PMID- 25957883
OWN - NLM
STAT- MEDLINE
DCOM- 20160404
LR  - 20150711
IS  - 1095-9947 (Electronic)
IS  - 1050-4648 (Linking)
VI  - 45
IP  - 2
DP  - 2015 Aug
TI  - Immobilization antigen vaccine adjuvanted by parasitic heat shock protein 70C 
      confers high protection in fish against cryptocaryonosis.
PG  - 517-27
LID - S1050-4648(15)00198-9 [pii]
LID - 10.1016/j.fsi.2015.04.036 [doi]
AB  - The immobilization antigen (iAg) has been demonstrated as a protective immunogen 
      against Cryptocaryon irritans infection. In this study, C-terminal domain of heat 
      shock protein 70 cloned from C. irritans (Hsp70C) was tested for its 
      immuno-stimulatory effects. The iAg and Hsp70C cDNAs were constructed 
      independently in secretory forms and were encapsulated in chitosan nanoparticles. 
      In the first immunization trial, grouper fingerlings orally intubated with iAg 
      and iAg:Hsp70C presented 96% and 100% relative percent survival (RPS), 
      respectively, after a lethal challenge. In the second trial, both iAg and 
      iAg:Hsp70C groups showed 100% RPS and the skin trophont burden was significantly 
      lowered. The iAg:Hsp70C still provides a significantly high protection of 51% RPS 
      at 49 days post immunization, when an even more serious lethal infection occurs. 
      RT-qPCR results showed that Hsp70C could up-regulate the expression of i) T cell 
      markers: Cluster of Differentiation 8 alpha (CD8alpha) and CD4, ii) cytokine genes: 
      Interferon gamma (IFNgamma), Tumor Necrosis Factor alpha (TNFalpha) and Interleukin 12 
      p40 (IL-12/P40), iii) antibody genes: Immunoglobulin M heavy chain (IgMH) and 
      IgTH, and iv) major histocompatibility complex (MHC-I & MHC-II), in the spleen of 
      iAg:Hsp70C group. Furthermore, significantly high levels of iAg-specific IgM was 
      detected in skin mucus which efficiently immobilized live theronts in iAg- and 
      iAg:Hsp70C-immunized fish at 5 weeks post immunization. Hsp70C significantly 
      increased the number of nonspecific CD8(+) skin leucocytes which exerted 
      cytotoxicity against theronts, although cytotoxic activity showed no difference 
      among the various groups. Because of this complementary cooperation of cellular 
      and humoral immune responses, Hsp70C enhances the efficacy of iAg vaccine and 
      constrains C. irritans infection. In view of the severe loss caused by 
      cryptocaryonosis, application of this parasitic vaccine in farmed and ornamental 
      fish, is worthy to be considered.
CI  - Copyright (c) 2015 Elsevier Ltd. All rights reserved.
FAU - Josepriya, T A
AU  - Josepriya TA
AD  - Department of Life Science, National Taiwan University, Taipei 106, Taiwan.
FAU - Chien, Kuo-Hsuan
AU  - Chien KH
AD  - Department of Life Science, National Taiwan University, Taipei 106, Taiwan.
FAU - Lin, Hsin-Yun
AU  - Lin HY
AD  - Department of Life Science, National Taiwan University, Taipei 106, Taiwan.
FAU - Huang, Han-Ning
AU  - Huang HN
AD  - Department of Food Science, National Taiwan Ocean University, Keelung, Taiwan; 
      Center for Marine Bioscience and Biotechnology, National Taiwan Ocean University, 
      Keelung, Taiwan.
FAU - Wu, Chang-Jer
AU  - Wu CJ
AD  - Department of Food Science, National Taiwan Ocean University, Keelung, Taiwan.
FAU - Song, Yen-Ling
AU  - Song YL
AD  - Department of Life Science, National Taiwan University, Taipei 106, Taiwan. 
      Electronic address: song@ntu.edu.tw.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150507
PL  - England
TA  - Fish Shellfish Immunol
JT  - Fish & shellfish immunology
JID - 9505220
RN  - 0 (Antigens, Protozoan)
RN  - 0 (HSP70 Heat-Shock Proteins)
RN  - 0 (Immunoglobulin M)
RN  - 0 (Protozoan Proteins)
RN  - 0 (Protozoan Vaccines)
RN  - 0 (immobilization antigens)
SB  - IM
MH  - Animals
MH  - Antigens, Protozoan/administration & dosage/*immunology
MH  - CD8-Positive T-Lymphocytes/immunology
MH  - Ciliophora/immunology
MH  - Ciliophora Infections/immunology/*prevention & control
MH  - Disease Resistance
MH  - Fish Diseases/immunology/*prevention & control
MH  - HSP70 Heat-Shock Proteins/administration & dosage/genetics/*immunology
MH  - Immunoglobulin M/immunology
MH  - Nanoparticles/administration & dosage
MH  - Perciformes
MH  - Protozoan Proteins/administration & dosage/*immunology
MH  - Protozoan Vaccines/*administration & dosage
MH  - Skin/immunology
MH  - Tetrahymena thermophila/genetics
OTO - NOTNLM
OT  - Cryptocaryon irritans
OT  - DNA vaccine
OT  - Epinephelus coioides
OT  - Mucosal IgM
OT  - Parasitic Hsp70C
OT  - Parasitic iAg
OT  - Skin CD8(+) cells
EDAT- 2015/05/11 06:00
MHDA- 2016/04/05 06:00
CRDT- 2015/05/11 06:00
PHST- 2015/02/05 00:00 [received]
PHST- 2015/04/18 00:00 [revised]
PHST- 2015/04/27 00:00 [accepted]
PHST- 2015/05/11 06:00 [entrez]
PHST- 2015/05/11 06:00 [pubmed]
PHST- 2016/04/05 06:00 [medline]
AID - S1050-4648(15)00198-9 [pii]
AID - 10.1016/j.fsi.2015.04.036 [doi]
PST - ppublish
SO  - Fish Shellfish Immunol. 2015 Aug;45(2):517-27. doi: 10.1016/j.fsi.2015.04.036. 
      Epub 2015 May 7.