PMID- 25968442
OWN - NLM
STAT- MEDLINE
DCOM- 20160225
LR  - 20220408
IS  - 1421-9778 (Electronic)
IS  - 1015-8987 (Linking)
VI  - 36
IP  - 2
DP  - 2015
TI  - Effect of EphA7 Silencing on Proliferation, Invasion and Apoptosis in Human 
      Laryngeal Cancer Cell Lines Hep-2 and AMC-HN-8.
PG  - 435-45
LID - 10.1159/000430110 [doi]
AB  - AIMS: This study aimed to investigate the expression of EphA7 in human laryngeal 
      squamous cell carcinoma (LSCC) tissues and disclose the potential roles and 
      molecular mechanisms of EphA7 in LSCC. METHODS: In the present study, we examined 
      EphA7 expression and its function and mechanism in LSCC. EphA7 expression levels 
      were investigated by quantitative real-time PCR (qRT-PCR), western blotting, and 
      immunohistochemistry in a panel of 35 LSCC patient cases. To investigate the 
      potential mechanism of EphA7 in human laryngeal cancer, we employed EphA7 siRNA 
      to knockdown EphA7 expression in LSCC cell line Hep-2 and AMC-HN-8. Subsequently, 
      MTT, TUNEL, qRT-PCR, and western blotting were performed to disclose the roles of 
      EphA7 on proliferation, invasion and migration, and apoptosis in LSCC cell line 
      Hep-2 and AMC-HN-8. RESULTS: Depletion of EphA7 remarkably inhibited the 
      proliferation and invasion of Hep-2 and AMC-HN-8 cells in comparison to control 
      and EphA7 siRNA negative control (NC)-transfected cells. TUNEL staining assay 
      demonstrated that, compared with the control group, the rate of apoptosis in the 
      EphA7 siRNA group was significantly increased. In addition, knockdown of EphA7 in 
      Hep-2 or AMC-HN-8 cells markedly decreased the expression of EphA7 and PTEN, 
      which could contribute to apoptosis. However, the bpV(phen), a PTEN inhibitor, 
      could attenuate anti-proliferation and pro-apoptotic effects of EphA7 siRNA in 
      Hep-2 and AMC-HN-8 cells. CONCLUSION: Up-regulation of EphA7 was observed in 
      human LSCC samples and down-regulation of EphA7 effectively suppressed laryngeal 
      carcinoma cell growth and promoted its apoptosis. Thus, EphA7 has a critical role 
      in modulating cell growth and apoptosis, which serves as a potential therapeutic 
      target in human LSCC.
CI  - (c) 2015 S. Karger AG, Basel.
FAU - Xiang, Cheng
AU  - Xiang C
AD  - Department of Head and Neck Surgery, the Third Affiliated Hospital of Harbin 
      Medical University, Harbin, China.
FAU - Lv, Yuanjing
AU  - Lv Y
FAU - Wei, Yanjie
AU  - Wei Y
FAU - Wei, Jing
AU  - Wei J
FAU - Miao, Susheng
AU  - Miao S
FAU - Mao, Xionghui
AU  - Mao X
FAU - Gu, Xin
AU  - Gu X
FAU - Song, Kaibin
AU  - Song K
FAU - Jia, Shenshan
AU  - Jia S
LA  - eng
PT  - Journal Article
DEP - 20150511
PL  - Germany
TA  - Cell Physiol Biochem
JT  - Cellular physiology and biochemistry : international journal of experimental 
      cellular physiology, biochemistry, and pharmacology
JID - 9113221
RN  - 0 (RNA, Small Interfering)
RN  - EC 2.7.10.1 (Receptor, EphA7)
SB  - IM
EIN - Cell Physiol Biochem. 2015;36(5):2084
MH  - Apoptosis
MH  - Carcinoma, Squamous Cell/*genetics/pathology/therapy
MH  - Cell Cycle
MH  - Cell Line, Tumor
MH  - Cell Proliferation
MH  - *Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Laryngeal Neoplasms/*genetics/pathology/therapy
MH  - Larynx/metabolism/*pathology
MH  - Neoplasm Invasiveness/*genetics/pathology/prevention & control
MH  - RNA Interference
MH  - RNA, Small Interfering/genetics/therapeutic use
MH  - *RNAi Therapeutics
MH  - Receptor, EphA7/*genetics
MH  - Up-Regulation
EDAT- 2015/05/15 06:00
MHDA- 2016/02/26 06:00
CRDT- 2015/05/14 06:00
PHST- 2015/02/20 00:00 [accepted]
PHST- 2015/05/14 06:00 [entrez]
PHST- 2015/05/15 06:00 [pubmed]
PHST- 2016/02/26 06:00 [medline]
AID - 000430110 [pii]
AID - 10.1159/000430110 [doi]
PST - ppublish
SO  - Cell Physiol Biochem. 2015;36(2):435-45. doi: 10.1159/000430110. Epub 2015 May 
      11.