PMID- 25966418
OWN - NLM
STAT- MEDLINE
DCOM- 20160217
LR  - 20150513
IS  - 1873-3573 (Electronic)
IS  - 0039-9140 (Linking)
VI  - 141
DP  - 2015 Aug 15
TI  - A highly selective and sensitive electrochemical CS-MWCNTs/Au-NPs composite DNA 
      biosensor for Staphylococcus aureus gene sequence detection.
PG  - 300-6
LID - S0039-9140(15)00205-2 [pii]
LID - 10.1016/j.talanta.2015.03.052 [doi]
AB  - This paper presents a new electrochemical DNA biosensor constructed using a 
      substrate electrode composed of a novel nanocomposite material prepared using 
      gold nanoparticles (Au-NPs) and multiwalled carbon nanotubes (MWCNTs) and further 
      modified with an Au electrode (AuE), which was used as the substrate electrode. A 
      single-stranded DNA (ssDNA) probe was immobilized on the Au-NPs/CS-MWCNTs/AuE 
      electrode by means of facile gold-thiol affinity, which resulted in hybridization 
      with the target ssDNA sequence. Hybridization reactions were assessed by using 
      the reduction peak current of methylene blue (MB) as an electrochemical 
      indicator. The advantages of the nanomaterials were found to include high surface 
      area, favorable electronic properties, and strong electrocatalytic activity. The 
      amount of ssDNA adsorbed on the electrode surface was increased and the 
      electrochemical response of MB accelerated. The differential pulse voltammetric 
      responses of MB were in line with the specific target ssDNA sequence within the 
      concentration range 1.0x10(-15)-1.0x10(-8)M with the detection limit 3.3x10(-16)M 
      (3sigma). In the colony forming unit (CFU) we were able to detect 10CFU mL(-1)of 
      Staphylococcus aureus in the tap water, achieving good discrimination ability 
      between one- and three-base mismatched ssDNA sequences. The polymerase chain 
      reaction (PCR) amplification products of S. aureus nuc gene sequence were also 
      detected with satisfactory results.
CI  - Copyright (c) 2015 Elsevier B.V. All rights reserved.
FAU - Sun, Yange
AU  - Sun Y
AD  - State Key Laboratory of Food Science and Technology, School of Food Science and 
      Technology, Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan 
      University, Wuxi, Jiangsu 214122, China.
FAU - He, Xingxing
AU  - He X
AD  - State Key Laboratory of Food Science and Technology, School of Food Science and 
      Technology, Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan 
      University, Wuxi, Jiangsu 214122, China.
FAU - Ji, Jian
AU  - Ji J
AD  - State Key Laboratory of Food Science and Technology, School of Food Science and 
      Technology, Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan 
      University, Wuxi, Jiangsu 214122, China.
FAU - Jia, Min
AU  - Jia M
AD  - State Key Laboratory of Food Science and Technology, School of Food Science and 
      Technology, Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan 
      University, Wuxi, Jiangsu 214122, China.
FAU - Wang, Zhouping
AU  - Wang Z
AD  - State Key Laboratory of Food Science and Technology, School of Food Science and 
      Technology, Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan 
      University, Wuxi, Jiangsu 214122, China.
FAU - Sun, Xiulan
AU  - Sun X
AD  - State Key Laboratory of Food Science and Technology, School of Food Science and 
      Technology, Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan 
      University, Wuxi, Jiangsu 214122, China. Electronic address: 
      sxlzzz@jiangnan.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150401
PL  - Netherlands
TA  - Talanta
JT  - Talanta
JID - 2984816R
RN  - 0 (Bacterial Proteins)
RN  - 0 (Immobilized Nucleic Acids)
RN  - 0 (Nanotubes, Carbon)
RN  - 0 (nuc protein, staphylococcus)
RN  - 7440-57-5 (Gold)
RN  - EC 3.1.31.1 (Micrococcal Nuclease)
RN  - T42P99266K (Methylene Blue)
SB  - IM
MH  - Animals
MH  - Bacterial Proteins/genetics
MH  - Biosensing Techniques/instrumentation/*methods
MH  - Calibration
MH  - Electrochemical Techniques/instrumentation/*methods
MH  - Electrodes
MH  - Equipment Design
MH  - Gold/chemistry
MH  - Immobilized Nucleic Acids
MH  - *Methylene Blue/chemistry
MH  - Micrococcal Nuclease/genetics
MH  - Milk/microbiology
MH  - Nanoparticles/chemistry
MH  - Nanotubes, Carbon
MH  - Polymerase Chain Reaction
MH  - Sensitivity and Specificity
MH  - Staphylococcus aureus/*genetics
MH  - Water Microbiology
OTO - NOTNLM
OT  - DNA biosensor
OT  - Gold nanoparticles
OT  - MWCNTs
OT  - Methylene blue (MB)
OT  - Staphylococcus aureus
EDAT- 2015/05/13 06:00
MHDA- 2016/02/18 06:00
CRDT- 2015/05/13 06:00
PHST- 2015/01/13 00:00 [received]
PHST- 2015/03/19 00:00 [revised]
PHST- 2015/03/24 00:00 [accepted]
PHST- 2015/05/13 06:00 [entrez]
PHST- 2015/05/13 06:00 [pubmed]
PHST- 2016/02/18 06:00 [medline]
AID - S0039-9140(15)00205-2 [pii]
AID - 10.1016/j.talanta.2015.03.052 [doi]
PST - ppublish
SO  - Talanta. 2015 Aug 15;141:300-6. doi: 10.1016/j.talanta.2015.03.052. Epub 2015 Apr 
      1.