PMID- 25960207
OWN - NLM
STAT- MEDLINE
DCOM- 20160210
LR  - 20150511
IS  - 1950-6007 (Electronic)
IS  - 0753-3322 (Linking)
VI  - 71
DP  - 2015 Apr
TI  - Effects of miRNA-197 overexpression on proliferation, apoptosis and migration in 
      levonorgestrel treated uterine leiomyoma cells.
PG  - 1-6
LID - S0753-3322(15)00053-0 [pii]
LID - 10.1016/j.biopha.2015.02.004 [doi]
AB  - BACKGROUND/AIMS: Uterine leiomyoma is the ahead benign tumor of the female 
      genital tract, which resulted in menstrual abnormalities, recurrent pregnancy 
      loss, and other serious gynecological disorders in women. Recently, as the 
      process of exploring the brief molecular mechanisms of tumorgenesis, microRNAs 
      (miRNAs) have attracted much more attention. METHODS: In this study, we first 
      confirmed that microRNA-197 (miR-197) was down-regulated significantly in human 
      uterus leiomyoma by quantity real-time polymerase chain reaction, compared to 
      normal uterus myometrium. Then we observed the potential effects of miR-197 
      overexpression on human uterus leiomyoma cells by cell counting kit 8, wound 
      healing assay, and flow cytometric assessment separately. RESULTS: The data 
      showed that miR-197 could inhibit cell proliferation, induce cell apoptosis, and 
      block cell migration in vitro. Coincidently, levonorgestrel (LNG), a well-known 
      uterus leiomyoma therapy, could induce miR-197 expression in human uterus 
      leiomyoma cells, and over-expression of miR-197 showed a synergy effect on human 
      uterus leiomyoma cell proliferation and apoptosis with LNG. CONCLUSION: In this 
      study, the data showed that miR-197 could play an anti-oncogenic role in human 
      uterus leiomyoma cells, and cooperate with LNG on the cell proliferation and 
      apoptosis, which suggested that miR-197 might be a potential target and provided 
      database for clinical treatment.
CI  - Copyright (c) 2015 Elsevier Masson SAS. All rights reserved.
FAU - Wu, Xiaoli
AU  - Wu X
AD  - Department of Women Health Care, Nanjing Maternal and Child Health Care Hospital 
      Affiliated to Nanjing Medical University, Nanjing, Jiangsu 210004, China.
FAU - Ling, Jing
AU  - Ling J
AD  - Department of Obstetrics and Gynecology, Affiliated Jiangyin Hospital of 
      South-East University, Jiangyin 214400, China.
FAU - Fu, Ziyi
AU  - Fu Z
AD  - Nanjing Maternal and Child Health Medical Institute, Nanjing Maternity and Child 
      Health Care Hospital Affiliated to Nanjing Medical University, Nanjing, Jiangsu 
      210004, China.
FAU - Ji, Chenbo
AU  - Ji C
AD  - Nanjing Maternal and Child Health Medical Institute, Nanjing Maternity and Child 
      Health Care Hospital Affiliated to Nanjing Medical University, Nanjing, Jiangsu 
      210004, China.
FAU - Wu, Jiangping
AU  - Wu J
AD  - Department of Women Health Care, Nanjing Maternal and Child Health Care Hospital 
      Affiliated to Nanjing Medical University, Nanjing, Jiangsu 210004, China.
FAU - Xu, Qing
AU  - Xu Q
AD  - Department of Obstetrics and Gynecology, Nanjing Maternity and Child Health Care 
      Hospital Affiliated to Nanjing Medical University, Nanjing, Jiangsu 210004, 
      China. Electronic address: qingxunjmu@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150218
PL  - France
TA  - Biomed Pharmacother
JT  - Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
JID - 8213295
RN  - 0 (MIRN197 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 5W7SIA7YZW (Levonorgestrel)
SB  - IM
MH  - Adult
MH  - Apoptosis/*drug effects/genetics
MH  - Cell Movement/*drug effects/genetics
MH  - Cell Proliferation/drug effects
MH  - Down-Regulation/drug effects
MH  - Female
MH  - Gene Expression Regulation, Neoplastic/drug effects
MH  - Humans
MH  - Leiomyoma/drug therapy/*genetics/pathology
MH  - Levonorgestrel/*pharmacology/therapeutic use
MH  - MicroRNAs/*genetics/metabolism
MH  - Middle Aged
MH  - Tumor Cells, Cultured
MH  - Uterine Neoplasms/drug therapy/*genetics/pathology
OTO - NOTNLM
OT  - Apoptosis
OT  - Cell proliferation
OT  - Levonorgestrel (LNG)
OT  - Migration
OT  - Uterine leiomyoma
OT  - miRNA-197 overexpression
EDAT- 2015/05/12 06:00
MHDA- 2016/02/11 06:00
CRDT- 2015/05/12 06:00
PHST- 2015/01/15 00:00 [received]
PHST- 2015/02/09 00:00 [accepted]
PHST- 2015/05/12 06:00 [entrez]
PHST- 2015/05/12 06:00 [pubmed]
PHST- 2016/02/11 06:00 [medline]
AID - S0753-3322(15)00053-0 [pii]
AID - 10.1016/j.biopha.2015.02.004 [doi]
PST - ppublish
SO  - Biomed Pharmacother. 2015 Apr;71:1-6. doi: 10.1016/j.biopha.2015.02.004. Epub 
      2015 Feb 18.