PMID- 25963324
OWN - NLM
STAT- MEDLINE
DCOM- 20150930
LR  - 20181113
IS  - 1573-2568 (Electronic)
IS  - 0163-2116 (Linking)
VI  - 60
IP  - 8
DP  - 2015 Aug
TI  - Brain-Derived Neurotrophic Factor Contributes to Colonic Hypermotility in a 
      Chronic Stress Rat Model.
PG  - 2316-26
LID - 10.1007/s10620-015-3695-8 [doi]
AB  - BACKGROUND: Brain-derived neurotrophic factor (BDNF) has prokinetic effects on 
      gut motility and is increased in the colonic mucosa of irritable bowel syndrome. 
      AIMS: We aimed to investigate the possible involvement of BDNF in stress-induced 
      colonic hypermotility. METHODS: Male Wistar rats were exposed to daily 1-h water 
      avoidance stress (WAS) or sham WAS for 10 consecutive days. The presence of BDNF 
      and substance P (SP) in the colonic mucosa was determined using enzyme 
      immunoassay kits. Immunohistochemistry and western blotting were performed to 
      assess the expression of BDNF and its receptor, TrkB. The contractions of muscle 
      strips were studied in an organ bath system. RESULTS: Repeated WAS increased the 
      fecal pellet expulsion and spontaneous contractile activities of the colonic 
      muscle strips. Both BDNF and SP in the colonic mucosa were elevated following 
      WAS. Immunohistochemistry revealed the presence of BDNF and TrkB in the mucosa 
      and myenteric plexus. BDNF and TrkB were both up-regulated in colon devoid of 
      mucosa and submucosa from the stressed rats compared with the control. BDNF 
      pretreatment caused an enhancement of the SP-induced contraction of the circular 
      muscle (CM) strips. TrkB antibody significantly inhibited the contraction of the 
      colonic muscle strips and attenuated the excitatory effects of SP on contractions 
      of the CM strips. Repeated WAS increased the contractile activities of the CM 
      strips induced by SP after BDNF pretreatment, and this effect was reversed by 
      TrkB antibody. CONCLUSIONS: The colonic hypermotility induced by repeated WAS may 
      be associated with the increased expression of endogenous BDNF and TrkB. BDNF may 
      have potential clinical therapeutic use in modulating gut motility.
FAU - Quan, Xiaojing
AU  - Quan X
AD  - Department of Gastroenterology, Renmin Hospital of Wuhan University, Number 238, 
      Jiefang Road, Wuhan, 430060, Hubei Province, China, quanxiaojing77@126.com.
FAU - Luo, Hesheng
AU  - Luo H
FAU - Fan, Han
AU  - Fan H
FAU - Tang, Qincai
AU  - Tang Q
FAU - Chen, Wei
AU  - Chen W
FAU - Cui, Ning
AU  - Cui N
FAU - Yu, Guang
AU  - Yu G
FAU - Xia, Hong
AU  - Xia H
LA  - eng
PT  - Journal Article
DEP - 20150512
PL  - United States
TA  - Dig Dis Sci
JT  - Digestive diseases and sciences
JID - 7902782
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Membrane Glycoproteins)
RN  - 33507-63-0 (Substance P)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Brain-Derived Neurotrophic Factor/metabolism/*physiology
MH  - Colon/*physiology
MH  - Gastrointestinal Motility/*physiology
MH  - Immunohistochemistry
MH  - In Vitro Techniques
MH  - Intestinal Mucosa/metabolism
MH  - Male
MH  - Membrane Glycoproteins/physiology
MH  - Models, Animal
MH  - Muscle Contraction/physiology
MH  - Muscle, Smooth/physiology
MH  - Rats, Wistar
MH  - Stress, Psychological
MH  - Substance P/metabolism
MH  - Up-Regulation/physiology
EDAT- 2015/05/13 06:00
MHDA- 2015/10/01 06:00
CRDT- 2015/05/13 06:00
PHST- 2014/12/28 00:00 [received]
PHST- 2015/04/29 00:00 [accepted]
PHST- 2015/05/13 06:00 [entrez]
PHST- 2015/05/13 06:00 [pubmed]
PHST- 2015/10/01 06:00 [medline]
AID - 10.1007/s10620-015-3695-8 [doi]
PST - ppublish
SO  - Dig Dis Sci. 2015 Aug;60(8):2316-26. doi: 10.1007/s10620-015-3695-8. Epub 2015 
      May 12.