PMID- 25930987
OWN - NLM
STAT- MEDLINE
DCOM- 20160325
LR  - 20220408
IS  - 1879-3150 (Electronic)
IS  - 0041-0101 (Linking)
VI  - 101
DP  - 2015 Jul
TI  - Homology modeling, vasorelaxant and bradykinin-potentiating activities of a novel 
      hypotensin found in the scorpion venom from Tityus stigmurus.
PG  - 11-8
LID - S0041-0101(15)00100-2 [pii]
LID - 10.1016/j.toxicon.2015.04.003 [doi]
AB  - In a recent work by our group involving a transcriptomics approach applied to the 
      venom glands from Tityus stigmurus we identified a new family of peptides called 
      Hypotensins (TSTI0006C) (Almeida et al., 2012). The cluster TSTI0006C was 
      analyzed in the main 25 amino acid residues and named T. stigmurus Hypotensin 
      (TistH), showing a molecular mass of 2.7 kDa, an absence of cysteines and the 
      presence of two C-terminal proline residues, which are a bradykinin-potentiating 
      peptide (BPP) signature. Here, we describe the homology modeling of the 
      three-dimensional structure of TistH. In addition, we evaluated the 
      cardiovascular effects elicited by TistH in normotensive rats. Firstly, TistH 
      showed no cytotoxic effect on horse erythrocyte. Furthermore, in normotensive 
      rats TistH was able to potentiate the hypotensive action of bradykinin (BK) and 
      induced a vasorelaxant effect in mesenteric artery rings by endothelium-dependent 
      release of nitric oxide (NO) and demonstrated independent inhibition of 
      angiotensin converting enzyme (ACE). Our data can contribute to a better 
      understanding of the structural and functional characteristics of TistH and 
      suggest its potential use in cardiovascular diseases.
CI  - Copyright (c) 2015 Elsevier Ltd. All rights reserved.
FAU - Machado, Richele J A
AU  - Machado RJ
AD  - Laboratorio de Tecnologia e Biotecnologia Farmaceutica, Universidade Federal do 
      Rio Grande do Norte, Natal, RN, Brazil; Programa de Pos-Graduacao em Bioquimica, 
      Universidade Federal do Rio Grande do Norte, Natal, RN, Brazil.
FAU - Junior, Leonidas G M
AU  - Junior LG
AD  - Departamento de Biotecnologia da Universidade Federal da Paraiba, Joao Pessoa, 
      PB, Brazil.
FAU - Monteiro, Norberto K V
AU  - Monteiro NK
AD  - Instituto de Quimica, Universidade Federal do Rio Grande do Norte, Natal, RN, 
      Brazil.
FAU - Silva-Junior, Arnobio A
AU  - Silva-Junior AA
AD  - Laboratorio de Tecnologia e Biotecnologia Farmaceutica, Universidade Federal do 
      Rio Grande do Norte, Natal, RN, Brazil.
FAU - Portaro, Fernanda C V
AU  - Portaro FC
AD  - Laboratorio de Imunoquimica, Instituto Butantan, Sao Paulo, SP, Brazil.
FAU - Barbosa, Euzebio G
AU  - Barbosa EG
AD  - Laboratorio de Quimica Farmaceutica, Universidade Federal do Rio Grande do Norte, 
      Natal, RN, Brazil.
FAU - Braga, Valdir A
AU  - Braga VA
AD  - Departamento de Biotecnologia da Universidade Federal da Paraiba, Joao Pessoa, 
      PB, Brazil.
FAU - Fernandes-Pedrosa, Matheus F
AU  - Fernandes-Pedrosa MF
AD  - Laboratorio de Tecnologia e Biotecnologia Farmaceutica, Universidade Federal do 
      Rio Grande do Norte, Natal, RN, Brazil; Programa de Pos-Graduacao em Bioquimica, 
      Universidade Federal do Rio Grande do Norte, Natal, RN, Brazil. Electronic 
      address: mpedrosa@ufrnet.br.
LA  - eng
PT  - Journal Article
DEP - 20150428
PL  - England
TA  - Toxicon
JT  - Toxicon : official journal of the International Society on Toxinology
JID - 1307333
RN  - 0 (Angiotensin-Converting Enzyme Inhibitors)
RN  - 0 (Antihypertensive Agents)
RN  - 0 (Scorpion Venoms)
RN  - 0 (Vasodilator Agents)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - EC 3.4.15.1 (Peptidyl-Dipeptidase A)
RN  - S8TIM42R2W (Bradykinin)
SB  - IM
MH  - Angiotensin-Converting Enzyme Inhibitors/chemistry/pharmacology
MH  - Animals
MH  - Antihypertensive Agents/pharmacology
MH  - Blood Pressure/drug effects
MH  - Bradykinin/chemistry/*pharmacology
MH  - Cardiovascular System/drug effects/metabolism
MH  - Cloning, Molecular
MH  - Computational Biology
MH  - Models, Molecular
MH  - Nitric Oxide/metabolism
MH  - Peptidyl-Dipeptidase A/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Scorpion Venoms/chemistry/*pharmacology
MH  - Scorpions/*metabolism
MH  - Transcriptome
MH  - Vasodilator Agents/chemistry/*pharmacology
OTO - NOTNLM
OT  - Anti-hypertensive peptides
OT  - Bradykinin-potentiating peptides
OT  - Hypotensin
OT  - Nitric oxide
OT  - Tityus stigmurus
EDAT- 2015/05/02 06:00
MHDA- 2016/03/26 06:00
CRDT- 2015/05/02 06:00
PHST- 2014/12/08 00:00 [received]
PHST- 2015/03/18 00:00 [revised]
PHST- 2015/04/21 00:00 [accepted]
PHST- 2015/05/02 06:00 [entrez]
PHST- 2015/05/02 06:00 [pubmed]
PHST- 2016/03/26 06:00 [medline]
AID - S0041-0101(15)00100-2 [pii]
AID - 10.1016/j.toxicon.2015.04.003 [doi]
PST - ppublish
SO  - Toxicon. 2015 Jul;101:11-8. doi: 10.1016/j.toxicon.2015.04.003. Epub 2015 Apr 28.