PMID- 25927246
OWN - NLM
STAT- MEDLINE
DCOM- 20161013
LR  - 20190116
IS  - 1029-2403 (Electronic)
IS  - 1026-8022 (Linking)
VI  - 57
IP  - 1
DP  - 2016
TI  - Features of cell death, mitochondrial activation and caspase dependence of rabbit 
      anti-T-lymphocyte globulin signaling in lymphoblastic Jurkat cells are distinct 
      from classical apoptosis signaling of CD95.
PG  - 177-82
LID - 10.3109/10428194.2015.1044449 [doi]
AB  - Rabbit anti-T-lymphocyte-globulin (ATG) is used for immunosuppression in organ 
      and stem cell transplantation. The aim of this study was to investigate 
      ATG-induced cell death compared to CD95-signaling of apoptosis. We measured 
      features of cell death at the cell membrane, mitochondria, nuclei and caspase-3 
      cleavage. We used the following inhibitors: the caspase inhibitor 
      N-benzyloxycarbonyl-Val-Ala-Asp (O-Me)-fluoromethyl ketone (zVAD-fmk), the serine 
      protease inhibitors 3,4 dichloroisocoumarin (DCI) and 
      N-alpha-tosyl-L-lysinyl-chloromethylketone (TLCK) and the reducing agent 
      N-acetycysteine (NAC). ATG-induced cellular changes were rapid, included 
      mitochondrial membrane permeability (MMP) induction and annexin V/propidium 
      iodide (PI) positivity but little caspase-3 activation and nuclear morphology 
      changes. MMP was not sensitive to caspase inhibition, serine protease inhibition 
      with DCI moderately reduced MMP. These findings were in contrast to 
      CD95-signaling. Interestingly, TLCK massively augmented CD95-induced MMP which 
      could be abrogated by NAC. In conclusion, ATG-signaling differs in features and 
      kinetics from CD95-induced apoptosis with caspase-independent mechanisms involved 
      in MMP.
FAU - Ziegler, Christian
AU  - Ziegler C
AD  - a Department of Medical Oncology , National Center for Tumor Diseases, Heidelberg 
      University Hospital , Heidelberg , Germany.
AD  - b Department of Hematology and Oncology , Albert Ludwigs-University Medical 
      Center Freiburg , Freiburg , Germany.
AD  - c Department of Hematology and Oncology , Charite Medical Center Berlin , Campus 
      Virchow Klinik, Berlin, Germany.
FAU - Finke, Jurgen
AU  - Finke J
AD  - b Department of Hematology and Oncology , Albert Ludwigs-University Medical 
      Center Freiburg , Freiburg , Germany.
FAU - Grullich, Carsten
AU  - Grullich C
AD  - a Department of Medical Oncology , National Center for Tumor Diseases, Heidelberg 
      University Hospital , Heidelberg , Germany.
AD  - b Department of Hematology and Oncology , Albert Ludwigs-University Medical 
      Center Freiburg , Freiburg , Germany.
LA  - eng
PT  - Journal Article
DEP - 20150525
PL  - United States
TA  - Leuk Lymphoma
JT  - Leukemia & lymphoma
JID - 9007422
RN  - 0 (Antilymphocyte Serum)
RN  - 0 (fas Receptor)
RN  - EC 3.4.22.- (Caspase 3)
RN  - EC 3.4.22.- (Caspases)
RN  - EC 3.4.24.- (Matrix Metalloproteinases)
SB  - IM
MH  - Animals
MH  - Antilymphocyte Serum/*pharmacology
MH  - Apoptosis/*drug effects
MH  - Caspase 3/metabolism
MH  - Caspases/*metabolism
MH  - Cell Membrane Permeability
MH  - Humans
MH  - Jurkat Cells
MH  - Matrix Metalloproteinases/metabolism
MH  - Mitochondria/*drug effects/*metabolism
MH  - Mitochondrial Membranes/metabolism
MH  - Rabbits
MH  - Signal Transduction/*drug effects
MH  - fas Receptor/*metabolism
OTO - NOTNLM
OT  - CD95
OT  - Caspase
OT  - Jurkat
OT  - rabbit anti-T-lymphocyte-globulin (ATG)
OT  - reactive oxygen species (ROS)
OT  - serine protease
EDAT- 2015/05/01 06:00
MHDA- 2016/10/14 06:00
CRDT- 2015/05/01 06:00
PHST- 2015/05/01 06:00 [entrez]
PHST- 2015/05/01 06:00 [pubmed]
PHST- 2016/10/14 06:00 [medline]
AID - 10.3109/10428194.2015.1044449 [doi]
PST - ppublish
SO  - Leuk Lymphoma. 2016;57(1):177-82. doi: 10.3109/10428194.2015.1044449. Epub 2015 
      May 25.