PMID- 25924427
OWN - NLM
STAT- MEDLINE
DCOM- 20150928
LR  - 20211203
IS  - 1672-173X (Print)
IS  - 1672-173X (Linking)
VI  - 46
IP  - 2
DP  - 2015 Mar
TI  - [The study of increased immunogenicity of UCMSC Stimulated by TLR7 agonist 
      CL264].
PG  - 191-6
AB  - OBJECTIVE: To study the change of immune status of umbilical cord mesenchymal 
      stem cells (UCMSC) stimulated by toll like receptor 7 (TLR7) agonist CL264. 
      METHODS: TLR7 specific ligand CL264 was used to stimulate the UCMSC. Flow 
      cytometry was conducted to assay the expression of co-stimulators [human 
      leukocyte antigen (HLA)-E, CD80 and CD86] and surface markers of stem cells 
      (CD29, CD59 and CD90). Quantitative PCR was applied to measure the expression 
      variation of immune-related molecules and stem cell markers. Cell differentiation 
      experiment was used to study the change of differentiation ability of UCMSC upon 
      CL264 stimulation. Peripheral blood mononuclear cells (PBMC) were isolated from 
      healthy human and then cocultured with UCMSC in the presence of CL264. 
      Cytotoxicity assay was used to measure the attack of PBMC to UCMSC. RESULTS: 
      Expression of cotimulatory molecules CD86 and HLA-E were enhanced in UCMSC upon 
      CL264 stimulation. Real-time PCR indicated that many pro-inflammatory molecules 
      [interleukin (IL)-1beta, IL-6, IL-8, IL-10, interferon (IFN)-beta, IFN-gamma, 
      nuclear factor-KB (NF-kappaB), transforming growth factor-beta (TGF-beta)] were 
      induced in the presence of CL264 while the expression of stem cells markers were 
      inhibited [Kruppel-like factor-4 (Klf4), Nestin, SRY-related 
      high-mobility-group-box protein-2 (Sox2), Lin28]. Activation of TLR7 also 
      increased the immune attack of PBMC on UCMSC. Our study also indicated that the 
      treatment of CL264 did not influence the differentiation ability of UCMSC. 
      CONCLUSION: TLR7 agonist CL264 could increase the immunogenicity of UCMSC.
FAU - Yang, Hai-qing
AU  - Yang HQ
FAU - Zhang, Sheng
AU  - Zhang S
FAU - Kuang, Xiao-chuan
AU  - Kuang XC
FAU - Yang, Yin-zhong
AU  - Yang YZ
FAU - Qiu, Fa-qi
AU  - Qiu FQ
LA  - chi
PT  - Journal Article
PL  - China
TA  - Sichuan Da Xue Xue Bao Yi Xue Ban
JT  - Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical 
      science edition
JID - 101162609
RN  - 0 (Cytokines)
RN  - 0 (Immunosuppressive Agents)
RN  - 0 (KLF4 protein, human)
RN  - 0 (Kruppel-Like Factor 4)
RN  - 0 (Kruppel-Like Transcription Factors)
RN  - 0 (NF-kappa B)
RN  - 0 (TLR7 protein, human)
RN  - 0 (Toll-Like Receptor 7)
SB  - IM
MH  - Cell Differentiation
MH  - Coculture Techniques
MH  - Cytokines/metabolism
MH  - Humans
MH  - Immunosuppressive Agents
MH  - Kruppel-Like Factor 4
MH  - Kruppel-Like Transcription Factors/metabolism
MH  - Leukocytes, Mononuclear
MH  - Mesenchymal Stem Cells/*cytology/drug effects
MH  - NF-kappa B/metabolism
MH  - Toll-Like Receptor 7/*agonists
MH  - Umbilical Cord/*cytology
EDAT- 2015/05/01 06:00
MHDA- 2015/09/29 06:00
CRDT- 2015/05/01 06:00
PHST- 2015/05/01 06:00 [entrez]
PHST- 2015/05/01 06:00 [pubmed]
PHST- 2015/09/29 06:00 [medline]
PST - ppublish
SO  - Sichuan Da Xue Xue Bao Yi Xue Ban. 2015 Mar;46(2):191-6.