PMID- 25935837
OWN - NLM
STAT- MEDLINE
DCOM- 20160308
LR  - 20220408
IS  - 1745-7270 (Electronic)
IS  - 1672-9145 (Linking)
VI  - 47
IP  - 6
DP  - 2015 Jun
TI  - miR-330-3p controls cell proliferation by targeting early growth response 2 in 
      non-small-cell lung cancer.
PG  - 431-40
LID - 10.1093/abbs/gmv032 [doi]
AB  - Non-small-cell lung cancer (NSCLC) is one of the most common lung cancers, and 
      microRNAs (miRNAs) have been reported to play essential roles in NSCLC. Recent 
      studies have indicated that miR-330-3p expression is up-regulated in NSCLC 
      samples and in tissues of NSCLC brain metastasis. In this study, up-regulation of 
      miR-330-3p expression was confirmed in NSCLC and 20 NSCLC patient samples. 
      Furthermore, miR-330-3p was over-expressed in NSCLC cell lines A549 and H23, and 
      the promotive function of miR-330-3p was investigated in regulating NSCLC cell 
      proliferation and cell cycle distribution. To identify potential target genes of 
      miR-330-3p in NSCLC, the miRNA target prediction databases were used. Luciferase 
      activity assay and real-time RT-PCR analysis confirmed that miR-330-3p is 
      negatively correlated with the expression of early growth response 2 (EGR2). 
      Moreover, it was also found that EGR2 mRNA contains two potential binding sites 
      for miR-330-3p. Knock-down of EGR2 with siRNA was demonstrated to have a similar 
      effect as the over-expression of miR-330-3p in NSCLC cell lines. Taken together, 
      our results show that EGR2 is a target of miR-330-3p.
CI  - (c) The Author 2015. Published by ABBS Editorial Office in association with Oxford 
      University Press on behalf of the Institute of Biochemistry and Cell Biology, 
      Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences.
FAU - Liu, Xuzhi
AU  - Liu X
AD  - Department of Respiratory Medicine, the Third Affiliated Hospital of Qiqihar 
      Medical University, Qiqihar 161000, China.
FAU - Shi, Hanbing
AU  - Shi H
AD  - Department of Respiratory Medicine, the Third Affiliated Hospital of Qiqihar 
      Medical University, Qiqihar 161000, China.
FAU - Liu, Bo
AU  - Liu B
AD  - Department of Respiratory Medicine, the Third Affiliated Hospital of Qiqihar 
      Medical University, Qiqihar 161000, China.
FAU - Li, Jianing
AU  - Li J
AD  - Department of Respiratory Medicine, the Second Affiliated Hospital of Harbin 
      Medical University, Harbin 150086, China.
FAU - Liu, Yaxin
AU  - Liu Y
AD  - Department of Respiratory Medicine, the Second Affiliated Hospital of Harbin 
      Medical University, Harbin 150086, China.
FAU - Yu, Baiquan
AU  - Yu B
AD  - Department of Respiratory Medicine, the Second Affiliated Hospital of Harbin 
      Medical University, Harbin 150086, China yubaiquan76@163.com.
LA  - eng
PT  - Journal Article
DEP - 20150502
PL  - China
TA  - Acta Biochim Biophys Sin (Shanghai)
JT  - Acta biochimica et biophysica Sinica
JID - 101206716
RN  - 0 (EGR2 protein, human)
RN  - 0 (Early Growth Response Protein 2)
RN  - 0 (MIRN330 microRNA, human)
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Carcinoma, Non-Small-Cell Lung/metabolism/*pathology
MH  - Cell Line, Tumor
MH  - Cell Proliferation/*physiology
MH  - Early Growth Response Protein 2/*metabolism
MH  - Humans
MH  - Lung Neoplasms/metabolism/*pathology
MH  - MicroRNAs/metabolism/*physiology
MH  - Up-Regulation
OTO - NOTNLM
OT  - EGR2
OT  - miR-330-3p
OT  - non-small-cell lung cancer
OT  - proliferation
EDAT- 2015/05/04 06:00
MHDA- 2016/03/10 06:00
CRDT- 2015/05/04 06:00
PHST- 2014/12/17 00:00 [received]
PHST- 2015/02/26 00:00 [accepted]
PHST- 2015/05/04 06:00 [entrez]
PHST- 2015/05/04 06:00 [pubmed]
PHST- 2016/03/10 06:00 [medline]
AID - gmv032 [pii]
AID - 10.1093/abbs/gmv032 [doi]
PST - ppublish
SO  - Acta Biochim Biophys Sin (Shanghai). 2015 Jun;47(6):431-40. doi: 
      10.1093/abbs/gmv032. Epub 2015 May 2.