PMID- 25945236
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20150506
LR  - 20220330
IS  - 2008-3866 (Print)
IS  - 2008-3874 (Electronic)
IS  - 2008-3866 (Linking)
VI  - 18
IP  - 3
DP  - 2015 Mar
TI  - Acute and chronic effects of lithium on BDNF and GDNF mRNA and protein levels in 
      rat primary neuronal, astroglial and neuroastroglia cultures.
PG  - 240-6
AB  - OBJECTIVES: The neuroprotective effect of lithium has been attributed to its 
      therapeutic action. However, the role of glial cells particularly astrocytes, and 
      the possible interactions between neurons and astrocytes in neuroprotective 
      effects of lithium have been disregarded. Thus, the aim of this study was to 
      evaluate the direct effects of lithium on brain derived neurotrophic factor 
      (BDNF) and glial cell line derived neurotrophic factor (GDNF) in rat primary 
      neuronal, astrocytes, and mixed neuro-astroglial cultures to assess the possible 
      effects of lithium on astrocytes and neuro-astroglia interactions. MATERIALS AND 
      METHODS: Rat primary astrocyte, neuronal and mixed neuro-astrocyte cultures were 
      prepared from cortices of 18-day embryos. Cell cultures were exposed to lithium 
      (1 mM) or vehicle for 1 day (acute) or 7 days (chronic). BDNF and GDNF mRNA and 
      protein levels were determined by RT-PCR and ELISA, respectively. RESULTS: 
      Chronic but not acute lithium treatment increased intracellular BDNF and GDNF 
      protein levels in rat primary neuronal and astrocyte cultures, respectively 
      (P<0.05). However, chronic lithium treatment had no significant effect on 
      intracellular BDNF protein level in astrocyte and mixed neuron-astrocyte cultures 
      or GDNF protein levels in mixed neuron-astrocyte culture. Furthermore, acute and 
      chronic lithium treatment had no significant effect on mRNA and extracellular 
      BDNF and GDNF protein levels in three studied cultures. CONCLUSION: Present study 
      showed that chronic lithium treatment affected neurotrophins both in neurons and 
      astrocytes in a cell-type specific manner with no effect on neuron-astrocyte 
      interactions. The findings of this study also highlighted the importance of 
      astrocytes as drug targets involved in the neuroprotective action of lithium.
FAU - Emamghoreishi, Masoumeh
AU  - Emamghoreishi M
AD  - Department of Pharmacology, School of Medicine, Shiraz University of Medical 
      Sciences, Shiraz, Iran ; Department of Neuroscience, School of Advanced Medical 
      Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran.
FAU - Keshavarz, Mojtaba
AU  - Keshavarz M
AD  - Department of Pharmacology, School of Medicine, Shiraz University of Medical 
      Sciences, Shiraz, Iran.
FAU - Nekooeian, Ali Akbar
AU  - Nekooeian AA
AD  - Department of Pharmacology, School of Medicine, Shiraz University of Medical 
      Sciences, Shiraz, Iran.
LA  - eng
PT  - Journal Article
PL  - Iran
TA  - Iran J Basic Med Sci
JT  - Iranian journal of basic medical sciences
JID - 101517966
PMC - PMC4414989
OTO - NOTNLM
OT  - Astrocytes
OT  - BDNF
OT  - Culture
OT  - GDNF
OT  - Lithium
OT  - Neuro-astroglial
OT  - Neurons
EDAT- 2015/05/07 06:00
MHDA- 2015/05/07 06:01
PMCR- 2015/03/01
CRDT- 2015/05/07 06:00
PHST- 2014/06/30 00:00 [received]
PHST- 2014/10/19 00:00 [accepted]
PHST- 2015/05/07 06:00 [entrez]
PHST- 2015/05/07 06:00 [pubmed]
PHST- 2015/05/07 06:01 [medline]
PHST- 2015/03/01 00:00 [pmc-release]
AID - IJBMS-18-240 [pii]
PST - ppublish
SO  - Iran J Basic Med Sci. 2015 Mar;18(3):240-6.