PMID- 25953955
OWN - NLM
STAT- MEDLINE
DCOM- 20160422
LR  - 20181202
IS  - 1748-605X (Electronic)
IS  - 1748-6041 (Linking)
VI  - 10
IP  - 3
DP  - 2015 May 8
TI  - Bone regeneration and gene expression in bone defects under healthy and 
      osteoporotic bone conditions using two commercially available bone graft 
      substitutes.
PG  - 035003
LID - 10.1088/1748-6041/10/3/035003 [doi]
AB  - Biosilicate((R)) and Bio-Oss((R)) are two commercially available bone substitutes, 
      however, little is known regarding their efficacy in osteoporotic conditions. The 
      purpose of this study was to evaluate the osteogenic properties of both 
      materials, at tissue and molecular level. Thirty-six Wistar rats were submitted 
      to ovariectomy (OVX) for inducing osteoporotic conditions and sham surgery (SHAM) 
      as a control. Bone defects were created in both femurs, which were filled with 
      Biosilicate((R)) or Bio-Oss((R)), and empty defects were used as control. For the 
      healthy condition both Biosilicate((R)) and Bio-Oss((R)) did not improve bone 
      formation after 4 weeks. Histomorphometric evaluation of osteoporotic bone 
      defects with bone substitutes showed more bone formation, significant for 
      Bio-Oss((R)). Molecular biological evaluation was performed by gene-expression 
      analysis (Runx-2, ALP, OC, OPG, RANKL). The relative gene expression was 
      increased with Biosilicate((R)) for all genes in OVX rats and for Runx-2, ALP, OC 
      and RANKL in SHAM rats. In contrast, with Bio-Oss((R)), the relative gene 
      expression of OVX rats was similar for all three groups. For SHAM rats it was 
      increased for Runx-2, ALP, OC and RANKL. Since both materials improved bone 
      regeneration in osteoporotic conditions, our results suggest that bone defects in 
      osteoporotic conditions can be efficiently treated with these two bone 
      substitutes.
FAU - van Houdt, Claire I A
AU  - van Houdt CI
AD  - Department of Biomaterials, Radboudumc, 6500 HB Nijmegen, The Netherlands.
FAU - Tim, Carla R
AU  - Tim CR
FAU - Crovace, Murilo C
AU  - Crovace MC
FAU - Zanotto, Edgar D
AU  - Zanotto ED
FAU - Peitl, Oscar
AU  - Peitl O
FAU - Ulrich, Dietmar J O
AU  - Ulrich DJ
FAU - Jansen, John A
AU  - Jansen JA
FAU - Parizotto, Nivaldo A
AU  - Parizotto NA
FAU - Renno, Ana C
AU  - Renno AC
FAU - van den Beucken, Jeroen J J P
AU  - van den Beucken JJ
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150508
PL  - England
TA  - Biomed Mater
JT  - Biomedical materials (Bristol, England)
JID - 101285195
RN  - 0 (Bio-Oss)
RN  - 0 (Bone Substitutes)
RN  - 0 (Core Binding Factor Alpha 1 Subunit)
RN  - 0 (Minerals)
RN  - 0 (Osteoprotegerin)
RN  - 0 (RANK Ligand)
RN  - 0 (Runx2 protein, rat)
RN  - 0 (Tnfrsf11b protein, rat)
RN  - 0 (sodium oxide-calcium oxide-silicon dioxide-diphosphorus pentoxide)
RN  - 104982-03-8 (Osteocalcin)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
SB  - IM
MH  - Alkaline Phosphatase/genetics
MH  - Animals
MH  - *Bone Regeneration/genetics/physiology
MH  - *Bone Substitutes/chemistry
MH  - Cells, Cultured
MH  - Core Binding Factor Alpha 1 Subunit/genetics
MH  - Disease Models, Animal
MH  - Female
MH  - Gene Expression
MH  - Glass/chemistry
MH  - Materials Testing
MH  - Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/cytology
MH  - Minerals/chemistry
MH  - Osteocalcin/genetics
MH  - Osteoporosis/genetics/pathology/*therapy
MH  - Osteoprotegerin/genetics
MH  - RANK Ligand/genetics
MH  - Rats
MH  - Rats, Wistar
MH  - Tissue Engineering
MH  - Tissue Scaffolds/chemistry
EDAT- 2015/05/09 06:00
MHDA- 2016/04/23 06:00
CRDT- 2015/05/09 06:00
PHST- 2015/05/09 06:00 [entrez]
PHST- 2015/05/09 06:00 [pubmed]
PHST- 2016/04/23 06:00 [medline]
AID - 10.1088/1748-6041/10/3/035003 [doi]
PST - epublish
SO  - Biomed Mater. 2015 May 8;10(3):035003. doi: 10.1088/1748-6041/10/3/035003.