PMID- 25968357
OWN - NLM
STAT- MEDLINE
DCOM- 20160509
LR  - 20170815
IS  - 1878-7568 (Electronic)
IS  - 1742-7061 (Linking)
VI  - 23
DP  - 2015 Sep
TI  - Freeze gelated porous membranes for periodontal tissue regeneration.
PG  - 317-328
LID - S1742-7061(15)00218-4 [pii]
LID - 10.1016/j.actbio.2015.05.001 [doi]
AB  - Guided tissue regeneration (GTR) membranes have been used for the management of 
      destructive forms of periodontal disease as a means of aiding regeneration of 
      lost supporting tissues, including the alveolar bone, cementum, gingiva and 
      periodontal ligaments (PDL). Currently available GTR membranes are either 
      non-biodegradable, requiring a second surgery for removal, or biodegradable. The 
      mechanical and biofunctional limitations of currently available membranes result 
      in a limited and unpredictable treatment outcome in terms of periodontal tissue 
      regeneration. In this study, porous membranes of chitosan (CH) were fabricated 
      with or without hydroxyapatite (HA) using the simple technique of freeze gelation 
      (FG) via two different solvents systems, acetic acid (ACa) or ascorbic acid 
      (ASa). The aim was to prepare porous membranes to be used for GTR to improve 
      periodontal regeneration. FG membranes were characterized for ultra-structural 
      morphology, physiochemical properties, water uptake, degradation, mechanical 
      properties, and biocompatibility with mature and progenitor osteogenic cells. 
      Fourier transform infrared (FTIR) spectroscopy confirmed the presence of 
      hydroxyapatite and its interaction with chitosan. muCT analysis showed membranes 
      had 85-77% porosity. Mechanical properties and degradation rate were affected by 
      solvent type and the presence of hydroxyapatite. Culture of human osteosarcoma 
      cells (MG63) and human embryonic stem cell-derived mesenchymal progenitors 
      (hES-MPs) showed that all membranes supported cell proliferation and long term 
      matrix deposition was supported by HA incorporated membranes. These CH and HA 
      composite membranes show their potential use for GTR applications in periodontal 
      lesions and in addition FG membranes could be further tuned to achieve 
      characteristics desirable of a GTR membrane for periodontal regeneration.
CI  - Copyright (c) 2015 Acta Materialia Inc. Published by Elsevier Ltd. All rights 
      reserved.
FAU - Qasim, Saad B
AU  - Qasim SB
AD  - Materials Science and Engineering Department, Kroto Research Institute, 
      University of Sheffield, Sheffield S3 7HQ, United Kingdom.
FAU - Delaine-Smith, Robin M
AU  - Delaine-Smith RM
AD  - Institute of Bioengineering, School of Engineering and Materials Science, Queen 
      Mary University of London, Mile End Road, E1 4NS London, United Kingdom.
FAU - Fey, Tobias
AU  - Fey T
AD  - Department of Materials Science (Glass and Ceramics), University of 
      Erlangen-Nuernberg, Martensstr. 5, 91058 Erlangen, Germany.
FAU - Rawlinson, Andrew
AU  - Rawlinson A
AD  - Academic Unit of Restorative Dentistry, School of Clinical Dentistry, University 
      of Sheffield, Sheffield S10 2SZ, United Kingdom.
FAU - Rehman, Ihtesham Ur
AU  - Rehman IU
AD  - Materials Science and Engineering Department, Kroto Research Institute, 
      University of Sheffield, Sheffield S3 7HQ, United Kingdom. Electronic address: 
      i.u.rehman@sheffield.ac.uk.
LA  - eng
PT  - Journal Article
DEP - 20150509
PL  - England
TA  - Acta Biomater
JT  - Acta biomaterialia
JID - 101233144
RN  - 0 (Gels)
RN  - 0 (Membranes, Artificial)
RN  - 9012-76-4 (Chitosan)
RN  - 91D9GV0Z28 (Durapatite)
SB  - IM
MH  - Cell Proliferation/physiology
MH  - Cells, Cultured
MH  - Chitosan/chemistry
MH  - Durapatite/chemistry
MH  - Elastic Modulus
MH  - Equipment Design
MH  - Equipment Failure Analysis
MH  - Freezing
MH  - Gels/*chemistry
MH  - Guided Tissue Regeneration, Periodontal/*instrumentation/methods
MH  - Humans
MH  - Materials Testing
MH  - *Membranes, Artificial
MH  - Osteoblasts/*cytology/physiology
MH  - Osteogenesis/physiology
MH  - Porosity
MH  - Stem Cells/*cytology/physiology
MH  - Stress, Mechanical
MH  - Tensile Strength
MH  - *Tissue Scaffolds
OTO - NOTNLM
OT  - Ascorbic acid
OT  - Bioactivity
OT  - Guided tissue regeneration
OT  - Osteoblasts
OT  - Resorbable
EDAT- 2015/05/15 06:00
MHDA- 2016/05/10 06:00
CRDT- 2015/05/14 06:00
PHST- 2015/01/11 00:00 [received]
PHST- 2015/04/02 00:00 [revised]
PHST- 2015/05/04 00:00 [accepted]
PHST- 2015/05/14 06:00 [entrez]
PHST- 2015/05/15 06:00 [pubmed]
PHST- 2016/05/10 06:00 [medline]
AID - S1742-7061(15)00218-4 [pii]
AID - 10.1016/j.actbio.2015.05.001 [doi]
PST - ppublish
SO  - Acta Biomater. 2015 Sep;23:317-328. doi: 10.1016/j.actbio.2015.05.001. Epub 2015 
      May 9.