PMID- 25957946
OWN - NLM
STAT- MEDLINE
DCOM- 20160425
LR  - 20181113
IS  - 1876-7753 (Electronic)
IS  - 1873-5061 (Print)
IS  - 1873-5061 (Linking)
VI  - 15
IP  - 1
DP  - 2015 Jul
TI  - Chemoattraction of bone marrow-derived stem cells towards human endometrial 
      stromal cells is mediated by estradiol regulated CXCL12 and CXCR4 expression.
PG  - 14-22
LID - S1873-5061(15)00046-X [pii]
LID - 10.1016/j.scr.2015.04.004 [doi]
AB  - Bone marrow derived cells engraft to the uterine endometrium and contribute to 
      endometriosis. The mechanism by which these cells are mobilized and directed to 
      the endometrium has not been previously characterized. We demonstrate that human 
      endometrial stromal cells (hESCs) produce the chemokine CXCL12 and that bone 
      marrow cells (BMCs) express the CXCL12 receptor, CXCR4. Treatment with 
      physiological levels of estradiol (E2) induced both CXCL12 and CXCR4 expression 
      in hESCs and BMCs, respectively. BMCs migrated towards hESCs conditioned media; a 
      CXCR4 antagonist blocked migration indicating that CXCL12 acting through its 
      receptor, CXCR4, is necessary for chemoattraction of BM cells to human 
      endometrial cells. E2 increased both CXCL12 expression in endometrial cells and 
      CXCR4 expression in BM cells, further enhancing chemoattraction. E2 induced 
      CXCL12/CXCR4 expression in endometrium and BM, respectively, drives migration of 
      stem cells to the endometrium. The E2-CXCL12/CXCR4 signaling pathway may be 
      useful in determining treatments for endometrial disorders, and may be 
      antagonized to block stem cell migration to endometriosis.
CI  - Copyright (c) 2015. Published by Elsevier B.V.
FAU - Wang, Xiuli
AU  - Wang X
AD  - Department of Obstetrics, Gynecology and Reproductive Sciences, Yale School of 
      Medicine, New Haven, CT, USA.
FAU - Mamillapalli, Ramanaiah
AU  - Mamillapalli R
AD  - Department of Obstetrics, Gynecology and Reproductive Sciences, Yale School of 
      Medicine, New Haven, CT, USA. Electronic address: ramana.mamillapalli@yale.edu.
FAU - Mutlu, Levent
AU  - Mutlu L
AD  - Department of Obstetrics, Gynecology and Reproductive Sciences, Yale School of 
      Medicine, New Haven, CT, USA.
FAU - Du, Hongling
AU  - Du H
AD  - Department of Obstetrics, Gynecology and Reproductive Sciences, Yale School of 
      Medicine, New Haven, CT, USA.
FAU - Taylor, Hugh S
AU  - Taylor HS
AD  - Department of Obstetrics, Gynecology and Reproductive Sciences, Yale School of 
      Medicine, New Haven, CT, USA.
LA  - eng
GR  - R01 HD076422/HD/NICHD NIH HHS/United States
PT  - Journal Article
DEP - 20150424
PL  - England
TA  - Stem Cell Res
JT  - Stem cell research
JID - 101316957
RN  - 0 (Chemokine CXCL12)
RN  - 0 (Chemotactic Factors)
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Receptors, CXCR4)
RN  - 4G7DS2Q64Y (Progesterone)
RN  - 4TI98Z838E (Estradiol)
RN  - EC 3.1.3.48 (Leukocyte Common Antigens)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Bone Marrow Cells/*cytology
MH  - Chemokine CXCL12/*metabolism
MH  - Chemotactic Factors/*pharmacology
MH  - Chemotaxis/drug effects
MH  - Culture Media, Conditioned/pharmacology
MH  - Endometrium/*cytology
MH  - Estradiol/*pharmacology
MH  - Female
MH  - Flow Cytometry
MH  - Humans
MH  - Immunophenotyping
MH  - Leukocyte Common Antigens/metabolism
MH  - Mice, Inbred C57BL
MH  - Progesterone/pharmacology
MH  - Receptors, CXCR4/*metabolism
MH  - Stem Cells/*cytology/drug effects/metabolism
MH  - Stromal Cells
MH  - Uterus/cytology
PMC - PMC5001152
MID - NIHMS809416
EDAT- 2015/05/11 06:00
MHDA- 2016/04/26 06:00
PMCR- 2016/08/26
CRDT- 2015/05/11 06:00
PHST- 2015/01/14 00:00 [received]
PHST- 2015/04/04 00:00 [revised]
PHST- 2015/04/18 00:00 [accepted]
PHST- 2015/05/11 06:00 [entrez]
PHST- 2015/05/11 06:00 [pubmed]
PHST- 2016/04/26 06:00 [medline]
PHST- 2016/08/26 00:00 [pmc-release]
AID - S1873-5061(15)00046-X [pii]
AID - 10.1016/j.scr.2015.04.004 [doi]
PST - ppublish
SO  - Stem Cell Res. 2015 Jul;15(1):14-22. doi: 10.1016/j.scr.2015.04.004. Epub 2015 
      Apr 24.