PMID- 25957965
OWN - NLM
STAT- MEDLINE
DCOM- 20151201
LR  - 20181113
IS  - 1537-6613 (Electronic)
IS  - 0022-1899 (Print)
IS  - 0022-1899 (Linking)
VI  - 212 Suppl 2
IP  - Suppl 2
DP  - 2015 Oct 1
TI  - Soluble Glycoprotein Is Not Required for Ebola Virus Virulence in Guinea Pigs.
PG  - S242-6
LID - 10.1093/infdis/jiv111 [doi]
AB  - Ebola virus (EBOV) uses transcriptional editing to express several glycoproteins 
      (GPs), including secreted soluble GP (sGP) and structural GP1,2, from a single 
      gene. Recombinant viruses predominantly expressing GP1,2 are known to rapidly 
      mutate and acquire an editing site predominantly expressing sGP in vivo, 
      suggesting an important role of this protein during infection. Therefore, we 
      generated a recombinant virus that is no longer able to express sGP and assessed 
      its virulence in the EBOV guinea pig model. Surprisingly, although this virus 
      remained genetically stable, it did not show any significant attenuation in vivo, 
      showing that sGP is not required for virulence in this model.
CI  - Published by Oxford University Press on behalf of the Infectious Diseases Society 
      of America 2015. This work is written by (a) US Government employee(s) and is in 
      the public domain in the US.
FAU - Hoenen, Thomas
AU  - Hoenen T
AD  - Laboratory of Virology.
FAU - Marzi, Andrea
AU  - Marzi A
AD  - Laboratory of Virology.
FAU - Scott, Dana P
AU  - Scott DP
AD  - Rocky Mountain Veterinary Branch, Division of Intramural Research, National 
      Institute of Allergy and Infectious Diseases, National Institutes of Health, 
      Hamilton, Montana.
FAU - Feldmann, Friederike
AU  - Feldmann F
AD  - Rocky Mountain Veterinary Branch, Division of Intramural Research, National 
      Institute of Allergy and Infectious Diseases, National Institutes of Health, 
      Hamilton, Montana.
FAU - Callison, Julie
AU  - Callison J
AD  - Laboratory of Virology.
FAU - Safronetz, David
AU  - Safronetz D
AD  - Laboratory of Virology.
FAU - Ebihara, Hideki
AU  - Ebihara H
AD  - Laboratory of Virology.
FAU - Feldmann, Heinz
AU  - Feldmann H
AD  - Laboratory of Virology.
LA  - eng
GR  - Intramural NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Intramural
DEP - 20150509
PL  - United States
TA  - J Infect Dis
JT  - The Journal of infectious diseases
JID - 0413675
RN  - 0 (Glycoproteins)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Animals
MH  - Ebolavirus/*genetics/*pathogenicity
MH  - Female
MH  - Gene Expression Regulation, Viral/genetics
MH  - Glycoproteins/*genetics
MH  - Guinea Pigs
MH  - Hemorrhagic Fever, Ebola/*virology
MH  - Viral Proteins/*genetics
MH  - Virulence/*genetics
PMC - PMC4564536
OTO - NOTNLM
OT  - ebola virus
OT  - guinea pigs
OT  - mRNA editing
OT  - recombinant virus
OT  - reverse genetics
OT  - sGP
OT  - soluble glycoprotein
OT  - virulence
EDAT- 2015/05/11 06:00
MHDA- 2015/12/15 06:00
PMCR- 2016/10/01
CRDT- 2015/05/11 06:00
PHST- 2015/05/11 06:00 [entrez]
PHST- 2015/05/11 06:00 [pubmed]
PHST- 2015/12/15 06:00 [medline]
PHST- 2016/10/01 00:00 [pmc-release]
AID - jiv111 [pii]
AID - 10.1093/infdis/jiv111 [doi]
PST - ppublish
SO  - J Infect Dis. 2015 Oct 1;212 Suppl 2(Suppl 2):S242-6. doi: 10.1093/infdis/jiv111. 
      Epub 2015 May 9.