PMID- 25962115
OWN - NLM
STAT- MEDLINE
DCOM- 20160407
LR  - 20190221
IS  - 1525-1438 (Electronic)
IS  - 1048-891X (Linking)
VI  - 25
IP  - 6
DP  - 2015 Jul
TI  - Up-Regulation of miR-204 Enhances Anoikis Sensitivity in Epithelial Ovarian 
      Cancer Cell Line Via Brain-Derived Neurotrophic Factor Pathway In Vitro.
PG  - 944-52
LID - 10.1097/IGC.0000000000000456 [doi]
AB  - OBJECTIVE: Genomic loci encoding miR-204, which was predicted to target 
      brain-derived neurotrophic factor (BDNF), were frequently lost in multiple 
      cancer, including epithelial ovarian cancer (EOC). In this study, we aimed to 
      find out the influence of miR-204 expression level on EOC cell anoikis 
      sensitivity and to explore possible mechanisms of this process. METHODS: First, 
      we screened EOC cells, which maintain anoikis resistance forming an anoikis 
      pattern. miR-204 expression level and apoptosis were measured, respectively, by 
      quantitative reverse transcriptase polymerase chain reaction and 
      Annexin-V-R-PE/7-amino-actinomycin assay. Then we restored the expression level 
      of miR-204 by transfection with pre-miR-204. miR-204 expression level and 
      apoptosis were measured as before; cell invasion and migration ability were 
      detected by transwell invasion assay and wound-healing assay. The messenger RNA 
      level of BDNF was also detected by quantitative reverse transcriptase polymerase 
      chain reaction; Western blot analysis was performed to assess pAKT expression. 
      RESULTS: Expression of miR-204 is significantly down-regulated in an anoikis 
      pattern. Restored expression level of miR-204 enables cells to acquire more 
      sensitivity to anoikis and decrease invasive and metastatic behavior, and also 
      results in BDNF down-expression and inhibits activation of mitochondria-dependent 
      pathway through the PI3K/AKT signaling pathway leading to cancer cell anoikis in 
      EOC cells. CONCLUSIONS: miR-204 up-regulation may be linked directly to the 
      sensitivity of EOC cell anoikis by contributing to BDNF down-regulation. Our 
      findings provide a novel mechanism for manipulating miR-204 levels 
      therapeutically to restore anoikis sensitivity.
FAU - Yan, Hongliang
AU  - Yan H
AD  - *Hebei United University, Tangshan, PR China; daggerDepartment of Gynaecology, the 
      Affiliated Hospital of the Chinese People's Armed Forces Logistic College, 
      Tianjin, PR China; double daggerHealthy Care Center of Women and Children of Tianjin Beichen, 
      Tianjin, PR China;  section signBiomechanics Laboratory of Orthopaedic Research Institute of 
      Tian Jin Hospital, Tianjin, China; and  parallelHebei United University, Tangshan, PR 
      China.
FAU - Wu, Weiguang
AU  - Wu W
FAU - Ge, Hongyu
AU  - Ge H
FAU - Li, Pengfei
AU  - Li P
FAU - Wang, Zheng
AU  - Wang Z
LA  - eng
PT  - Journal Article
PL  - England
TA  - Int J Gynecol Cancer
JT  - International journal of gynecological cancer : official journal of the 
      International Gynecological Cancer Society
JID - 9111626
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (MIRN204 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Small Interfering)
RN  - 7171WSG8A2 (BDNF protein, human)
SB  - IM
MH  - Anoikis/*genetics
MH  - Apoptosis
MH  - Blotting, Western
MH  - Brain-Derived Neurotrophic Factor/antagonists & inhibitors/genetics/*metabolism
MH  - Carcinoma, Ovarian Epithelial
MH  - Cell Movement
MH  - Cell Proliferation
MH  - Female
MH  - *Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - In Vitro Techniques
MH  - MicroRNAs/*genetics
MH  - Neoplasm Invasiveness
MH  - Neoplasms, Glandular and Epithelial/genetics/metabolism/*pathology
MH  - Ovarian Neoplasms/genetics/metabolism/*pathology
MH  - RNA, Messenger/genetics
MH  - RNA, Small Interfering/genetics
MH  - Real-Time Polymerase Chain Reaction
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Signal Transduction
MH  - Tumor Cells, Cultured
MH  - Up-Regulation
EDAT- 2015/05/12 06:00
MHDA- 2016/04/08 06:00
CRDT- 2015/05/12 06:00
PHST- 2015/05/12 06:00 [entrez]
PHST- 2015/05/12 06:00 [pubmed]
PHST- 2016/04/08 06:00 [medline]
AID - 10.1097/IGC.0000000000000456 [doi]
PST - ppublish
SO  - Int J Gynecol Cancer. 2015 Jul;25(6):944-52. doi: 10.1097/IGC.0000000000000456.