PMID- 25929832
OWN - NLM
STAT- MEDLINE
DCOM- 20160314
LR  - 20181113
IS  - 1559-1166 (Electronic)
IS  - 0895-8696 (Linking)
VI  - 56
IP  - 2
DP  - 2015 Jun
TI  - Neuroprotective Effects of Rutin in Streptozotocin-Induced Diabetic Rat Retina.
PG  - 440-8
LID - 10.1007/s12031-015-0561-2 [doi]
AB  - Diabetic retinopathy is widely recognized as a neurodegenerative disease of the 
      eye. Increased oxidative stress has been considered the central factor in 
      damaging neural retina in diabetes. Flavonoids, being powerful antioxidants, play 
      protective roles in several oxidative stress-mediated neurodegenerative diseases. 
      In this study, we analyzed the neuroprotective effects of a potential flavonoid, 
      rutin, in the diabetic rat retina. Diabetes was induced in male Wistar rats by 
      single injection of streptozotocin (65 mg/kg). In age-matched control 
      (non-diabetic) and 1 week of diabetic rats, rutin (100 mg/kg/day) was orally 
      administered and continued for 5 weeks. In another group of diabetic rats, only 
      saline was supplemented. After treatments, retinas from all the groups were 
      isolated and analyzed for potential neurotrophic factors and apoptotic and 
      oxidative stress markers using biochemical and immunoblotting techniques. Our 
      results indicate that rutin possesses antidiabetic activity, as blood glucose 
      level decreased and insulin level increased in diabetic rats. In the diabetic 
      retina, rutin supplementation enhanced the reduced levels of brain-derived 
      neurotrophic factor (BDNF), nerve growth factor (NGF), and glutathione (GSH) (P < 
      0.05), and reduced the level of thiobarbituric acid-reactive substances (TBARS) 
      (P < 0.05). In addition, rutin treatment showed antiapoptotic activity by 
      decreasing the level of caspase-3 and increasing the level of Bcl-2 in the 
      diabetic retina. These results suggest the effectiveness of rutin in ameliorating 
      the levels of neuroprotective factors in diabetic retina. Therefore, rutin might 
      be a potential flavonoid that can prevent the retinal damage and subsequently the 
      development of diabetic retinopathy.
FAU - Ola, Mohammad Shamsul
AU  - Ola MS
AD  - Department of Biochemistry, College of Science, King Saud University, Riyadh, 
      11415, Saudi Arabia, mola@ksu.edu.sa.
FAU - Ahmed, Mohammed M
AU  - Ahmed MM
FAU - Ahmad, Rehan
AU  - Ahmad R
FAU - Abuohashish, Hatem M
AU  - Abuohashish HM
FAU - Al-Rejaie, Salim S
AU  - Al-Rejaie SS
FAU - Alhomida, Abdullah S
AU  - Alhomida AS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150501
PL  - United States
TA  - J Mol Neurosci
JT  - Journal of molecular neuroscience : MN
JID - 9002991
RN  - 0 (Blood Glucose)
RN  - 0 (Hypoglycemic Agents)
RN  - 0 (Insulin)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Neuroprotective Agents)
RN  - 5G06TVY3R7 (Rutin)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Blood Glucose/metabolism
MH  - Diabetes Mellitus, Experimental/*drug therapy
MH  - Diabetic Retinopathy/*drug therapy
MH  - Hypoglycemic Agents/pharmacology/*therapeutic use
MH  - Insulin/metabolism
MH  - Nerve Growth Factors/metabolism
MH  - Neuroprotective Agents/pharmacology/*therapeutic use
MH  - Oxidative Stress
MH  - Retina/drug effects/metabolism
MH  - Rutin/pharmacology/*therapeutic use
EDAT- 2015/05/02 06:00
MHDA- 2016/03/15 06:00
CRDT- 2015/05/02 06:00
PHST- 2015/01/28 00:00 [received]
PHST- 2015/03/31 00:00 [accepted]
PHST- 2015/05/02 06:00 [entrez]
PHST- 2015/05/02 06:00 [pubmed]
PHST- 2016/03/15 06:00 [medline]
AID - 10.1007/s12031-015-0561-2 [doi]
PST - ppublish
SO  - J Mol Neurosci. 2015 Jun;56(2):440-8. doi: 10.1007/s12031-015-0561-2. Epub 2015 
      May 1.