PMID- 25935536
OWN - NLM
STAT- MEDLINE
DCOM- 20160128
LR  - 20221207
IS  - 1423-0380 (Electronic)
IS  - 1010-4283 (Linking)
VI  - 36
IP  - 10
DP  - 2015 Sep
TI  - Sex-determining region Y-related high mobility group box (SOX)-2 is overexpressed 
      in cervical squamous cell carcinoma and contributes cervical cancer cell 
      migration and invasion in vitro.
PG  - 7725-33
LID - 10.1007/s13277-015-3450-y [doi]
AB  - Sex-determining region Y-related high mobility group box 2 (SOX-2) is a key 
      pluripotency-associated transcription factor and may be implicated in the 
      pathogenesis of cervical squamous cell carcinoma (SCC). The aim of this study was 
      to explore SOX-2 expression in cervical SCC tissues and to examine whether and 
      how SOX-2 regulates the malignant behaviors of cervical SCC cells in vitro. We 
      here found that SOX-2 expression in the examined cervical SCC tissues was higher 
      than that in the normal cervical and cervical intraepithelial neoplasia (CIN) 
      tissues. Higher protein level of SOX-2 (nuclear positive staining cells >/=50 %) 
      was detected in 34.9 % (29 out of 83 cases) of cervical SCC patients. We also 
      noted that 100 % of well-differentiated and 66.7 % of moderately differentiated 
      cervical SCCs showed lower SOX-2 expression (nuclear positive staining cells <50 
      %), while 58.8 % of poorly differentiated tumors had higher SOX-2 expression 
      (P < 0.05). Furthermore, the migratory and invasive capabilities of SiHa cervical 
      cancer cells were enhanced when SOX-2 was upregulated whereas suppressed when 
      SOX-2 was downregulated. Also, the phosphorylation levels of protein kinase B 
      (Akt) and extracellular regulated protein kinases (ERK) 1/2 were increased in 
      SOX-2-overexpressed cancer cells but decreased in SOX-2-depleted cells. 
      Additionally, LY294002 (Akt pathway inhibitor) or U0126 (ERK pathway inhibitor) 
      significantly suppressed SOX-2-overexpression-induced migration and invasion in 
      SiHa cells. Our results indicate that differentially expressed SOX-2 is 
      associated with tumor differentiation (P < 0.05) and that SOX-2 contributes to 
      the migratory and invasive behaviors of cervical SCC in vitro.
FAU - Chang, Xiaohan
AU  - Chang X
AD  - Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical 
      University, 36 Sanhao Street, Shenyang, 110004, People's Republic of China.
FAU - Zhang, Jing
AU  - Zhang J
AD  - Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical 
      University, 36 Sanhao Street, Shenyang, 110004, People's Republic of China.
FAU - Huang, Chenglin
AU  - Huang C
AD  - Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical 
      University, 36 Sanhao Street, Shenyang, 110004, People's Republic of China.
FAU - Pang, Xiaoao
AU  - Pang X
AD  - Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical 
      University, 36 Sanhao Street, Shenyang, 110004, People's Republic of China.
FAU - Luo, Qingshuang
AU  - Luo Q
AD  - Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical 
      University, 36 Sanhao Street, Shenyang, 110004, People's Republic of China.
FAU - Zhang, Huijie
AU  - Zhang H
AD  - Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical 
      University, 36 Sanhao Street, Shenyang, 110004, People's Republic of China.
FAU - Zhang, Shulan
AU  - Zhang S
AD  - Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical 
      University, 36 Sanhao Street, Shenyang, 110004, People's Republic of China. 
      zsl0909@sina.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150503
PL  - Netherlands
TA  - Tumour Biol
JT  - Tumour biology : the journal of the International Society for Oncodevelopmental 
      Biology and Medicine
JID - 8409922
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (RNA, Messenger)
RN  - 0 (SOX2 protein, human)
RN  - 0 (SOXB1 Transcription Factors)
SB  - IM
MH  - Apoptosis
MH  - Biomarkers, Tumor/genetics/*metabolism
MH  - Blotting, Western
MH  - Carcinoma, Squamous Cell/genetics/metabolism/*pathology
MH  - *Cell Movement
MH  - Cell Proliferation
MH  - Female
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - Middle Aged
MH  - Neoplasm Grading
MH  - Neoplasm Invasiveness
MH  - Prognosis
MH  - RNA, Messenger/genetics
MH  - Real-Time Polymerase Chain Reaction
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - SOXB1 Transcription Factors/genetics/*metabolism
MH  - Tumor Cells, Cultured
MH  - Uterine Cervical Neoplasms/genetics/metabolism/*pathology
MH  - Wound Healing
MH  - Uterine Cervical Dysplasia/genetics/metabolism/*pathology
OTO - NOTNLM
OT  - Cervical squamous cell carcinoma
OT  - Clinicopathologic features
OT  - Invasion
OT  - Migration
OT  - Sex-determining region Y-related high mobility group box 2
EDAT- 2015/05/04 06:00
MHDA- 2016/01/29 06:00
CRDT- 2015/05/04 06:00
PHST- 2014/10/20 00:00 [received]
PHST- 2015/04/10 00:00 [accepted]
PHST- 2015/05/04 06:00 [entrez]
PHST- 2015/05/04 06:00 [pubmed]
PHST- 2016/01/29 06:00 [medline]
AID - 10.1007/s13277-015-3450-y [pii]
AID - 10.1007/s13277-015-3450-y [doi]
PST - ppublish
SO  - Tumour Biol. 2015 Sep;36(10):7725-33. doi: 10.1007/s13277-015-3450-y. Epub 2015 
      May 3.