internal Medicine, Taichung Veterans General Hospital, Taichung 40705, Taiwan. yun0922.tw@yahoo.com.tw. FAU - Hsieh, Chia-Wei AU - Hsieh CW AD - Division of Allergy, Immunology & Rheumatology, Department of Internal Medicine, Taichung Veterans General Hospital, Taichung 40705, Taiwan. chiaweih@gmail.com. FAU - Yu, Sheng-Jie AU - Yu SJ AD - College of Life Sciences, National Chung Hsing University, Taichung 40227, Taiwan. jim0929@msn.com. FAU - Yin, Sui-Chu AU - Yin SC AD - Center for Translational Medicine, Department of Medical Research, Taichung Veterans General Hospital, Taichung 40705, Taiwan. scyin@vghtc.gov.tw. FAU - Tsai, Jaw-Ji AU - Tsai JJ AD - Division of Allergy, Immunology & Rheumatology, Department of Internal Medicine, Taichung Veterans General Hospital, Taichung 40705, Taiwan. jawji@vghtc.gov.tw. AD - College of Life Sciences, National Chung Hsing University, Taichung 40227, Taiwan. jawji@vghtc.gov.tw. AD - Institute of Clinical Medicine, School of Medicine, National Yang Ming University, Taipei 11221, Taiwan. jawji@vghtc.gov.tw. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20150427 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (Antigens, Dermatophagoides) RN - 0 (Arthropod Proteins) RN - 0 (Dermatophagoides pteronyssinus antigen p 2) RN - 0 (FcepsilonRI alpha-chain, human) RN - 0 (Immunoglobulin Constant Regions) RN - 0 (RNA, Messenger) RN - 0 (Receptors, IgE) RN - 37341-29-0 (Immunoglobulin E) SB - IM MH - Adolescent MH - Adult MH - Alleles MH - Animals MH - Antigens, Dermatophagoides/immunology MH - Arthropod Proteins/immunology MH - Child MH - Female MH - Genotype MH - Humans MH - Hypersensitivity, Immediate/*genetics/immunology MH - Immunoglobulin Constant Regions/genetics/*metabolism MH - Immunoglobulin E/biosynthesis MH - Male MH - Middle Aged MH - Neutrophils/cytology MH - Phenotype MH - Pilot Projects MH - Polymorphism, Genetic MH - Polymorphism, Single Nucleotide MH - Promoter Regions, Genetic MH - Pyroglyphidae/immunology MH - RNA, Messenger/metabolism MH - Receptors, IgE/*genetics MH - Young Adult PMC - PMC4463601 EDAT- 2015/04/30 06:00 MHDA- 2016/02/24 06:00 PMCR- 2015/05/01 CRDT- 2015/04/30 06:00 PHST- 2014/12/11 00:00 [received] PHST- 2015/04/10 00:00 [revised] PHST- 2015/04/20 00:00 [accepted] PHST- 2015/04/30 06:00 [entrez] PHST- 2015/04/30 06:00 [pubmed] PHST- 2016/02/24 06:00 [medline] PHST- 2015/05/01 00:00 [pmc-release] AID - ijms16059504 [pii] AID - ijms-16-09504 [pii] AID - 10.3390/ijms16059504 [doi] PST - epublish SO - Int J Mol Sci. 2015 Apr 27;16(5):9504-19. doi: 10.3390/ijms16059504.