PMID- 2595079
OWN - NLM
STAT- MEDLINE
DCOM- 19900124
LR  - 20191022
IS  - 0923-2494 (Print)
IS  - 0923-2494 (Linking)
VI  - 140
IP  - 7
DP  - 1989 Sep
TI  - Natural anti-TNP antibodies from rainbow trout interfere with viral infection in 
      vitro.
PG  - 675-84
AB  - Normal and viral-infected rainbow trout (RT) were tested for serum antibody 
      activity against self and nonself antigens. Particularly high titres of 
      anti-trinitrophenyl (TNP) antibodies were noted, as in other fish species. To 
      analyse this, the anti-TNP antibodies were isolated by affinity chromatography 
      and their capacity to interfere with viral infection in vitro was studied. We 
      selected RT fibroblasts as target cells, and two common pathogenic viruses in 
      trout, a rhabdovirus, viral haemorrhagic septicaemia virus (VHS) and a 
      birnavirus, the infectious pancreatic necrosis virus (IPN). Anti-TNP antibodies 
      were examined for their capacity to neutralize VHS and IPN viruses. Data obtained 
      show that the anti-TNP antibodies, even at high concentrations, only partially 
      neutralized virus. In contrast, when anti-TNP antibodies were assayed for their 
      protective activity using RT fibroblast cells infected with VHS or IPN viruses, 
      results showed high protective activity, regardless of serum origin or of the 
      virus used, when the antibodies were added to the cell culture after viral 
      infection. Therefore, our experiments indicate that the protective activity does 
      not seem to be due to a direct interaction of the antibodies with the viruses. It 
      is suggested that virus-modified cell surface self structures exhibit new 
      epitopes which interact with the anti-TNP antibodies. Such an interaction would 
      allow anti-TNP antibodies to participate in a non-specific defence mechanism 
      against viral infection.
FAU - Gonzalez, R
AU  - Gonzalez R
AD  - Unite d'Immunocytochimie, CNRS URA 359, Institut Pasteur.
FAU - Matsiota, P
AU  - Matsiota P
FAU - Torchy, C
AU  - Torchy C
FAU - De Kinkelin, P
AU  - De Kinkelin P
FAU - Avrameas, S
AU  - Avrameas S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - France
TA  - Res Immunol
JT  - Research in immunology
JID - 8907467
RN  - 0 (Antibodies)
RN  - 0 (Nitrobenzenes)
RN  - 0 (Trinitrobenzenes)
SB  - IM
MH  - Animals
MH  - Antibodies/*immunology
MH  - Immunity, Innate
MH  - Nitrobenzenes/*immunology
MH  - Protein Conformation
MH  - Trinitrobenzenes/*immunology
MH  - Trout
MH  - Virus Diseases/*immunology
EDAT- 1989/09/01 00:00
MHDA- 1989/09/01 00:01
CRDT- 1989/09/01 00:00
PHST- 1989/09/01 00:00 [pubmed]
PHST- 1989/09/01 00:01 [medline]
PHST- 1989/09/01 00:00 [entrez]
AID - 0923-2494(89)90021-7 [pii]
AID - 10.1016/0923-2494(89)90021-7 [doi]
PST - ppublish
SO  - Res Immunol. 1989 Sep;140(7):675-84. doi: 10.1016/0923-2494(89)90021-7.