PMID- 25929435
OWN - NLM
STAT- MEDLINE
DCOM- 20160203
LR  - 20181113
IS  - 1420-908X (Electronic)
IS  - 1023-3830 (Linking)
VI  - 64
IP  - 6
DP  - 2015 Jun
TI  - Higenamine regulates Nrf2-HO-1-Hmgb1 axis and attenuates intestinal 
      ischemia-reperfusion injury in mice.
PG  - 395-403
LID - 10.1007/s00011-015-0817-x [doi]
AB  - INTRODUCTION: Intestinal ischemia and reperfusion (IR) syndrome is a 
      life-threatening dilemma caused by diverse events. Higenamine (HG), an active 
      ingredient of Aconiti Lateralis Radix Praeparata, has been traditionally used as 
      a heart stimulant and anti-inflammatory agent in oriental countries. But the 
      function of HG on intestine IR injury has never been investigated. MATERIALS AND 
      METHODS: Mice underwent a 2 cm midline laparotomy, and the superior mesenteric 
      artery (SMA) was obstructed by micro-vascular clamp to induce intestinal 
      ischemia. RESULTS: In our current study, HG increases mouse intestinal epithelial 
      (IEC-6) cell viability through induced heme oxygenase-1 (HO-1) production in 
      vitro. In our in vivo murine intestinal IR injury model, the increased HO-1 
      protein level and activity, decreased intestinal injury score, Myeloperoxidase 
      (MPO) activity, and inflammatory cytokine expression induced by HG were all 
      abolished with additional treatment of HO-1 inhibitor zinc protoporphyrin IX 
      (ZnPPIX). Furthermore, HG reduced high mobility group box-1 (Hmgb1) expression in 
      IR injury-performed intestine which was inhibited by additional administration of 
      ZnPPIX. And HG treatment significantly decreased HO-1 expression in nuclear 
      factor erythroid 2-related factor (Nrf-2) SiRNA-transfected cells but not in 
      control SiRNA-transfected cells. CONCLUSION: Our study provides evidence HG 
      regulates Nrf2-HO-1-Hmgb1 axis and attenuates intestinal IR injury in mice.
FAU - Liu, Chao
AU  - Liu C
AD  - Department of General Surgery, Yichang Central People's Hospital, The First 
      College of Clinical Medical Science, China Three Gorges University, No.183, 
      Yiling Road, Yichang, 443003, Hubei, China.
FAU - Zhu, Chenyu
AU  - Zhu C
FAU - Wang, Guangsheng
AU  - Wang G
FAU - Xu, Rui
AU  - Xu R
FAU - Zhu, Yaoming
AU  - Zhu Y
LA  - eng
PT  - Journal Article
DEP - 20150501
PL  - Switzerland
TA  - Inflamm Res
JT  - Inflammation research : official journal of the European Histamine Research 
      Society ... [et al.]
JID - 9508160
RN  - 0 (Alkaloids)
RN  - 0 (Anti-Inflammatory Agents, Non-Steroidal)
RN  - 0 (HMGB1 Protein)
RN  - 0 (HMGB1 protein, mouse)
RN  - 0 (NF-E2-Related Factor 2)
RN  - 0 (Nfe2l2 protein, mouse)
RN  - 0 (Protoporphyrins)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Tetrahydroisoquinolines)
RN  - 15442-64-5 (zinc protoporphyrin)
RN  - EC 1.11.1.7 (Peroxidase)
RN  - EC 1.14.14.18 (Heme Oxygenase-1)
RN  - TBV5O16GAP (higenamine)
SB  - IM
MH  - Alkaloids/*pharmacology/therapeutic use
MH  - Animals
MH  - Anti-Inflammatory Agents, Non-Steroidal/*pharmacology/therapeutic use
MH  - Epithelial Cells/drug effects
MH  - HMGB1 Protein/*drug effects/*metabolism
MH  - Heme Oxygenase-1/antagonists & inhibitors/*drug effects/*metabolism
MH  - Intestinal Diseases/*drug therapy
MH  - Laparotomy
MH  - Male
MH  - Mesenteric Artery, Superior/drug effects
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - NF-E2-Related Factor 2/*drug effects/*metabolism
MH  - Peroxidase/metabolism
MH  - Protoporphyrins/pharmacology
MH  - RNA, Small Interfering/genetics
MH  - Reperfusion Injury/*drug therapy
MH  - Tetrahydroisoquinolines/*pharmacology/therapeutic use
MH  - Transfection
EDAT- 2015/05/02 06:00
MHDA- 2016/02/04 06:00
CRDT- 2015/05/02 06:00
PHST- 2014/08/03 00:00 [received]
PHST- 2015/04/02 00:00 [accepted]
PHST- 2015/03/12 00:00 [revised]
PHST- 2015/05/02 06:00 [entrez]
PHST- 2015/05/02 06:00 [pubmed]
PHST- 2016/02/04 06:00 [medline]
AID - 10.1007/s00011-015-0817-x [doi]
PST - ppublish
SO  - Inflamm Res. 2015 Jun;64(6):395-403. doi: 10.1007/s00011-015-0817-x. Epub 2015 
      May 1.