PMID- 25945332
OWN - NLM
STAT- MEDLINE
DCOM- 20160201
LR  - 20181113
IS  - 2314-6141 (Electronic)
IS  - 2314-6133 (Print)
VI  - 2015
DP  - 2015
TI  - Regulation of Osteoblast Differentiation by Acid-Etched and/or Grit-Blasted 
      Titanium Substrate Topography Is Enhanced by 1,25(OH)2D3 in a Sex-Dependent 
      Manner.
PG  - 365014
LID - 10.1155/2015/365014 [doi]
LID - 365014
AB  - This study assessed contributions of micron-scale topography on clinically 
      relevant titanium (Ti) to differentiation of osteoprogenitor cells and 
      osteoblasts; the interaction of this effect with 1alpha,25-dihydroxyvitamin D3 
      (1alpha,25(OH)2D3); and if the effects are sex-dependent. Male and female rat bone 
      marrow cells (BMCs) were cultured on acid-etched (A, R a = 0.87 mum), grit-blasted 
      (GB, R a = 3.90 mum), or grit-blasted/acid-etched (SLA, R a = 3.22 mum) Ti. BMCs 
      were sensitive to surface topography and underwent osteoblast differentiation. 
      This was greatest on SLA; acid etching and grit blasting contributed additively. 
      Primary osteoblasts were also sensitive to SLA, with less effect from individual 
      structural components, demonstrated by enhanced local factor production. 
      Sex-dependent responses of BMCs to topography varied with parameter whereas male 
      and female osteoblasts responded similarly to surface treatment. 1alpha,25(OH)2D3 
      enhanced cell responses on all surfaces similarly. Effects were sex-dependent and 
      male cells grown on a complex microstructured surface were much more sensitive 
      than female cells. These results indicate that effects of the complex SLA 
      topography are greater than acid etching or grit blasting alone on multipotent 
      BMCs and committed osteoblasts and that individual parameters are sex-specific. 
      The effect of 1alpha,25(OH)2D3 was sex dependent. The results also suggest that 
      levels of 1alpha,25(OH)2D3 in the patient may be important in osseointegration.
FAU - Olivares-Navarrete, Rene
AU  - Olivares-Navarrete R
AD  - Department of Biomedical Engineering, School of Engineering, Virginia 
      Commonwealth University, 601 W. Main Street, Richmond, VA 23284, USA.
FAU - Hyzy, Sharon L
AU  - Hyzy SL
AD  - Department of Biomedical Engineering, School of Engineering, Virginia 
      Commonwealth University, 601 W. Main Street, Richmond, VA 23284, USA.
FAU - Boyan, Barbara D
AU  - Boyan BD
AD  - Department of Biomedical Engineering, School of Engineering, Virginia 
      Commonwealth University, 601 W. Main Street, Richmond, VA 23284, USA ; Department 
      of Biomedical Engineering, Georgia Institute of Technology, 313 Ferst Drive NW, 
      Atlanta, GA 30332, USA.
FAU - Schwartz, Zvi
AU  - Schwartz Z
AD  - Department of Biomedical Engineering, School of Engineering, Virginia 
      Commonwealth University, 601 W. Main Street, Richmond, VA 23284, USA ; Department 
      of Periodontics, University of Texas Health Science Center at San Antonio, 7703 
      Floyd Curl Drive, San Antonio, TX 78229, USA.
LA  - eng
GR  - R01 AR052102/AR/NIAMS NIH HHS/United States
GR  - AR052102/AR/NIAMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20150406
PL  - United States
TA  - Biomed Res Int
JT  - BioMed research international
JID - 101600173
RN  - 0 (dihydroxy-vitamin D3)
RN  - 1406-16-2 (Vitamin D)
RN  - D1JT611TNE (Titanium)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/cytology/drug effects
MH  - Cell Differentiation/*drug effects
MH  - Female
MH  - Humans
MH  - Male
MH  - Osseointegration/drug effects
MH  - Osteoblasts/cytology/drug effects
MH  - Osteogenesis/*drug effects
MH  - Rats
MH  - Sex Characteristics
MH  - Titanium/*chemistry
MH  - Vitamin D/analogs & derivatives/pharmacology
PMC - PMC4402479
EDAT- 2015/05/07 06:00
MHDA- 2016/02/02 06:00
PMCR- 2015/04/06
CRDT- 2015/05/07 06:00
PHST- 2014/07/25 00:00 [received]
PHST- 2014/09/14 00:00 [accepted]
PHST- 2015/05/07 06:00 [entrez]
PHST- 2015/05/07 06:00 [pubmed]
PHST- 2016/02/02 06:00 [medline]
PHST- 2015/04/06 00:00 [pmc-release]
AID - 10.1155/2015/365014 [doi]
PST - ppublish
SO  - Biomed Res Int. 2015;2015:365014. doi: 10.1155/2015/365014. Epub 2015 Apr 6.