PMID- 25963228
OWN - NLM
STAT- MEDLINE
DCOM- 20160923
LR  - 20220414
IS  - 1744-5116 (Electronic)
IS  - 1388-0209 (Linking)
VI  - 54
IP  - 2
DP  - 2016
TI  - Cryptoporus volvatus polysaccharides attenuate LPS-induced expression of 
      pro-inflammatory factors via the TLR2 signaling pathway in human alveolar 
      epithelial cells.
PG  - 347-53
LID - 10.3109/13880209.2015.1042981 [doi]
AB  - CONTEXT: Cryptoporus volvatus (Peck) Hubb grows wild in China, and its fruiting 
      bodies have been used traditionally to treat asthma and bronchitis. OBJECTIVES: 
      This study evaluates the anti-inflammatory effect of Cryptoporus polysaccharides 
      (CP) extracted from fruiting bodies of C. volvatus on lipopolysaccharide 
      (LPS)-induced pro-inflammatory factors and the signaling pathways involved in 
      human alveolar epithelial cells. MATERIALS AND METHODS: To evaluate the effects 
      of CP on LPS-induced pro-inflammatory factors, A549 cells were pre-incubated with 
      CP 1, 10, and 100 mug/ml for 1 h and then stimulated with LPS 10 mug/ml for 24 h. 
      The expression of pro-inflammatory factors monocyte chemoattractant protein-1 
      (MCP-1), tumor necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta), Toll-like 
      receptor 2 (TLR2), and phosphorylation of ERK1/2, p38, and NF-kappaB p65 were 
      measured by q-PCR, ELISA, and western blotting. RESULTS: CP decreased LPS-induced 
      mRNA expression of MCP-1, TNF-alpha, and IL-1beta (IC50 = 83.3, 85.2, and 91.6 mug/ml, 
      respectively) and their correspondent protein expression (IC50 = 88.6, 76.4, and 
      81.6 mug/ml, respectively). Investigation of potential mechanisms indicated that 
      CP 100 mug/ml reduced LPS-induced expression of TLR2 mRNA (66.9%, p < 0.01) and 
      protein (63.2%, p < 0.01) that was a result of the decreased pro-inflammatory 
      factors. LPS induction increased the expression of TLR2 and the phosphorylation 
      of p38 and ERK1/2, NF-kB p65 concomitantly. CP 100 mug/ml inhibited the 
      LPS-induced phosphorylation of the signaling proteins (p < 0.05). CONCLUSIONS: 
      This suggests that CP pretreatment down-regulates LPS-mediated inflammation in 
      lung epithelial cells. This study further confirmed that CP is a potential 
      anti-inflammatory drug for the treatment of airway inflammatory diseases.
FAU - Zhu, Jian-Ping
AU  - Zhu JP
AD  - a Affiliated Sir Run Run Shaw Hospital, Zhejiang University School of Medicine , 
      Hangzhou , China .
FAU - Wu, Kai
AU  - Wu K
AD  - b Zhejiang Respiratory Drugs Research Laboratory of State Food and Drug 
      Administration of China, Zhejiang University School of Medicine , Hangzhou , 
      China , and.
AD  - c Animal Laboratoy Center of Zhejiang , Hangzhou , China.
FAU - Li, Jin-You
AU  - Li JY
AD  - b Zhejiang Respiratory Drugs Research Laboratory of State Food and Drug 
      Administration of China, Zhejiang University School of Medicine , Hangzhou , 
      China , and.
FAU - Guan, Yan
AU  - Guan Y
AD  - a Affiliated Sir Run Run Shaw Hospital, Zhejiang University School of Medicine , 
      Hangzhou , China .
AD  - b Zhejiang Respiratory Drugs Research Laboratory of State Food and Drug 
      Administration of China, Zhejiang University School of Medicine , Hangzhou , 
      China , and.
FAU - Sun, Yan-Hong
AU  - Sun YH
AD  - b Zhejiang Respiratory Drugs Research Laboratory of State Food and Drug 
      Administration of China, Zhejiang University School of Medicine , Hangzhou , 
      China , and.
FAU - Ma, Wen-Jiang
AU  - Ma WJ
AD  - b Zhejiang Respiratory Drugs Research Laboratory of State Food and Drug 
      Administration of China, Zhejiang University School of Medicine , Hangzhou , 
      China , and.
FAU - Xie, Qiang-Min
AU  - Xie QM
AD  - b Zhejiang Respiratory Drugs Research Laboratory of State Food and Drug 
      Administration of China, Zhejiang University School of Medicine , Hangzhou , 
      China , and.
AD  - c Animal Laboratoy Center of Zhejiang , Hangzhou , China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150512
PL  - England
TA  - Pharm Biol
JT  - Pharmaceutical biology
JID - 9812552
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Cytokines)
RN  - 0 (Fungal Polysaccharides)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (TLR2 protein, human)
RN  - 0 (Toll-Like Receptor 2)
SB  - IM
MH  - Anti-Inflammatory Agents/isolation & purification/*pharmacology
MH  - Blotting, Western
MH  - Cell Culture Techniques
MH  - Cell Line
MH  - Cell Survival/drug effects
MH  - Coriolaceae/*chemistry
MH  - Cytokines/*genetics/immunology
MH  - Dose-Response Relationship, Drug
MH  - Epithelial Cells/*drug effects/immunology
MH  - Fungal Polysaccharides/isolation & purification/*pharmacology
MH  - Humans
MH  - Lipopolysaccharides/pharmacology
MH  - Pulmonary Alveoli/cytology/*drug effects/immunology
MH  - Signal Transduction
MH  - Time Factors
MH  - Toll-Like Receptor 2/*metabolism
OTO - NOTNLM
OT  - Anti-inflammatory drug
OT  - asthma
OT  - bronchitis
OT  - interleukin-1beta
OT  - monocyte chemoattractant protein-1
OT  - tumor necrosis factor-alpha
EDAT- 2015/05/13 06:00
MHDA- 2016/09/24 06:00
CRDT- 2015/05/13 06:00
PHST- 2015/05/13 06:00 [entrez]
PHST- 2015/05/13 06:00 [pubmed]
PHST- 2016/09/24 06:00 [medline]
AID - 10.3109/13880209.2015.1042981 [doi]
PST - ppublish
SO  - Pharm Biol. 2016;54(2):347-53. doi: 10.3109/13880209.2015.1042981. Epub 2015 May 
      12.