PMID- 25970091 OWN - NLM STAT- MEDLINE DCOM- 20160316 LR - 20191008 IS - 1559-2308 (Electronic) IS - 1559-2294 (Print) IS - 1559-2294 (Linking) VI - 10 IP - 7 DP - 2015 TI - Effects of particulate matter exposure on blood 5-hydroxymethylation: results from the Beijing truck driver air pollution study. PG - 633-42 LID - 10.1080/15592294.2015.1050174 [doi] AB - Previous studies have reported epigenetic changes induced by environmental exposures. However, previous investigations did not distinguish 5-methylcytosine (5mC) from a similar oxidative form with opposite functions, 5-hydroxymethylcytosine (5hmC). Here, we measured blood DNA global 5mC and 5hmC by ELISA and used adjusted mixed-effects regression models to evaluate the effects of ambient PM10 and personal PM2.5 and its elemental components-black carbon (BC), aluminum (Al), calcium (Ca), potassium (K), iron (Fe), sulfur (S), silicon (Si), titanium (Ti), and zinc (Zn)-on blood global 5mC and 5hmC levels. The study was conducted in 60 truck drivers and 60 office workers in Beijing, China from The Beijing Truck Driver Air Pollution Study at 2 exams separated by one to 2 weeks. Blood 5hmC level (0.08%) was approximately 83-fold lower than 5mC (6.61%). An inter-quartile range (IQR) increase in same-day PM10 was associated with increases in 5hmC of 26.1% in office workers (P = 0.004), 20.2% in truck drivers (P = 0.014), and 21.9% in all participants combined (P < 0.001). PM10 effects on 5hmC were increasingly stronger when averaged over 4, 7, and 14 d preceding assessment (up to 132.6% for the 14-d average in all participants, P < 0.001). PM10 effects were also significant after controlling for multiple testing (family-wise error rate; FWER < 0.05). 5hmC was not correlated with personal measures of PM2.5 and elemental components (FWER > 0.05). 5mC showed no correlations with PM10, PM2.5, and elemental components measures (FWER > 0.05). Our study suggests that exposure to ambient PM10 affects 5hmC over time, but not 5mC. This finding demonstrates the need to differentiate 5hmC and 5mC in environmental studies of DNA methylation. FAU - Sanchez-Guerra, Marco AU - Sanchez-Guerra M AD - a Department of Environmental Health; Harvard T.H. Chan School of Public Health ; Boston , MA , USA. FAU - Zheng, Yinan AU - Zheng Y FAU - Osorio-Yanez, Citlalli AU - Osorio-Yanez C FAU - Zhong, Jia AU - Zhong J FAU - Chervona, Yana AU - Chervona Y FAU - Wang, Sheng AU - Wang S FAU - Chang, Dou AU - Chang D FAU - McCracken, John P AU - McCracken JP FAU - Diaz, Anaite AU - Diaz A FAU - Bertazzi, Pier Alberto AU - Bertazzi PA FAU - Koutrakis, Petros AU - Koutrakis P FAU - Kang, Choong-Min AU - Kang CM FAU - Zhang, Xiao AU - Zhang X FAU - Zhang, Wei AU - Zhang W FAU - Byun, Hyang-Min AU - Byun HM FAU - Schwartz, Joel AU - Schwartz J FAU - Hou, Lifang AU - Hou L FAU - Baccarelli, Andrea A AU - Baccarelli AA LA - eng GR - R21 ES020984/ES/NIEHS NIH HHS/United States GR - ES00002/ES/NIEHS NIH HHS/United States GR - P30 ES000002/ES/NIEHS NIH HHS/United States GR - R21ES020984/ES/NIEHS NIH HHS/United States GR - R21ES020010/ES/NIEHS NIH HHS/United States GR - R21 ES020010/ES/NIEHS NIH HHS/United States GR - R01ES021733/ES/NIEHS NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, Non-U.S. Gov't PT - Research Support, U.S. Gov't, Non-P.H.S. PL - United States TA - Epigenetics JT - Epigenetics JID - 101265293 RN - 0 (Particulate Matter) RN - 1123-95-1 (5-hydroxymethylcytosine) RN - 6R795CQT4H (5-Methylcytosine) RN - 8J337D1HZY (Cytosine) SB - IM MH - 5-Methylcytosine/blood MH - Adolescent MH - Adult MH - Air Pollution/*adverse effects MH - Beijing MH - Cytosine/*analogs & derivatives/blood MH - Environmental Exposure/*adverse effects MH - Female MH - Humans MH - Male MH - Middle Aged MH - Particulate Matter/chemistry/metabolism/*toxicity MH - Young Adult PMC - PMC4623004 OTO - NOTNLM OT - 10 mum OT - 2.5 mum OT - 5-hydroxymethylcytosine OT - 5-methylcytosine OT - 5hmC, 5-hydroxymethylcytosine OT - 5mC, 5-methylcytosine OT - Al, aluminum OT - BC, black carbon OT - BMI, body mass index OT - CI, confidence interval OT - Ca, calcium OT - DNA methylation OT - ELISA, enzyme-linked immunosorbent assay OT - Epigenetics OT - FWER, family-wise error rate OT - Fe, iron OT - HPLC, high-performance liquid chromatography OT - K, potassium OT - PM, particulate matter OT - PM10, particulate matter