PMID- 25953566
OWN - NLM
STAT- MEDLINE
DCOM- 20160201
LR  - 20150508
IS  - 1873-0191 (Electronic)
IS  - 0928-4931 (Linking)
VI  - 52
DP  - 2015
TI  - Enhanced adherence of mouse fibroblast and vascular cells to plasma modified 
      polyethylene.
PG  - 259-66
LID - S0928-4931(15)00234-9 [pii]
LID - 10.1016/j.msec.2015.03.052 [doi]
AB  - Since the last decade, tissue engineering has shown a sensational promise in 
      providing more viable alternatives to surgical procedures for harvested tissues, 
      implants and prostheses. Biomedical polymers, such as low-density polyethylene 
      (LDPE), high-density polyethylene (HDPE) and ultra-high molecular weight 
      polyethylene (UHMWPE), were activated by Ar plasma discharge. Degradation of 
      polymer chains was examined by determination of the thickness of ablated layer. 
      The amount of an ablated polymer layer was measured by gravimetry. Contact angle, 
      measured by goniometry, was studied as a function of plasma exposure and 
      post-exposure aging times. Chemical structure of modified polymers was 
      characterized by angle resolved X-ray photoelectron spectroscopy. Surface 
      chemistry and polarity of the samples were investigated by electrokinetic 
      analysis. Changes in surface morphology were followed using atomic force 
      microscopy. Cytocompatibility of plasma activated polyethylene foils was studied 
      using two distinct model cell lines; VSMCs (vascular smooth muscle cells) as a 
      model for vascular graft testing and connective tissue cells L929 (mouse 
      fibroblasts) approved for standardized material cytotoxicity testing. 
      Specifically, the cell number, morphology, and metabolic activity of the adhered 
      and proliferated cells on the polyethylene matrices were studied in vitro. It was 
      found that the plasma treatment caused ablation of the polymers, resulting in 
      dramatic changes in their surface morphology and roughness. ARXPS and 
      electrokinetic measurements revealed oxidation of the polymer surface. It was 
      found that plasma activation has a positive effect on the adhesion and 
      proliferation of VSMCs and L929 cells.
CI  - Copyright (c) 2015 Elsevier B.V. All rights reserved.
FAU - Reznickova, Alena
AU  - Reznickova A
AD  - Department of Solid State Engineering, Institute of Chemical Technology Prague, 
      166 28 Prague 6, Czech Republic. Electronic address: alena.reznickova@vscht.cz.
FAU - Novotna, Zdenka
AU  - Novotna Z
AD  - Department of Solid State Engineering, Institute of Chemical Technology Prague, 
      166 28 Prague 6, Czech Republic. Electronic address: zdenka1.novotna@vscht.cz.
FAU - Kolska, Zdenka
AU  - Kolska Z
AD  - Faculty of Science, J.E. Purkyne University, 400 96 Usti nad Labem, Czech 
      Republic.
FAU - Kasalkova, Nikola Slepickova
AU  - Kasalkova NS
AD  - Department of Solid State Engineering, Institute of Chemical Technology Prague, 
      166 28 Prague 6, Czech Republic.
FAU - Rimpelova, Silvie
AU  - Rimpelova S
AD  - Department of Biochemistry and Microbiology, Institute of Chemical Technology 
      Prague, 166 28 Prague 6, Czech Republic.
FAU - Svorcik, Vaclav
AU  - Svorcik V
AD  - Department of Solid State Engineering, Institute of Chemical Technology Prague, 
      166 28 Prague 6, Czech Republic.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150325
PL  - Netherlands
TA  - Mater Sci Eng C Mater Biol Appl
JT  - Materials science & engineering. C, Materials for biological applications
JID - 101484109
RN  - 9002-88-4 (Polyethylene)
SB  - IM
MH  - Animals
MH  - Cell Adhesion/drug effects
MH  - Cell Line
MH  - Cell Proliferation/drug effects
MH  - Fibroblasts/cytology/drug effects
MH  - Mice
MH  - Muscle, Smooth, Vascular/cytology
MH  - Myocytes, Smooth Muscle/cytology/drug effects
MH  - Plasma/*chemistry
MH  - Polyethylene/*chemistry/*pharmacology
MH  - Rats
OTO - NOTNLM
OT  - Ablation
OT  - Aging
OT  - Cell adhesion
OT  - Metabolic activity
OT  - Plasma treatment
OT  - Polyethylene (PE)
EDAT- 2015/05/09 06:00
MHDA- 2016/02/02 06:00
CRDT- 2015/05/09 06:00
PHST- 2014/12/03 00:00 [received]
PHST- 2015/03/05 00:00 [revised]
PHST- 2015/03/23 00:00 [accepted]
PHST- 2015/05/09 06:00 [entrez]
PHST- 2015/05/09 06:00 [pubmed]
PHST- 2016/02/02 06:00 [medline]
AID - S0928-4931(15)00234-9 [pii]
AID - 10.1016/j.msec.2015.03.052 [doi]
PST - ppublish
SO  - Mater Sci Eng C Mater Biol Appl. 2015;52:259-66. doi: 10.1016/j.msec.2015.03.052. 
      Epub 2015 Mar 25.