PMID- 25951665 OWN - NLM STAT- MEDLINE DCOM- 20150602 LR - 20221207 IS - 0031-7144 (Print) IS - 0031-7144 (Linking) VI - 69 IP - 12 DP - 2014 Dec TI - Venlafaxine inhibits apoptosis of hippocampal neurons by up-regulating brain-derived neurotrophic factor in a rat depression model. PG - 909-16 AB - The effect of venlafaxine on the expression of brain-derived neurotrophic factor (BDNF) in rat hippocampal neurons was studied, as well as its inhibitory effect on apoptosis of hippocampal neurons. Differences in behavioral ability between the depression model group and the venlafaxine treatment group were observed in behavioral, sucrose-water and open field tests. The rat hippocampal tissue was sliced, stained and observed for BDNF distribution by immunohistochemistry. Apoptosis of hippocampal neurons was detected by TUNEL. BDNF expression in the hippocampal tissue was detected by Western blot. Injury and apoptosis of the hippocampal tissue were observed by electron microscopy. Behavioral test showed that venlafaxine effectively improved the behavioral abilities of depressed rats. Immunohistochemistry showed that venlafaxine markedly increased BDNF expression in the rat hippocampus. TUNEL showed that venlafaxine markedly inhibited apoptosis of hippocampal neurons, which was also confirmed by electron microscopic observation of the pathologic sections. Venlafaxine improved the expression of BDNF by influencing the PI3k/PKB/eNOS pathway and repressed the apoptosis of hippocampal neurons. FAU - Huang, Xiao AU - Huang X FAU - Mao, Yue-Shi AU - Mao YS FAU - Li, Chao AU - Li C FAU - Wang, Hao AU - Wang H FAU - Ji, Jian-Lin AU - Ji JL LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - Germany TA - Pharmazie JT - Die Pharmazie JID - 9800766 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Cyclohexanols) RN - 0 (Serotonin Uptake Inhibitors) RN - 31C4KY9ESH (Nitric Oxide) RN - 7D7RX5A8MO (Venlafaxine Hydrochloride) SB - IM MH - Animals MH - Apoptosis/*drug effects MH - Behavior, Animal/drug effects MH - Brain-Derived Neurotrophic Factor/*biosynthesis MH - Cyclohexanols/*pharmacology MH - Depression/*metabolism/*pathology MH - Hippocampus/*drug effects/*pathology MH - Male MH - Neurons/drug effects/*metabolism/*pathology MH - Nitric Oxide/metabolism MH - Pyramidal Cells/drug effects MH - Rats MH - Rats, Sprague-Dawley MH - Selective Serotonin Reuptake Inhibitors/*pharmacology MH - Signal Transduction/drug effects MH - Up-Regulation/drug effects MH - Venlafaxine Hydrochloride MH - Weight Gain/drug effects EDAT- 2015/05/09 06:00 MHDA- 2015/06/03 06:00 CRDT- 2015/05/09 06:00 PHST- 2015/05/09 06:00 [entrez] PHST- 2015/05/09 06:00 [pubmed] PHST- 2015/06/03 06:00 [medline] PST - ppublish SO - Pharmazie. 2014 Dec;69(12):909-16.