PMID- 25937838
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20150504
LR  - 20220321
IS  - 1758-5996 (Print)
IS  - 1758-5996 (Electronic)
IS  - 1758-5996 (Linking)
VI  - 6
DP  - 2014
TI  - Plasma and urine metabolic profiles are reflective of altered beta-oxidation in 
      non-diabetic obese subjects and patients with type 2 diabetes mellitus.
PG  - 129
LID - 10.1186/1758-5996-6-129 [doi]
LID - 129
AB  - OBJECTIVES: The two primary pathophysiological characteristics of patients with 
      type 2 diabetes mellitus (T2DM) are insulin resistance (IR) and beta cell 
      dysfunction. It has been proposed that the development of IR is secondary to the 
      accumulation of triacylglycerols and fatty acids in the muscle and liver, which 
      is in turn thought to be secondary to an enzymatic defect in mitochondrial 
      beta-oxidation. The purpose of the present study was to analyze the molecules of 
      intermediary metabolism to determine if an alteration in mitochondrial function 
      exists in T2DM patients and, if so, to determine whether this alteration is 
      caused by excess nutrients or an enzymatic defect. DESIGN AND METHODS: 
      Seventy-seven subjects were recruited and divided into four groups (21 T2DM 
      patients, 17 non-diabetic overweight/obese subjects, 20 offspring of T2DM 
      patients, and 19 healthy subjects). Anthropometric parameters were determined by 
      air plethysmography, and biochemical and metabolic parameters were measured, 
      including 31 acylcarnitines (ACs) and 13 amino acids quantified by MS/MS and 67 
      organic acids measured by GC/MS. RESULTS: Patients with T2DM showed elevation of 
      short-chain ACs (C2, C4), a glycogenic amino acid (valine), a glycogenic and 
      ketogenic amino acid (tyrosine), and a ketogenic amino acid (leucine) as well as 
      altered excretion of dicarboxylic acids. T2DM offspring with abnormal glucose 
      tolerance test GTT showed increased levels of C16. Subjects in the obese group 
      who were dysglycemic also showed altered urinary excretion of dicarboxylic acids 
      and lower levels of a long-chain AC (C14:2). CONCLUSIONS: These results suggest 
      that mitochondrial beta-oxidation is altered in T2DM patients and that the 
      alteration is most likely caused by nutrient overload through a different pathway 
      from that observed in obese subjects.
FAU - Villarreal-Perez, Jesus Zacarias
AU  - Villarreal-Perez JZ
AD  - Universidad Autonoma de Nuevo Leon, Hospital Universitario, "Dr. Jose Eleuterio 
      Gonzalez", Servicio de Endocrinologia, Monterrey, Nuevo Leon, 64460 Mexico.
FAU - Villarreal-Martinez, Jesus Zacarias
AU  - Villarreal-Martinez JZ
AD  - Departamento de Medicina Interna, Universidad Autonoma de Nuevo Leon, Hospital 
      Universitario, "Dr. Jose Eleuterio Gonzalez", Monterrey, Nuevo Leon 64460 Mexico.
FAU - Lavalle-Gonzalez, Fernando Javier
AU  - Lavalle-Gonzalez FJ
AD  - Universidad Autonoma de Nuevo Leon, Hospital Universitario, "Dr. Jose Eleuterio 
      Gonzalez", Servicio de Endocrinologia, Monterrey, Nuevo Leon, 64460 Mexico.
FAU - Torres-Sepulveda, Maria Del Rosario
AU  - Torres-Sepulveda Mdel R
AD  - Departamento de Genetica, Universidad Autonoma de Nuevo Leon, Hospital 
      Universitario, "Dr. Jose Eleuterio Gonzalez", Av. Gonzalitos s/n, Colonia Mitras 
      Centro, Monterrey, Nuevo Leon 64460 Mexico.
FAU - Ruiz-Herrera, Consuelo
AU  - Ruiz-Herrera C
AD  - Departamento de Genetica, Universidad Autonoma de Nuevo Leon, Hospital 
      Universitario, "Dr. Jose Eleuterio Gonzalez", Av. Gonzalitos s/n, Colonia Mitras 
      Centro, Monterrey, Nuevo Leon 64460 Mexico.
FAU - Cerda-Flores, Ricardo Martin
AU  - Cerda-Flores RM
AD  - Universidad Autonoma de Nuevo Leon, Facultad de Enfermeria, Avenida Gonzalitos, 
      1500 Norte, Col. Mitras Centro, Monterrey, NL Mexico.
FAU - Castillo-Garcia, Erik Ruben
AU  - Castillo-Garcia ER
AD  - Departamento de Genetica, Universidad Autonoma de Nuevo Leon, Hospital 
      Universitario, "Dr. Jose Eleuterio Gonzalez", Av. Gonzalitos s/n, Colonia Mitras 
      Centro, Monterrey, Nuevo Leon 64460 Mexico.
FAU - Rodriguez-Sanchez, Iram Pablo
AU  - Rodriguez-Sanchez IP
AD  - Departamento de Genetica, Universidad Autonoma de Nuevo Leon, Hospital 
      Universitario, "Dr. Jose Eleuterio Gonzalez", Av. Gonzalitos s/n, Colonia Mitras 
      Centro, Monterrey, Nuevo Leon 64460 Mexico.
FAU - Martinez de Villarreal, Laura Elia
AU  - Martinez de Villarreal LE
AD  - Departamento de Genetica, Universidad Autonoma de Nuevo Leon, Hospital 
      Universitario, "Dr. Jose Eleuterio Gonzalez", Av. Gonzalitos s/n, Colonia Mitras 
      Centro, Monterrey, Nuevo Leon 64460 Mexico.
LA  - eng
PT  - Journal Article
DEP - 20141127
PL  - England
TA  - Diabetol Metab Syndr
JT  - Diabetology & metabolic syndrome
JID - 101488958
PMC - PMC4416397
OTO - NOTNLM
OT  - Acetylcarnitine
OT  - Air plethysmography
OT  - Beta-oxidation defect
OT  - Butyrylcarnitine
OT  - Non-diabetic obese
OT  - T2DM
EDAT- 2014/01/01 00:00
MHDA- 2014/01/01 00:01
PMCR- 2014/11/27
CRDT- 2015/05/05 06:00
PHST- 2014/10/18 00:00 [received]
PHST- 2014/11/19 00:00 [accepted]
PHST- 2015/05/05 06:00 [entrez]
PHST- 2014/01/01 00:00 [pubmed]
PHST- 2014/01/01 00:01 [medline]
PHST- 2014/11/27 00:00 [pmc-release]
AID - 420 [pii]
AID - 10.1186/1758-5996-6-129 [doi]
PST - epublish
SO  - Diabetol Metab Syndr. 2014 Nov 27;6:129. doi: 10.1186/1758-5996-6-129. 
      eCollection 2014.