PMID- 25955291
OWN - NLM
STAT- MEDLINE
DCOM- 20160304
LR  - 20181202
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Print)
IS  - 1791-2997 (Linking)
VI  - 12
IP  - 2
DP  - 2015 Aug
TI  - Lipopolysaccharide pretreatment inhibits LPS-induced human umbilical cord 
      mesenchymal stem cell apoptosis via upregulating the expression of cellular 
      FLICE-inhibitory protein.
PG  - 2521-8
LID - 10.3892/mmr.2015.3723 [doi]
AB  - Mesenchymal stem cell (MSC)-based regenerative therapy is currently regarded as a 
      novel approach with which to repair damaged tissues. However, the efficiency of 
      MSC transplantation is limited due to the low survival rate of engrafted MSCs. 
      Lipopolysaccharide (LPS) production is increased in numerous diseases and serves 
      an essential function in the regulation of apoptosis in a variety of cell types. 
      Previous studies have indicated that low-dose LPS pretreatment contributes to 
      cytoprotection. In the current study, LPS was demonstrated to induce apoptosis in 
      human umbilical cord mesenchymal stem cells (hUCMSCs) via the activation of 
      caspase, in a dose-dependent manner. Low-dose LPS pretreatment may protect 
      hUCMSCs against apoptosis induced by high-dose LPS, by upregulating the 
      expression of cellular FADD-like IL-1beta-converting enzyme-inhibitory protein 
      (c-FLIP). The results of the present study indicate that pretreatment with an 
      appropriate concentration of LPS may alleviate high-dose LPS-induced apoptosis.
FAU - Hou, Yu Sen
AU  - Hou YS
AD  - Department of Burns and Plastic Surgery, The First Affiliated Hospital of PLA 
      General Hospital, Beijing 100048, P.R. China.
FAU - Liu, Ling Ying
AU  - Liu LY
AD  - Department of Burns and Plastic Surgery, The First Affiliated Hospital of PLA 
      General Hospital, Beijing 100048, P.R. China.
FAU - Chai, Jia Ke
AU  - Chai JK
AD  - Department of Burns and Plastic Surgery, The First Affiliated Hospital of PLA 
      General Hospital, Beijing 100048, P.R. China.
FAU - Yu, Yong Hui
AU  - Yu YH
AD  - Department of Burns and Plastic Surgery, The First Affiliated Hospital of PLA 
      General Hospital, Beijing 100048, P.R. China.
FAU - Duan, Hong Jie
AU  - Duan HJ
AD  - Department of Burns and Plastic Surgery, The First Affiliated Hospital of PLA 
      General Hospital, Beijing 100048, P.R. China.
FAU - Hu, Quan
AU  - Hu Q
AD  - Department of Burns and Plastic Surgery, The First Affiliated Hospital of PLA 
      General Hospital, Beijing 100048, P.R. China.
FAU - Yin, Hui Nan
AU  - Yin HN
AD  - Department of Burns and Plastic Surgery, The First Affiliated Hospital of PLA 
      General Hospital, Beijing 100048, P.R. China.
FAU - Wang, Yi He
AU  - Wang YH
AD  - Department of Burns and Plastic Surgery, The First Affiliated Hospital of PLA 
      General Hospital, Beijing 100048, P.R. China.
FAU - Zhuang, Shu Bo
AU  - Zhuang SB
AD  - Department of Burns and Plastic Surgery, The First Affiliated Hospital of PLA 
      General Hospital, Beijing 100048, P.R. China.
FAU - Fan, Jun
AU  - Fan J
AD  - Department of Burns and Plastic Surgery, The First Affiliated Hospital of PLA 
      General Hospital, Beijing 100048, P.R. China.
FAU - Chu, Wan Li
AU  - Chu WL
AD  - Department of Burns and Plastic Surgery, The First Affiliated Hospital of PLA 
      General Hospital, Beijing 100048, P.R. China.
FAU - Ma, Li
AU  - Ma L
AD  - Department of Burns and Plastic Surgery, The First Affiliated Hospital of PLA 
      General Hospital, Beijing 100048, P.R. China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150504
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (CASP8 and FADD-Like Apoptosis Regulating Protein)
RN  - 0 (CFLAR protein, human)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (RNA, Small Interfering)
RN  - EC 3.4.22.- (CASP8 protein, human)
RN  - EC 3.4.22.- (Caspase 3)
RN  - EC 3.4.22.- (Caspase 8)
SB  - IM
MH  - Apoptosis/drug effects
MH  - CASP8 and FADD-Like Apoptosis Regulating Protein/agonists/antagonists & 
      inhibitors/genetics/*immunology
MH  - Caspase 3/genetics/immunology
MH  - Caspase 8/genetics/immunology
MH  - Cell Survival/drug effects
MH  - Cytoprotection
MH  - Fetal Blood/cytology/drug effects/immunology
MH  - Gene Expression Regulation
MH  - Hormesis/*immunology
MH  - Humans
MH  - Lipopolysaccharides/*pharmacology
MH  - Mesenchymal Stem Cells/cytology/*drug effects/immunology
MH  - Primary Cell Culture
MH  - RNA, Small Interfering/genetics/immunology
MH  - Signal Transduction
PMC - PMC4464426
EDAT- 2015/05/09 06:00
MHDA- 2016/03/05 06:00
PMCR- 2015/05/04
CRDT- 2015/05/09 06:00
PHST- 2013/12/05 00:00 [received]
PHST- 2015/04/10 00:00 [accepted]
PHST- 2015/05/09 06:00 [entrez]
PHST- 2015/05/09 06:00 [pubmed]
PHST- 2016/03/05 06:00 [medline]
PHST- 2015/05/04 00:00 [pmc-release]
AID - mmr-12-02-2521 [pii]
AID - 10.3892/mmr.2015.3723 [doi]
PST - ppublish
SO  - Mol Med Rep. 2015 Aug;12(2):2521-8. doi: 10.3892/mmr.2015.3723. Epub 2015 May 4.