PMID- 25938036
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20150504
LR  - 20220330
IS  - 2222-3959 (Print)
IS  - 2227-4898 (Electronic)
IS  - 2222-3959 (Linking)
VI  - 8
IP  - 2
DP  - 2015
TI  - In vitro induction and differentiation of umbilical cord mesenchymal stem cells 
      into neuron-like cells by all-trans retinoic acid.
PG  - 250-6
LID - 10.3980/j.issn.2222-3959.2015.02.07 [doi]
AB  - AIM: To determine the optimal concentration for inducing the differentiation of 
      human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) into neuron-like 
      cells, although it is understood that all-trans retinoic acid (ATRA) regulates 
      cell proliferation in the nervous system by modulating the balance between 
      mitosis and apoptosis. METHODS: The abilities of ATRA to promote apoptosis as 
      well as neural differentiation were assessed in cultured hUC-MSCs by 
      morphological observation, MTT assay, annexin V-FITC/PI flow cytometry and 
      immunocytochemistry. RESULTS: The data showed that low concentrations of ATRA 
      (0.5 micromol, 0.25 micromol) had no effect on the number of cells. However, treatment 
      with 1.0 micromol or 2.0 micromol ATRA induced a 24.16% and 52.67% reduction in cell 
      number, respectively, compared with vehicle-treated cultures. Further, 4.0 micromol 
      ATRA had a potent effect on cell number, with almost no adherent cells recovered 
      after 24h. We further showed that 0.5 micromol ATRA caused these cells to express 
      characteristic markers of neuronal progenitor cells. CONCLUSION: Taken together, 
      we conclude that ATRA has a dose-dependent influence on the neural 
      differentiation and apoptosis of hUC-MSCs. These findings have implications on 
      the use of ATRA-differentiated hUC-MSCs for the study of neural degeneration 
      diseases.
FAU - Jin, Wei
AU  - Jin W
AD  - Eye Center, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, 
      China.
FAU - Xu, Yao-Peng
AU  - Xu YP
AD  - Department of Urology, Wuhan General Hospital of Guangzhou Military Command, 
      Wuhan 430072, Hubei Province, China.
FAU - Yang, An-Huai
AU  - Yang AH
AD  - Eye Center, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, 
      China.
FAU - Xing, Yi-Qiao
AU  - Xing YQ
AD  - Eye Center, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20150418
PL  - China
TA  - Int J Ophthalmol
JT  - International journal of ophthalmology
JID - 101553860
PMC - PMC4413565
OTO - NOTNLM
OT  - apoptosis
OT  - differentiation
OT  - human umbilical cord mesenchymal stem cells
OT  - neuron-like cells
EDAT- 2015/05/06 06:00
MHDA- 2015/05/06 06:01
PMCR- 2015/04/18
CRDT- 2015/05/05 06:00
PHST- 2014/07/16 00:00 [received]
PHST- 2014/11/06 00:00 [accepted]
PHST- 2015/05/05 06:00 [entrez]
PHST- 2015/05/06 06:00 [pubmed]
PHST- 2015/05/06 06:01 [medline]
PHST- 2015/04/18 00:00 [pmc-release]
AID - ijo-08-02-250 [pii]
AID - 10.3980/j.issn.2222-3959.2015.02.07 [doi]
PST - epublish
SO  - Int J Ophthalmol. 2015 Apr 18;8(2):250-6. doi: 
      10.3980/j.issn.2222-3959.2015.02.07. eCollection 2015.