PMID- 25934483 OWN - NLM STAT- MEDLINE DCOM- 20160317 LR - 20220410 IS - 1471-2466 (Electronic) IS - 1471-2466 (Linking) VI - 15 DP - 2015 May 2 TI - Oxidative stress and nitric oxide signaling related biomarkers in patients with pulmonary hypertension: a case control study. PG - 50 LID - 10.1186/s12890-015-0045-8 [doi] LID - 50 AB - BACKGROUND: Oxidative stress (OS) and reduced nitric oxide (NO) bioavailability contribute to the pathogenesis of pulmonary hypertension (PH). Whether there are associations between OS and NO signaling biomarkers and whether these biomarkers are associated with the severity of PH remain unclear. METHODS: Blood samples were collected from 35 healthy controls and 35 patients with pulmonary arterial hypertension (PAH, n = 12) or chronic thromboembolic pulmonary hypertension (CTEPH, n = 23). The mean pulmonary artery pressure (mPAP) and pulmonary vascular resistance index (PVRI) were measured by right heart catheterization. We measured the derivative of reactive oxygen molecules (d-ROMs), biological antioxidant potential (BAP) and superoxide dismutase (SOD) by automatic biochemical analyzer, malondialdehyde (MDA) and asymmetric dimethylarginine (ADMA) by enzyme-linked immunosorbent assay. The relationship between oxidative-antioxidative biomarkers and ADMA, as well as their association with pulmonary hemodynamics, were analyzed. RESULTS: Compared with age- and gender-matched controls, there was no significant difference of d-ROMs in PAH and CTEPH patients; MDA was increased in CTEPH patients (P = 0.034); BAP and SOD were decreased in PAH (P = 0.014, P < 0.001) and CTEPH patients (P = 0.015, P < 0.001); ADMA level was significantly higher in PAH (P = 0.007) and CTEPH patients (P < 0.001). No association between oxidative-antioxidative biomarkers and ADMA was found. Serum ADMA concentration was correlated with mPAP (r = 0.762, P = 0.006) and PVRI (r = 0.603, P = 0.038) in PAH patients. CONCLUSIONS: The antioxidative potential and NO signaling are impaired in PAH and CTEPH. Increased serum ADMA level is associated with unfavorable pulmonary hemodynamics in PAH patients. Thus, ADMA may be useful in the severity evaluation and risk stratification of PAH. FAU - Zhang, Shuai AU - Zhang S AD - Department of Pulmonary and Critical Care Medicine, Beijing Institute of Respiratory Medicine, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, People's Republic of China. shuaizhang2012@126.com. AD - Beijing Key Laboratory of Respiratory and Pulmonary Circulation Disorders, Beijing, People's Republic of China. shuaizhang2012@126.com. FAU - Yang, Ting AU - Yang T AD - Department of Pulmonary and Critical Care Medicine, Beijing Institute of Respiratory Medicine, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, People's Republic of China. dryangting@qq.com. AD - Beijing Key Laboratory of Respiratory and Pulmonary Circulation Disorders, Beijing, People's Republic of China. dryangting@qq.com. FAU - Xu, Xiaomao AU - Xu X AD - Department of Pulmonary and Critical Care Medicine, Beijing Hospital, Beijing, People's Republic of China. xuxiaomao@163.com. AD - National Clinical Research Center of Respiratory Diseases, Beijing, People's Republic of China. xuxiaomao@163.com. FAU - Wang, Meng AU - Wang M AD - Department of Laboratory Medicine, Beijing Hospital, Beijing, People's Republic of China. wangmenglj1@vip.tom.com. FAU - Zhong, Linye AU - Zhong L AD - Department of Pulmonary and Critical Care Medicine, Beijing Institute of Respiratory Medicine, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, People's Republic of China. zhonglinye756@163.com. AD - Beijing Key Laboratory of Respiratory and Pulmonary Circulation Disorders, Beijing, People's Republic of China. zhonglinye756@163.com. FAU - Yang, Yuanhua AU - Yang Y AD - Department of Pulmonary and Critical Care Medicine, Beijing Institute of Respiratory Medicine, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, People's Republic of China. yyh1031@sina.com. AD - Beijing Key Laboratory of Respiratory and Pulmonary Circulation Disorders, Beijing, People's Republic of China. yyh1031@sina.com. FAU - Zhai, Zhenguo AU - Zhai Z AD - Department of Pulmonary and Critical Care Medicine, Beijing Institute of Respiratory Medicine, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, People's Republic of China. zhaizhenguo2011@126.com. AD - Beijing Key Laboratory of Respiratory and Pulmonary Circulation Disorders, Beijing, People's Republic of China. zhaizhenguo2011@126.com. FAU - Xiao, Fei AU - Xiao F AD - National Clinical Research Center of Respiratory Diseases, Beijing, People's Republic of China. xiaofei@bjhmoh.cn. AD - Department of Cell Biology, Institute of Geriatrics, Beijing Hospital, Beijing, People's Republic of China. xiaofei@bjhmoh.cn. FAU - Wang, Chen AU - Wang C AD - National Clinical Research Center of Respiratory Diseases, Beijing, People's Republic of China. cyh-birm@263.net. AD - Department of Respiratory Medicine, Capital Medical University, Beijing, People's Republic of China. cyh-birm@263.net. AD - China-Japan Friendship Hospital, Beijing, People's Republic of China. cyh-birm@263.net. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20150502 PL - England TA - BMC Pulm Med JT - BMC pulmonary medicine JID - 100968563 RN - 0 (Biomarkers) RN - 31C4KY9ESH (Nitric Oxide) SB - IM MH - Adult MH - Biomarkers/*blood MH - Enzyme-Linked Immunosorbent Assay MH - Female MH - Humans MH - Hypertension, Pulmonary/*metabolism/physiopathology MH - Male MH - Middle Aged MH - Nitric Oxide/*metabolism MH - *Oxidative Stress MH - Pulmonary Wedge Pressure/*physiology MH - Signal Transduction MH - Vascular Resistance/physiology PMC - PMC4477508 EDAT- 2015/05/03 06:00 MHDA- 2016/03/18 06:00 PMCR- 2015/05/02 CRDT- 2015/05/03 06:00 PHST- 2014/11/24 00:00 [received] PHST- 2015/04/22 00:00 [accepted] PHST- 2015/05/03 06:00 [entrez] PHST- 2015/05/03 06:00 [pubmed] PHST- 2016/03/18 06:00 [medline] PHST- 2015/05/02 00:00 [pmc-release] AID - 10.1186/s12890-015-0045-8 [pii] AID - 45 [pii] AID - 10.1186/s12890-015-0045-8 [doi] PST - epublish SO - BMC Pulm Med. 2015 May 2;15:50. doi: 10.1186/s12890-015-0045-8.