PMID- 25955435
OWN - NLM
STAT- MEDLINE
DCOM- 20160316
LR  - 20180816
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Linking)
VI  - 12
IP  - 2
DP  - 2015 Aug
TI  - Brain-derived neurotrophic factor is a novel target gene of the 
      has-miR-183/96/182 cluster in retinal pigment epithelial cells following visible 
      light exposure.
PG  - 2793-9
LID - 10.3892/mmr.2015.3736 [doi]
AB  - Light-induced retinal injury is clinically and experimentally well-documented. It 
      may be categorized into three types: Photothermal, photomechanical and 
      photochemical injuries. To date, the variation in the hsa-miR-183/96/182 cluster 
      and its potential target genes in human primary retinal pigment epithelial (RPE) 
      cells, following visible light exposure, has not been reported. In the present 
      study, RPE cells were exposed to 4 h of constant visible light. The expression of 
      the hsa-miR-183/96/182 cluster was determined using reverse 
      transcription-quantitative polymerase chain reaction (RT-qPCR) and its potential 
      target genes were investigated. Additionally, hsa-miR-183, hsa-miR-96, 
      hsa-miR-182 and has-miR-183/96/182 mimics were designed and synthesized in vitro, 
      and transfected into the RPE cells. Subsequently, the expression of brain-derived 
      neurotrophic factor (BDNF) mRNA and protein was measured, using RT-qPCR and 
      western blotting, respectively. The regulation of miRNAs to the BDNF gene were 
      then validated using a dual luciferase reporter gene assay system. The expression 
      of hsa-miR-183, hsa-miR-96 and hsa-miR-182 significantly increased in RPE cells 
      following 4 h of visible light exposure, compared with RPE cells that had been 
      exposed to dark conditions (P<0.01). Following RPE cell transfection with mimics, 
      BDNF mRNA and protein expression in the RPE cells was significantly downregulated 
      compared with control RPE cells (P<0.05, P<0.01, respectively). Similarly, the 
      ratio of Renilla luciferase/firefly luciferase significantly decreased in the RPE 
      cells of the mimic + wild type (WT) group compared with cells of the 
      psiCHECK(TM)-2 (a vector lacking the sequence of the BDNF gene), wild type and 
      mimic + mutation groups (P<0.05, P<0.01). The present study suggests that BDNF is 
      a target gene of the has-miR-183-96-182 cluster in RPE cells. The present study 
      suggests an underlying protective mechanism against retinal light injury and may 
      provide a novel target for the prevention and treatment of light-induced retinal 
      injury.
FAU - Li, Hongyang
AU  - Li H
AD  - Department of Opthalmology, The Chinese People's Liberation Army General 
      Hospital, Beijing 100000, P.R. China.
FAU - Gong, Yan
AU  - Gong Y
AD  - Department of Opthalmology, The Chinese People's Liberation Army General 
      Hospital, Beijing 100000, P.R. China.
FAU - Qian, Haiyan
AU  - Qian H
AD  - Department of Opthalmology, The Chinese People's Liberation Army General 
      Hospital, Beijing 100000, P.R. China.
FAU - Chen, Tingjun
AU  - Chen T
AD  - Department of Opthalmology, The Chinese People's Liberation Army General 
      Hospital, Beijing 100000, P.R. China.
FAU - Liu, Zihao
AU  - Liu Z
AD  - Department of Opthalmology, The Chinese People's Liberation Army General 
      Hospital, Beijing 100000, P.R. China.
FAU - Jiang, Zhaocai
AU  - Jiang Z
AD  - Department of Opthalmology, The Chinese People's Liberation Army General 
      Hospital, Beijing 100000, P.R. China.
FAU - Wei, Shihui
AU  - Wei S
AD  - Department of Opthalmology, The Chinese People's Liberation Army General 
      Hospital, Beijing 100000, P.R. China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150506
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (MIRN183 microRNA, human)
RN  - 0 (MIRN96 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (Mirn182 microRNA, human)
RN  - 0 (Oligonucleotides, Antisense)
RN  - 0 (RNA, Messenger)
RN  - 7171WSG8A2 (BDNF protein, human)
SB  - IM
MH  - Brain-Derived Neurotrophic Factor/genetics/*metabolism
MH  - Cells, Cultured
MH  - Down-Regulation
MH  - Humans
MH  - *Light
MH  - MicroRNAs/antagonists & inhibitors/genetics/*metabolism
MH  - Multigene Family
MH  - Oligonucleotides, Antisense/metabolism
MH  - RNA, Messenger
MH  - Real-Time Polymerase Chain Reaction
MH  - Retinal Pigment Epithelium/cytology/metabolism/*radiation effects
EDAT- 2015/05/09 06:00
MHDA- 2016/03/17 06:00
CRDT- 2015/05/09 06:00
PHST- 2014/03/14 00:00 [received]
PHST- 2014/12/09 00:00 [accepted]
PHST- 2015/05/09 06:00 [entrez]
PHST- 2015/05/09 06:00 [pubmed]
PHST- 2016/03/17 06:00 [medline]
AID - 10.3892/mmr.2015.3736 [doi]
PST - ppublish
SO  - Mol Med Rep. 2015 Aug;12(2):2793-9. doi: 10.3892/mmr.2015.3736. Epub 2015 May 6.