PMID- 25942429
OWN - NLM
STAT- MEDLINE
DCOM- 20170131
LR  - 20171116
IS  - 1804-7521 (Electronic)
IS  - 1213-8118 (Linking)
VI  - 160
IP  - 1
DP  - 2016 Mar
TI  - TLR2 and TLR4 expression on CD14(++) and CD14(+) monocyte subtypes in adult-onset 
      autoimmune diabetes.
PG  - 76-83
LID - 10.5507/bp.2015.016 [doi]
AB  - BACKGROUND: Peripheral blood monocytes are key effectors of innate immunity. 
      Dysfunction, changes in their counts or altered expression of cytokines and 
      pattern-recognition receptors on monocytes may contribute to the development of 
      the autoimmune type of diabetes mellitus (AD). AIMS: We aimed to analyze the 
      counts and proportions of the two main subtypes of monocyte cells, CD14(++) and 
      CD14(+), and to look for potential changes in the expression of toll-like 
      receptors 2 (TLR2) and 4 (TLR4) as well as cytokine prolactin (PRL) in 
      adult-onset AD, including diabetes mellitus type 1 (T1DM) and latent autoimmune 
      diabetes in adults (LADA). METHODS: We examined 21 T1DM patients, 9 patients with 
      LADA, 16 control patients with type 2 diabetes mellitus (T2DM) and 24 healthy 
      individuals. All diabetic patients were diagnosed after the age of 18 years. 
      Expression at the mRNA level was determined by quantitative PCR. Flow cytometry 
      was used to ascertain membrane expression and cell counts. RESULTS: T1DM patients 
      had fewer CD14(++) (P < 0.01) and CD14(+) (P < 0.0001) monocytes whereas T2DM 
      subjects showed decreased counts of CD14(+) monocytes compared to healthy 
      controls (P < 0.001). TLR2 protein expression was significantly increased in T1DM 
      CD14(+) monocytes compared to healthy controls (P < 0.05), while TLR4 expression 
      in T1DM CD14(++) cells was significantly lower (P < 0.0001). There was no 
      significant difference between the groups in terms of PRL mRNA expression in 
      monocytes. CONCLUSIONS: The observed changes in the proportions of both immune 
      cell types and in the expression of functional pattern-recognition receptors on 
      monocytes in the subjects examined may arise as a consequence of chronic 
      inflammation that accompanies long-term diabetes.
FAU - Cejkova, Pavlina
AU  - Cejkova P
AD  - Department of Anthropology and Human Genetics, Faculty of Science, Charles 
      University in Prague, Czech Republic.
AD  - Department of General Biology and Genetics, Third Faculty of Medicine, Charles 
      University in Prague, Czech Republic.
FAU - Nemeckova, Iva
AU  - Nemeckova I
AD  - Department of Anthropology and Human Genetics, Faculty of Science, Charles 
      University in Prague, Czech Republic.
FAU - Broz, Jan
AU  - Broz J
AD  - 2nd Department of Internal Medicine, Diabetes Center, Third Faculty of Medicine, 
      Charles University in Prague, Czech Republic.
FAU - Cerna, Marie
AU  - Cerna M
AD  - Department of General Biology and Genetics, Third Faculty of Medicine, Charles 
      University in Prague, Czech Republic.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150504
PL  - Czech Republic
TA  - Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub
JT  - Biomedical papers of the Medical Faculty of the University Palacky, Olomouc, 
      Czechoslovakia
JID - 101140142
RN  - 0 (Lipopolysaccharide Receptors)
RN  - 0 (RNA, Messenger)
RN  - 0 (Toll-Like Receptor 2)
RN  - 0 (Toll-Like Receptor 4)
SB  - IM
MH  - Adult
MH  - Case-Control Studies
MH  - Diabetes Mellitus, Type 1/*immunology
MH  - Diabetes Mellitus, Type 2/*immunology
MH  - Down-Regulation/physiology
MH  - Female
MH  - Flow Cytometry
MH  - Humans
MH  - Immunity, Innate/physiology
MH  - Leukocytes, Mononuclear/*metabolism
MH  - Lipopolysaccharide Receptors/metabolism
MH  - Male
MH  - Middle Aged
MH  - RNA, Messenger/metabolism
MH  - Real-Time Polymerase Chain Reaction
MH  - Toll-Like Receptor 2/*metabolism
MH  - Toll-Like Receptor 4/*metabolism
OTO - NOTNLM
OT  - CD14 monocyte
OT  - adult-onset autoimmune diabetes
OT  - inflammation
OT  - prolactin
OT  - toll-like receptor
EDAT- 2015/05/06 06:00
MHDA- 2017/02/01 06:00
CRDT- 2015/05/06 06:00
PHST- 2014/12/22 00:00 [received]
PHST- 2015/04/01 00:00 [accepted]
PHST- 2015/05/06 06:00 [entrez]
PHST- 2015/05/06 06:00 [pubmed]
PHST- 2017/02/01 06:00 [medline]
AID - 10.5507/bp.2015.016 [doi]
PST - ppublish
SO  - Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2016 Mar;160(1):76-83. doi: 
      10.5507/bp.2015.016. Epub 2015 May 4.