PMID- 25944706 OWN - NLM STAT- MEDLINE DCOM- 20160426 LR - 20150807 IS - 1879-0984 (Electronic) IS - 0166-0934 (Linking) VI - 222 DP - 2015 Sep 15 TI - Characterization and utility of phages bearing peptides with affinity to porcine reproductive and respiratory syndrome virus nsp7 protein. PG - 231-41 LID - S0166-0934(15)00170-6 [pii] LID - 10.1016/j.jviromet.2015.04.026 [doi] AB - High-affinity peptides to porcine reproductive and respiratory syndrome virus (PRRSV) nonstructural protein (nsp) 7 were identified using phage-display technology. Five 12-amino-acid peptide sequences were identified after six rounds of biopanning. A putative CD##WC motif was found in two different consensus peptides borne by phages 4 and 5. The peptides borne by phages 4, 5, and 6 were synthesized for subsequent experiments, according to the results of the binding assays. Immunofluorescence assay revealed that all these peptides recognized nsp7 in PRRSV-infected cells. Furthermore, the peptides demonstrated antiviral activities, with peptides 5 and 6 showing effective inhibition. Early peptide stimulation was associated with strong antiviral activity, and the inhibitory effects of the peptides were dose-dependent at 36 and 48 h post-infection. Peptide 5 was selected to detect the intracellular localization of nsp7 by confocal microscopy. This peptide had a similar effect to anti-nsp7 monoclonal antibody on nsp7. These results suggest that high-affinity peptides to PRRSV nsp7 could mimic the potential of nsp7 antibody as a diagnostic reagent for virus detection. Moreover, the peptides selected in this study represented a potentially effective antiviral candidate to inhibit PRRSV. CI - Copyright (c) 2015. Published by Elsevier B.V. FAU - Wang, Heng AU - Wang H AD - College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province 510642, People's Republic of China; Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou, Guangdong Province 510642, People's Republic of China; MOA Key Laboratory of Animal Vaccine Development, Guangzhou, Guangdong Province 510642, People's Republic of China. FAU - Liu, Rongchang AU - Liu R AD - College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province 510642, People's Republic of China; Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou, Guangdong Province 510642, People's Republic of China; MOA Key Laboratory of Animal Vaccine Development, Guangzhou, Guangdong Province 510642, People's Republic of China. FAU - Cui, Jin AU - Cui J AD - College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province 510642, People's Republic of China; Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou, Guangdong Province 510642, People's Republic of China; MOA Key Laboratory of Animal Vaccine Development, Guangzhou, Guangdong Province 510642, People's Republic of China. FAU - Deng, Shengchao AU - Deng S AD - College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province 510642, People's Republic of China; Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou, Guangdong Province 510642, People's Republic of China; MOA Key Laboratory of Animal Vaccine Development, Guangzhou, Guangdong Province 510642, People's Republic of China. FAU - Xie, Jiexiong AU - Xie J AD - College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province 510642, People's Republic of China; Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou, Guangdong Province 510642, People's Republic of China; MOA Key Laboratory of Animal Vaccine Development, Guangzhou, Guangdong Province 510642, People's Republic of China. FAU - Nin, Zhangyong AU - Nin Z AD - College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province 510642, People's Republic of China; Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou, Guangdong Province 510642, People's Republic of China; MOA Key Laboratory of Animal Vaccine Development, Guangzhou, Guangdong Province 510642, People's Republic of China. FAU - Zhang, Guihong AU - Zhang G AD - College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province 510642, People's Republic of China; Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou, Guangdong Province 510642, People's Republic of China; MOA Key Laboratory of Animal Vaccine Development, Guangzhou, Guangdong Province 510642, People's Republic of China. Electronic address: guihongzh@scau.edu.cn. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20150502 PL - Netherlands TA - J Virol Methods JT - Journal of virological methods JID - 8005839 RN - 0 (Antiviral Agents) RN - 0 (Indicators and Reagents) RN - 0 (Peptide Library) RN - 0 (Peptides) RN - 0 (Viral Nonstructural Proteins) SB - IM MH - Animals MH - Antiviral Agents/isolation & purification/*metabolism MH - Diagnostic Tests, Routine/methods MH - Drug Therapy/methods MH - Indicators and Reagents/isolation & purification/*metabolism MH - Mass Screening MH - *Peptide Library MH - Peptides/isolation & purification/*metabolism MH - Porcine Reproductive and Respiratory Syndrome/diagnosis/drug therapy MH - Porcine respiratory and reproductive syndrome virus/*drug effects MH - Protein Binding MH - Swine MH - Viral Nonstructural Proteins/*metabolism OTO - NOTNLM OT - Antiviral activity OT - PRRSV OT - Peptide OT - Phage display OT - nsp7 protein EDAT- 2015/05/07 06:00 MHDA- 2016/04/27 06:00 CRDT- 2015/05/07 06:00 PHST- 2015/02/07 00:00 [received] PHST- 2015/04/02 00:00 [revised] PHST- 2015/04/23 00:00 [accepted] PHST- 2015/05/07 06:00 [entrez] PHST- 2015/05/07 06:00 [pubmed] PHST- 2016/04/27 06:00 [medline] AID - S0166-0934(15)00170-6 [pii] AID - 10.1016/j.jviromet.2015.04.026 [doi] PST - ppublish SO - J Virol Methods. 2015 Sep 15;222:231-41. doi: 10.1016/j.jviromet.2015.04.026. Epub 2015 May 2.