PMID- 25958920
OWN - NLM
STAT- MEDLINE
DCOM- 20170822
LR  - 20181202
IS  - 1462-2920 (Electronic)
IS  - 1462-2912 (Linking)
VI  - 18
IP  - 5
DP  - 2016 May
TI  - Exposure to soil, house dust and decaying plants increases gut microbial 
      diversity and decreases serum immunoglobulin E levels in BALB/c mice.
PG  - 1326-37
LID - 10.1111/1462-2920.12895 [doi]
AB  - To assess the impact of sanitation of a living environment on gut microbiota and 
      development of the immune system, we raised BALB/c mice under three distinct 
      environmental conditions: a specific pathogen-free animal room (SPF), a general 
      animal room (XZ) and a farmhouse (JD). All other variables like diet, age, 
      genetic background, physiological status and original gut microbiota were 
      controlled for in the three groups. Using high-throughput sequencing of the 16S 
      rRNA gene, we found that each mouse group had a specific structure of the gut 
      microbial community. Groups JD and XZ harboured a significantly more diverse and 
      richer gut microbiota than did group SPF. Bacteroidetes were significantly more 
      abundant in groups XZ and JD than in group SPF, whereas Firmicutes showed the 
      inverse pattern. Total serum immunoglobulin E (IgE) levels were significantly 
      lower in groups XZ and JD than in group SPF. There were no significant 
      differences in gut microbiota diversity and serum IgE concentration between 
      groups JD and XZ, but we found higher abundance of dominant genera in the gut 
      microflora of group JD. We conclude that exposure to soil, house dust and 
      decaying plant material enhances gut microbial diversity and innate immunity. Our 
      results seem to provide new evidence supporting the hygiene hypothesis.
CI  - (c) 2015 Society for Applied Microbiology and John Wiley & Sons Ltd.
FAU - Zhou, Dongrui
AU  - Zhou D
AD  - Key Laboratory of Child Development and Learning Sciences, Southeast University, 
      Nanjing, 210096, China.
AD  - Suzhou Research Institute of Southeast University, Suzhou, 215123, China.
FAU - Zhang, Honglin
AU  - Zhang H
AD  - State Key Laboratory of Bioelectronics, Southeast University, Nanjing, 210096, 
      China.
AD  - College of Food Science, Nanjing Xiaozhuang University, Nanjing, 211171, China.
FAU - Bai, Zhimao
AU  - Bai Z
AD  - School of Public Health, Southeast University, Nanjing, 210096, China.
FAU - Zhang, Aidi
AU  - Zhang A
AD  - Key Laboratory of Child Development and Learning Sciences, Southeast University, 
      Nanjing, 210096, China.
FAU - Bai, Futian
AU  - Bai F
AD  - Medical School, Southeast University, Nanjing, 210096, China.
FAU - Luo, Xing
AU  - Luo X
AD  - State Key Laboratory of Bioelectronics, Southeast University, Nanjing, 210096, 
      China.
FAU - Hou, Yue
AU  - Hou Y
AD  - State Key Laboratory of Bioelectronics, Southeast University, Nanjing, 210096, 
      China.
FAU - Ding, Xiao
AU  - Ding X
AD  - State Key Laboratory of Bioelectronics, Southeast University, Nanjing, 210096, 
      China.
FAU - Sun, Beili
AU  - Sun B
AD  - State Key Laboratory of Bioelectronics, Southeast University, Nanjing, 210096, 
      China.
FAU - Sun, Xiao
AU  - Sun X
AD  - State Key Laboratory of Bioelectronics, Southeast University, Nanjing, 210096, 
      China.
FAU - Ma, Ning
AU  - Ma N
AD  - Key Laboratory of Child Development and Learning Sciences, Southeast University, 
      Nanjing, 210096, China.
FAU - Wang, Cuifen
AU  - Wang C
AD  - Center for Diagnostic Nanosystems, Marshall University, Huntington, WV, 25755, 
      USA.
AD  - School of Pharmacy, Marshall University, Huntington, WV, 25755, USA.
FAU - Dai, Xiaoniu
AU  - Dai X
AD  - Medical School, Southeast University, Nanjing, 210096, China.
FAU - Lu, Zuhong
AU  - Lu Z
AD  - State Key Laboratory of Bioelectronics, Southeast University, Nanjing, 210096, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20150625
PL  - England
TA  - Environ Microbiol
JT  - Environmental microbiology
JID - 100883692
RN  - 0 (Dust)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Soil)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Animals
MH  - Bacteroidetes/genetics/isolation & purification
MH  - Biodiversity
MH  - *Dust
MH  - Female
MH  - Firmicutes/genetics/isolation & purification
MH  - *Gastrointestinal Microbiome
MH  - Gastrointestinal Tract/microbiology
MH  - Immunoglobulin E/*blood
MH  - Male
MH  - Mice, Inbred BALB C
MH  - Plants
MH  - RNA, Ribosomal, 16S/genetics
MH  - *Soil
MH  - Specific Pathogen-Free Organisms
EDAT- 2015/05/12 06:00
MHDA- 2017/08/23 06:00
CRDT- 2015/05/12 06:00
PHST- 2014/10/03 00:00 [received]
PHST- 2015/05/01 00:00 [revised]
PHST- 2015/05/01 00:00 [accepted]
PHST- 2015/05/12 06:00 [entrez]
PHST- 2015/05/12 06:00 [pubmed]
PHST- 2017/08/23 06:00 [medline]
AID - 10.1111/1462-2920.12895 [doi]
PST - ppublish
SO  - Environ Microbiol. 2016 May;18(5):1326-37. doi: 10.1111/1462-2920.12895. Epub 
      2015 Jun 25.