PMID- 25953398
OWN - NLM
STAT- MEDLINE
DCOM- 20150710
LR  - 20150508
IS  - 1879-3592 (Electronic)
IS  - 1383-5718 (Linking)
VI  - 783
DP  - 2015 May 1
TI  - The in vivo Pig-a assay: A report of the International Workshop On Genotoxicity 
      Testing (IWGT) Workgroup.
PG  - 23-35
LID - S1383-5718(14)00247-2 [pii]
LID - 10.1016/j.mrgentox.2014.09.007 [doi]
AB  - The in vivo Pig-a assay uses flow cytometry to measure phenotypic variants for 
      antibody binding to cell surface glycosylphosphatidylinositol (GPI)-anchored 
      proteins. There is good evidence suggesting that the absence of antibody binding 
      is the result of a mutation in the endogenous X-linked Pig-a gene, which forms 
      the rationale for the assay. Although the assay has been performed with several 
      types of hematopoietic cells and in a variety of mammalian species, including 
      humans, currently it is optimized only for measuring CD59-deficient (presumed 
      Pig-a mutant) erythrocytes in the peripheral blood of rats. An expert workgroup 
      formed by the International Workshop on Genotoxicity Testing considered the state 
      of assay development and the potential of the assay for regulatory use. Consensus 
      was reached on what is known about the Pig-a assay and how it should be 
      conducted, and recommendations were made on additional data and refinements that 
      would help to further enhance the assay for use in hazard identification and risk 
      assessment.
CI  - Published by Elsevier B.V.
FAU - Gollapudi, B Bhaskar
AU  - Gollapudi BB
AD  - E(x)ponent , Midland, MI, USA.
FAU - Lynch, Anthony M
AU  - Lynch AM
AD  - GlaxoSmithKline, Hertfordshire, UK.
FAU - Heflich, Robert H
AU  - Heflich RH
AD  - US Food and Drug Administration, National Center for Toxicological Research, 
      Jefferson, AR, USA. Electronic address: robert.heflich@fda.hhs.gov.
FAU - Dertinger, Stephen D
AU  - Dertinger SD
AD  - Litron Laboratories, Rochester, NY, USA.
FAU - Dobrovolsky, Vasily N
AU  - Dobrovolsky VN
AD  - US Food and Drug Administration, National Center for Toxicological Research, 
      Jefferson, AR, USA.
FAU - Froetschl, Roland
AU  - Froetschl R
AD  - Federal Institute for Drugs and Medical Devices, Bonn, Germany.
FAU - Horibata, Katsuyoshi
AU  - Horibata K
AD  - National Institute of Health Sciences, Tokyo, Japan.
FAU - Kenyon, Michelle O
AU  - Kenyon MO
AD  - Pfizer Global Research and Development, Groton, CT, USA.
FAU - Kimoto, Takafumi
AU  - Kimoto T
AD  - Teijin Pharma, Tokyo, Japan.
FAU - Lovell, David P
AU  - Lovell DP
AD  - St. George's, University of London, UK.
FAU - Stankowski, Leon F Jr
AU  - Stankowski LF Jr
AD  - BioReliance, Rockville, MD, USA.
FAU - White, Paul A
AU  - White PA
AD  - Environmental Health Science and Research Bureau, Health Canada, Ottawa, ON, 
      Canada.
FAU - Witt, Kristine L
AU  - Witt KL
AD  - National Institutes of Health, National Institute of Environmental Health 
      Sciences, Division of the National Toxicology Program, Research Triangle Park, 
      NC, USA.
FAU - Tanir, Jennifer Y
AU  - Tanir JY
AD  - Health and Environmental Sciences Institute, Washington, DC, USA.
LA  - eng
PT  - Journal Article
DEP - 20140926
PL  - Netherlands
TA  - Mutat Res Genet Toxicol Environ Mutagen
JT  - Mutation research. Genetic toxicology and environmental mutagenesis
JID - 101632149
RN  - 0 (Antibodies)
RN  - 0 (Membrane Proteins)
RN  - 0 (phosphatidylinositol glycan-class A protein)
RN  - CD59 Deficiency
SB  - IM
MH  - *Anemia, Hemolytic/metabolism/pathology
MH  - Animals
MH  - Antibodies/chemistry
MH  - Education
MH  - *Erythrocytes/metabolism/pathology
MH  - *Flow Cytometry/methods/standards
MH  - *Hemoglobinuria/metabolism/pathology
MH  - Humans
MH  - *Membrane Proteins/genetics/metabolism
MH  - *Mutation
MH  - Rats
OTO - NOTNLM
OT  - CD59
OT  - Flow cytometry
OT  - Glycosylphosphatidylinositol
OT  - Mutation
OT  - Red blood cells
OT  - Reticulocytes
EDAT- 2015/05/09 06:00
MHDA- 2015/07/15 06:00
CRDT- 2015/05/09 06:00
PHST- 2014/09/13 00:00 [received]
PHST- 2014/09/15 00:00 [accepted]
PHST- 2015/05/09 06:00 [entrez]
PHST- 2015/05/09 06:00 [pubmed]
PHST- 2015/07/15 06:00 [medline]
AID - S1383-5718(14)00247-2 [pii]
AID - 10.1016/j.mrgentox.2014.09.007 [doi]
PST - ppublish
SO  - Mutat Res Genet Toxicol Environ Mutagen. 2015 May 1;783:23-35. doi: 
      10.1016/j.mrgentox.2014.09.007. Epub 2014 Sep 26.