PMID- 25966675
OWN - NLM
STAT- MEDLINE
DCOM- 20160704
LR  - 20150929
IS  - 1552-4965 (Electronic)
IS  - 1549-3296 (Linking)
VI  - 103
IP  - 11
DP  - 2015 Nov
TI  - Biological responses of preosteoblasts to particulate and ion forms of Co-Cr 
      alloy.
PG  - 3564-71
LID - 10.1002/jbm.a.35501 [doi]
AB  - This study compared the particulate and ion forms of a cobalt-chrome (Co-Cr) 
      alloy on the differentiation/activation of preosteoblasts. Mouse preosteoblasts 
      (MC3T3-E1) were cultured in an osteoblast-induction medium in the presence of 
      particulate and ion forms of a Co-Cr alloy, followed by cell proliferation and 
      cytotoxicity evaluations. The maturation and function of osteoblasts were 
      assessed by alkaline phosphatase (ALP) assay and related gene expressions. Both 
      particulate and ion forms of the metals significantly reduced the proliferation 
      of MC3T3-E1 cells in a dose-dependent manner. Similarly, cells challenged with 
      high concentrations of particles and ions exhibited a marked cytotoxic effect and 
      diminished expression of ALP. Real-time (RT) polymerase chain reaction (PCR) data 
      have suggested that cells with Co-Cr particles dramatically promoted 
      over-expression of monocyte chemo-attractant protein-1 (MCP-1), tumor necrosis 
      factor-alpha (TNF-alpha), and interleukin-6 (IL-6), whereas Co(2+) ions treatment 
      predominately up-regulated expressions of receptor activator of nuclear factor 
      kappa-B ligand (RANKL), nuclear factor of activated T-cells cytoplasmic 1 
      (NFATc1), and down-regulated expression of osteoprotegerin (OPG) and Osterix 
      (Osx). Overall, this study provides evidence that both Co-Cr alloy particles and 
      metal ions interfered with the MC3T3-E1 cells for their growth, maturation, and 
      functions. Further, Co-Cr particles exhibited stronger effects on inflammatory 
      mediators, while metal ions showed more influence on inhibition of osteoblast 
      differentiation and promotion of osteoclastogenesis.
CI  - (c) 2015 Wiley Periodicals, Inc.
FAU - Yang, Shuye
AU  - Yang S
AD  - Department of Orthopaedic Surgery, Jinan Central Hospital, Shandong University, 
      Jinan, 250013, China.
AD  - Department of Biological Sciences, Wichita State University, Wichita, Kansas, 
      67214.
AD  - Department of Orthopaedics, Affiliated Hospital to Binzhou Medical College, 
      Binzhou, China.
FAU - Zhang, Kai
AU  - Zhang K
AD  - Department of Orthopaedics, Affiliated Hospital to Binzhou Medical College, 
      Binzhou, China.
FAU - Li, Fangfang
AU  - Li F
AD  - Department of Gynaecology and Obstetrics, Affiliated Hospital to Binzhou Medical 
      College, Binzhou, China.
FAU - Jiang, Jianhao
AU  - Jiang J
AD  - Department of Orthopaedic Surgery, Jinan Central Hospital, Shandong University, 
      Jinan, 250013, China.
FAU - Jia, Tanghong
AU  - Jia T
AD  - Department of Orthopaedic Surgery, Jinan Central Hospital, Shandong University, 
      Jinan, 250013, China.
FAU - Yang, Shang-You
AU  - Yang SY
AD  - Department of Orthopaedic Surgery, Jinan Central Hospital, Shandong University, 
      Jinan, 250013, China.
AD  - Department of Biological Sciences, Wichita State University, Wichita, Kansas, 
      67214.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150527
PL  - United States
TA  - J Biomed Mater Res A
JT  - Journal of biomedical materials research. Part A
JID - 101234237
RN  - 0 (Chromium Alloys)
RN  - 0 (Ions)
RN  - 0 (Particulate Matter)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
SB  - IM
MH  - Alkaline Phosphatase/metabolism
MH  - Animals
MH  - Cell Death/drug effects
MH  - Cell Line
MH  - Cell Proliferation/drug effects
MH  - Cell Shape/drug effects
MH  - Chromium Alloys/*pharmacology
MH  - Gene Expression Profiling
MH  - Ions
MH  - L-Lactate Dehydrogenase/metabolism
MH  - Mice
MH  - Osteoblasts/*cytology/drug effects/enzymology
MH  - Particulate Matter/*pharmacology
MH  - Real-Time Polymerase Chain Reaction
OTO - NOTNLM
OT  - aseptic loosening
OT  - differentiation
OT  - metal ions
OT  - preosteoblasts
OT  - wear debris
EDAT- 2015/05/15 06:00
MHDA- 2016/07/05 06:00
CRDT- 2015/05/14 06:00
PHST- 2015/03/23 00:00 [received]
PHST- 2015/05/02 00:00 [revised]
PHST- 2015/05/07 00:00 [accepted]
PHST- 2015/05/14 06:00 [entrez]
PHST- 2015/05/15 06:00 [pubmed]
PHST- 2016/07/05 06:00 [medline]
AID - 10.1002/jbm.a.35501 [doi]
PST - ppublish
SO  - J Biomed Mater Res A. 2015 Nov;103(11):3564-71. doi: 10.1002/jbm.a.35501. Epub 
      2015 May 27.