PMID- 25953328
OWN - NLM
STAT- Publisher
LR  - 20240227
IS  - 1097-4652 (Electronic)
IS  - 0021-9541 (Linking)
DP  - 2015 May 7
TI  - Protein Kinase C Is Involved in the Induction of ATP-Binding Cassette Transporter 
      A1 Expression by Liver X Receptor/Retinoid X Receptor Agonist in Human 
      Macrophages.
LID - 10.1002/jcp.25157 [doi]
AB  - The transcription of the ATP-binding cassette transporter A1 (ABCA1) gene, which 
      plays a key anti-atherogenic role, is known to be induced by agonists of liver X 
      receptors (LXRs). LXRs form obligate heterodimers with retinoid X receptors 
      (RXRs) and interact with their recognition sequences in the regulatory regions of 
      key genes implicated in the control of cholesterol, fatty acid and glucose 
      homeostasis. We have previously shown a novel role for c-Jun N-terminal kinase 
      (JNK) and phosphoinositide 3-kinase (PI3K) in the LXRs-mediated induction of 
      macrophage gene expression. Protein kinase C (PKC) is often found to regulate the 
      action of nuclear receptors and cross talk between this kinase family and JNK 
      and/or PI3K has been shown in several settings. We have therefore investigated a 
      potential role for PKC in the action of LXR/RXR agonist 22-(R)-hydroxycholesterol 
      (22-(R)-HC)/9-cis-retinoic acid (9cRA) in THP-1 macrophages, including the 
      induction of ABCA1 expression. The pan PKC inhibitor bisindoylmaleimide was found 
      to attenuate the induction of ABCA1 protein expression, the activation of the JNK 
      signaling pathway and the stimulation of activator protein-1 (AP-1) DNA binding 
      activity in macrophages treated with 22-(R)-HC and 9cRA. The role of PKC in the 
      action of these ligands was confirmed further by the use of more isotype-specific 
      inhibitors. These studies therefore reveal a potentially important role for PKC 
      in the action of 22-(R)-HC and 9cRA in human macrophages. This article is 
      protected by copyright. All rights reserved.
CI  - This article is protected by copyright. All rights reserved.
FAU - Huwait, Etimad A
AU  - Huwait EA
AD  - Cardiff School of Biosciences, Cardiff University, Sir Martin Evans Building, 
      Museum Avenue, Cardiff, CF10 3AX, United Kingdom.
FAU - Singh, Nishi N
AU  - Singh NN
AD  - Cardiff School of Biosciences, Cardiff University, Sir Martin Evans Building, 
      Museum Avenue, Cardiff, CF10 3AX, United Kingdom.
FAU - Michael, Daryn R
AU  - Michael DR
AD  - Cardiff School of Biosciences, Cardiff University, Sir Martin Evans Building, 
      Museum Avenue, Cardiff, CF10 3AX, United Kingdom.
FAU - Davies, Thomas S
AU  - Davies TS
AD  - Cardiff School of Biosciences, Cardiff University, Sir Martin Evans Building, 
      Museum Avenue, Cardiff, CF10 3AX, United Kingdom.
FAU - Moss, Joe W E
AU  - Moss JW
AD  - Cardiff School of Biosciences, Cardiff University, Sir Martin Evans Building, 
      Museum Avenue, Cardiff, CF10 3AX, United Kingdom.
FAU - Ramji, Dipak P
AU  - Ramji DP
AD  - Cardiff School of Biosciences, Cardiff University, Sir Martin Evans Building, 
      Museum Avenue, Cardiff, CF10 3AX, United Kingdom.
LA  - eng
PT  - Journal Article
PT  - Retracted Publication
DEP - 20150507
PL  - United States
TA  - J Cell Physiol
JT  - Journal of cellular physiology
JID - 0050222
RIN - J Cell Physiol. 2016 Apr;231(4):964. PMID: 27392327
OTO - NOTNLM
OT  - Atherosclerosis
OT  - Cell Signaling
OT  - Gene Expression
OT  - Liver X Receptors
OT  - Macrophages
OT  - Nuclear Receptors
EDAT- 2015/05/09 06:00
MHDA- 2015/05/09 06:00
CRDT- 2015/05/09 06:00
PHST- 2014/07/03 00:00 [received]
PHST- 2015/02/10 00:00 [revised]
PHST- 2015/03/03 00:00 [accepted]
PHST- 2015/05/09 06:00 [entrez]
PHST- 2015/05/09 06:00 [pubmed]
PHST- 2015/05/09 06:00 [medline]
AID - 10.1002/jcp.25157 [doi]
PST - aheadofprint
SO  - J Cell Physiol. 2015 May 7. doi: 10.1002/jcp.25157.