PMID- 2596614
OWN - NLM
STAT- MEDLINE
DCOM- 19900112
LR  - 20181130
IS  - 0002-9513 (Print)
IS  - 0002-9513 (Linking)
VI  - 257
IP  - 5 Pt 1
DP  - 1989 Nov
TI  - Accumulation of weak base in gastric mucosa provides evidence for an acidic 
      storage compartment.
PG  - G836-44
AB  - Uptake and release of acridine orange (AO), a fluorescent weak base that 
      accumulates in acidic spaces, were studied in perfused frog gastric mucosa. 
      Tissue was mounted between two flow-through chambers and loaded with AO on the 
      mucosal side. AO washout and acid secretion rate were monitored simultaneously by 
      a flow-through fluorescence detector and a pH-stat, respectively. Data were 
      displayed on a computer screen, stored, and analyzed. AO, in concentrations as 
      high as 0.02 mM, does not affect the acid secretion rate. Nonlinear least-squares 
      analysis of AO washout curves resolved two exponential components: a faster 
      component associated mainly with AO washout from the chamber and a slower 
      component reflecting primarily AO washout from the tissue. The slower exponential 
      declines more slowly at higher concentrations and/or longer duration of AO 
      loading, whereas the faster exponential is unaffected. AO washout is unaffected 
      by the level of the steady-state acid secretion rate. Nitrite inhibits the acid 
      secretion rate but does not affect the AO washout. When nitrite is removed, acid 
      secretion rate and fluorescence (AO concentration in the mucosal medium) increase 
      simultaneously and transiently. The net amount of AO released from the tissue is 
      proportional to the net amount of acid released. Stimulation by secretagogue in 
      basally secreting tissue causes synchronous transient increases in acid secretion 
      rate and fluorescence. We conclude that accumulation of AO provides evidence for 
      the existence of an intracellular storage pool of free protons within the 
      transporting epithelium.
FAU - Ekblad, E B
AU  - Ekblad EB
AD  - Cardiovascular Research Institute and Liver Center, University of California, San 
      Francisco 94143.
FAU - Warnock, D G
AU  - Warnock DG
FAU - Licko, V
AU  - Licko V
LA  - eng
GR  - 1-RO1-DK-39578/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Physiol
JT  - The American journal of physiology
JID - 0370511
RN  - 0 (Nitrites)
RN  - 820484N8I3 (Histamine)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange/*metabolism/pharmacology
MH  - Animals
MH  - Fluorescence
MH  - Gastric Acid/*metabolism
MH  - Gastric Mucosa/*metabolism
MH  - Histamine/pharmacology
MH  - Nitrites/pharmacology
MH  - Osmolar Concentration
MH  - Rana catesbeiana
MH  - Time Factors
EDAT- 1989/11/01 00:00
MHDA- 1989/11/01 00:01
CRDT- 1989/11/01 00:00
PHST- 1989/11/01 00:00 [pubmed]
PHST- 1989/11/01 00:01 [medline]
PHST- 1989/11/01 00:00 [entrez]
AID - 10.1152/ajpgi.1989.257.5.G836 [doi]
PST - ppublish
SO  - Am J Physiol. 1989 Nov;257(5 Pt 1):G836-44. doi: 10.1152/ajpgi.1989.257.5.G836.