PMID- 25958180
OWN - NLM
STAT- MEDLINE
DCOM- 20160324
LR  - 20240323
IS  - 1573-6857 (Electronic)
IS  - 0016-6707 (Print)
IS  - 0016-6707 (Linking)
VI  - 143
IP  - 4
DP  - 2015 Aug
TI  - Chromosomal characteristics and distribution of rDNA sequences in the brook trout 
      Salvelinus fontinalis (Mitchill, 1814).
PG  - 425-32
LID - 10.1007/s10709-015-9841-6 [doi]
AB  - Brook trout Salvelinus fontinalis (Mitchill, 1814) chromosomes have been analyzed 
      using conventional and molecular cytogenetic techniques enabling characteristics 
      and chromosomal location of heterochromatin, nucleolus organizer regions (NORs), 
      ribosomal RNA-encoding genes and telomeric DNA sequences. The C-banding and 
      chromosome digestion with the restriction endonucleases demonstrated distribution 
      and heterogeneity of the heterochromatin in the brook trout genome. DNA sequences 
      of the ribosomal RNA genes, namely the nucleolus-forming 28S (major) and 
      non-nucleolus-forming 5S (minor) rDNAs, were physically mapped using fluorescence 
      in situ hybridization (FISH) and primed in situ labelling. The minor rDNA locus 
      was located on the subtelo-acrocentric chromosome pair No. 9, whereas the major 
      rDNA loci were dispersed on 14 chromosome pairs, showing a considerable 
      inter-individual variation in the number and location. The major and minor rDNA 
      loci were located at different chromosomes. Multichromosomal location (3-6 sites) 
      of the NORs was demonstrated by silver nitrate (AgNO3) impregnation. All 
      Ag-positive i.e. active NORs corresponded to the GC-rich blocks of 
      heterochromatin. FISH with telomeric probe showed the presence of the 
      interstitial telomeric site (ITS) adjacent to the NOR/28S rDNA site on the 
      chromosome 11. This ITS was presumably remnant of the chromosome rearrangement(s) 
      leading to the genomic redistribution of the rDNA sequences. Comparative analysis 
      of the cytogenetic data among several related salmonid species confirmed huge 
      variation in the number and the chromosomal location of rRNA gene clusters in the 
      Salvelinus genome.
FAU - Sliwinska-Jewsiewicka, A
AU  - Sliwinska-Jewsiewicka A
AD  - Department of Human Histology and Embryology, Faculty of Medical Sciences, 
      University of Warmia and Mazury in Olsztyn, Olsztyn, Poland.
FAU - Kucinski, M
AU  - Kucinski M
FAU - Kirtiklis, L
AU  - Kirtiklis L
FAU - Dobosz, S
AU  - Dobosz S
FAU - Ocalewicz, K
AU  - Ocalewicz K
FAU - Jankun, Malgorzata
AU  - Jankun M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150510
PL  - Netherlands
TA  - Genetica
JT  - Genetica
JID - 0370740
RN  - 0 (DNA, Ribosomal)
SB  - IM
MH  - Animals
MH  - Chromosome Banding
MH  - *Chromosome Mapping
MH  - *DNA, Ribosomal
MH  - Female
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Male
MH  - Trout/*genetics
PMC - PMC4486110
EDAT- 2015/05/11 06:00
MHDA- 2016/03/25 06:00
PMCR- 2015/05/10
CRDT- 2015/05/11 06:00
PHST- 2015/03/11 00:00 [received]
PHST- 2015/05/04 00:00 [accepted]
PHST- 2015/05/11 06:00 [entrez]
PHST- 2015/05/11 06:00 [pubmed]
PHST- 2016/03/25 06:00 [medline]
PHST- 2015/05/10 00:00 [pmc-release]
AID - 9841 [pii]
AID - 10.1007/s10709-015-9841-6 [doi]
PST - ppublish
SO  - Genetica. 2015 Aug;143(4):425-32. doi: 10.1007/s10709-015-9841-6. Epub 2015 May 
      10.