PMID- 25947661
OWN - NLM
STAT- MEDLINE
DCOM- 20160202
LR  - 20150507
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1313
DP  - 2015
TI  - FISH Detection of X and Y Chromosomes in Combination with Immunofluorescence to 
      Study Contribution of Transplanted Cells to Skeletal Muscle Fibers.
PG  - 131-9
LID - 10.1007/978-1-4939-2703-6_9 [doi]
AB  - During the past decades, several studies in animals have displayed the ability of 
      cells from the bone marrow (BM) to participate in regeneration of various tissues 
      including skeletal muscle tissue. Studies in mice have demonstrated that regular 
      physical activity is sufficient to induce contribution of BM derived cells to the 
      skeletal muscle tissue, suggesting that this is part of the physiological 
      remodeling of skeletal muscle. To analyze whether BM-derived cells participate in 
      skeletal muscle remodeling in human, we developed a protocol of 
      immunofluorescence in combination with fluorescence in situ hybridization (FISH) 
      that enables the detection of male donor bone marrow cell contribution to female 
      skeletal muscle tissue.
FAU - Stromberg, Anna
AU  - Stromberg A
AD  - Division of Clinical Physiology, Department of Laboratory Medicine, Karolinska 
      Institutet, Karolinska University Hospital Huddinge, 141 86, Stockholm, Sweden, 
      anna.stromberg@ki.se.
FAU - Jansson, Monika
AU  - Jansson M
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/cytology/*metabolism
MH  - Cell Differentiation
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Male
MH  - Mice
MH  - Muscle Fibers, Skeletal/cytology/*metabolism
MH  - *X Chromosome
MH  - *Y Chromosome
EDAT- 2015/05/08 06:00
MHDA- 2016/02/03 06:00
CRDT- 2015/05/08 06:00
PHST- 2015/05/08 06:00 [entrez]
PHST- 2015/05/08 06:00 [pubmed]
PHST- 2016/02/03 06:00 [medline]
AID - 10.1007/978-1-4939-2703-6_9 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2015;1313:131-9. doi: 10.1007/978-1-4939-2703-6_9.