PMID- 25951823
OWN - NLM
STAT- MEDLINE
DCOM- 20160210
LR  - 20181113
IS  - 1471-2490 (Electronic)
IS  - 1471-2490 (Linking)
VI  - 15
DP  - 2015 May 8
TI  - Expression of brain derived-neurotrophic factor and granulocyte-colony 
      stimulating factor in the urothelium: relation with voiding function.
PG  - 37
LID - 10.1186/s12894-015-0036-3 [doi]
LID - 37
AB  - BACKGROUND: We designed this experiment to elucidate the relationship between the 
      expression of brain derived-neurotrophic factor (BDNF), the expression of 
      granulocyte-colony stimulating factor (G-CSF), and the development of overactive 
      bladder (OAB). In our previous study, the urothelium was observed to be more than 
      a simple mechanosensory receptor and was found to be a potential therapeutic 
      target for OAB. Moreover, neuregulin-1 and BDNF were found to be potential new 
      biomarkers of OAB. Here, we investigated the relationship between changes in the 
      voiding pattern and the expression of BDNF and G-CSF in the urothelium and 
      evaluated the effects of 5-hydroxymethyl tolterodine (5-HMT) on rats with bladder 
      outlet obstruction (BOO). METHODS: A total of 100 Sprague-Dawley rats were 
      divided into the following groups: 20 control rats; 40 BOO rats; and 40 BOO rats 
      administered 5-HMT (0.1 mg/kg). After BOO was induced for 4 weeks, the rats were 
      assessed by cystometrography. The changes in BDNF and G-CSF expression were 
      examined in both separated urothelial tissues and in cultured urothelial cells by 
      reverse transcription polymerase chain reaction (RT-PCR). RESULTS: BOO rats 
      showed increased non-voiding activity [NVA; (number/10 voidings)] and bladder 
      weight and decreased micturition volume (MV), micturition interval (MI), and 
      micturition time (MT) relative to the controls. Moreover, the 5-HMT 
      administration rats showed decreased NVA and bladder weight and increased MV and 
      MI in comparison to the BOO rats. BDNF and G-CSF expression was increased in BOO 
      rats and decreased following 5-HMT administration. In this model, voiding 
      dysfunction developed as a result of BOO. As a therapeutic agent for OAB, the 
      administration of 5-HMT improved the voiding dysfunction. CONCLUSIONS: BDNF and 
      G-CSF might modulate voiding patterns through micturition pathways and might be 
      involved only in the urothelium. Moreover, the expression of both genes in the 
      urothelium might be related to voiding dysfunction in OAB patients. Thus, the 
      urothelium has an important role in the manifestation of voiding symptoms.
FAU - Yuk, Seung Mo
AU  - Yuk SM
AD  - Department of Urology, Korea St. Mary's Hospital, College of Medicine, The 
      Catholic University of Korea, Seoul, South Korea. assa7913@naver.com.
FAU - Shin, Ju Hyun
AU  - Shin JH
AD  - Department of Urology, Korea Chungnam National University Hospital, College of 
      Medicine, Chungnam National University, Daejeon, South Korea. 
      synthia2000@naver.com.
FAU - Song, Ki Hak
AU  - Song KH
AD  - Department of Urology, Korea Chungnam National University Hospital, College of 
      Medicine, Chungnam National University, Daejeon, South Korea. urosong@cnu.ac.kr.
FAU - Na, Yong Gil
AU  - Na YG
AD  - Department of Urology, Korea Chungnam National University Hospital, College of 
      Medicine, Chungnam National University, Daejeon, South Korea. yongna@cnu.ac.kr.
FAU - Lim, Jae Sung
AU  - Lim JS
AD  - Department of Urology, Korea Chungnam National University Hospital, College of 
      Medicine, Chungnam National University, Daejeon, South Korea. uro17@cnu.ac.kr.
FAU - Sul, Chong Koo
AU  - Sul CK
AD  - Department of Urology, Korea Chungnam National University Hospital, College of 
      Medicine, Chungnam National University, Daejeon, South Korea. doctor6@daum.net.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150508
PL  - England
TA  - BMC Urol
JT  - BMC urology
JID - 100968571
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 143011-72-7 (Granulocyte Colony-Stimulating Factor)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Disease Models, Animal
MH  - Granulocyte Colony-Stimulating Factor/*metabolism
MH  - Rats, Sprague-Dawley
MH  - Urinary Bladder, Overactive/metabolism/*physiopathology
MH  - Urination/*physiology
MH  - Urothelium/*metabolism
PMC - PMC4436171
EDAT- 2015/05/09 06:00
MHDA- 2016/02/11 06:00
PMCR- 2015/05/08
CRDT- 2015/05/09 06:00
PHST- 2015/01/06 00:00 [received]
PHST- 2015/04/30 00:00 [accepted]
PHST- 2015/05/09 06:00 [entrez]
PHST- 2015/05/09 06:00 [pubmed]
PHST- 2016/02/11 06:00 [medline]
PHST- 2015/05/08 00:00 [pmc-release]
AID - 10.1186/s12894-015-0036-3 [pii]
AID - 36 [pii]
AID - 10.1186/s12894-015-0036-3 [doi]
PST - epublish
SO  - BMC Urol. 2015 May 8;15:37. doi: 10.1186/s12894-015-0036-3.